Spatial distribution and temporal evolution of DRONPA-fused SNAP25 clusters in adrenal chromaffin cells

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Spatial distribution and temporal evolution of DRONPA-fused SNAP25 clusters in adrenal chromaffin cells. / Antoku, Yasuko; Dedecker, Peter; da Silva Pinheiro, Paulo César; Vosch, Tom André Jos; Sørensen, Jakob Balslev.

In: Photochemical & Photobiological Sciences, Vol. 14, No. 5, 2015, p. 1005-1012.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Antoku, Y, Dedecker, P, da Silva Pinheiro, PC, Vosch, TAJ & Sørensen, JB 2015, 'Spatial distribution and temporal evolution of DRONPA-fused SNAP25 clusters in adrenal chromaffin cells', Photochemical & Photobiological Sciences, vol. 14, no. 5, pp. 1005-1012. https://doi.org/10.1039/c4pp00423j

APA

Antoku, Y., Dedecker, P., da Silva Pinheiro, P. C., Vosch, T. A. J., & Sørensen, J. B. (2015). Spatial distribution and temporal evolution of DRONPA-fused SNAP25 clusters in adrenal chromaffin cells. Photochemical & Photobiological Sciences, 14(5), 1005-1012. https://doi.org/10.1039/c4pp00423j

Vancouver

Antoku Y, Dedecker P, da Silva Pinheiro PC, Vosch TAJ, Sørensen JB. Spatial distribution and temporal evolution of DRONPA-fused SNAP25 clusters in adrenal chromaffin cells. Photochemical & Photobiological Sciences. 2015;14(5):1005-1012. https://doi.org/10.1039/c4pp00423j

Author

Antoku, Yasuko ; Dedecker, Peter ; da Silva Pinheiro, Paulo César ; Vosch, Tom André Jos ; Sørensen, Jakob Balslev. / Spatial distribution and temporal evolution of DRONPA-fused SNAP25 clusters in adrenal chromaffin cells. In: Photochemical & Photobiological Sciences. 2015 ; Vol. 14, No. 5. pp. 1005-1012.

Bibtex

@article{798832a4acad4a939d4828cb54617246,
title = "Spatial distribution and temporal evolution of DRONPA-fused SNAP25 clusters in adrenal chromaffin cells",
abstract = "Sub-diffraction imaging of plasma membrane localized proteins, such as the SNARE (Soluble NSF Attachment Protein Receptor) proteins involved in exocytosis, in fixed cells have resulted in images with high spatial resolution, at the expense of dynamical information. Here, we have imaged localized fluorescence bursts of DRONPA-fused SNAP-25 molecules in live chromaffin cells by Total Internal Reflection Fluorescence (TIRF) imaging. We find that this method allows tracking protein cluster dynamics over relatively long times (∼20 min.), partly due to the diffusion into the TIRF field of fresh molecules, making possible the simultaneous identification of cluster size, location and temporal evolution. The results indicate that the DRONPA-fused SNAP-25 clusters display rich dynamics, going from staying constant to disappearing and reappearing in specific cluster domains within minutes.",
author = "Yasuko Antoku and Peter Dedecker and {da Silva Pinheiro}, {Paulo C{\'e}sar} and Vosch, {Tom Andr{\'e} Jos} and S{\o}rensen, {Jakob Balslev}",
year = "2015",
doi = "10.1039/c4pp00423j",
language = "English",
volume = "14",
pages = "1005--1012",
journal = "Photochemical & Photobiological Sciences",
issn = "1474-905X",
publisher = "Royal Society of Chemistry",
number = "5",

}

RIS

TY - JOUR

T1 - Spatial distribution and temporal evolution of DRONPA-fused SNAP25 clusters in adrenal chromaffin cells

AU - Antoku, Yasuko

AU - Dedecker, Peter

AU - da Silva Pinheiro, Paulo César

AU - Vosch, Tom André Jos

AU - Sørensen, Jakob Balslev

PY - 2015

Y1 - 2015

N2 - Sub-diffraction imaging of plasma membrane localized proteins, such as the SNARE (Soluble NSF Attachment Protein Receptor) proteins involved in exocytosis, in fixed cells have resulted in images with high spatial resolution, at the expense of dynamical information. Here, we have imaged localized fluorescence bursts of DRONPA-fused SNAP-25 molecules in live chromaffin cells by Total Internal Reflection Fluorescence (TIRF) imaging. We find that this method allows tracking protein cluster dynamics over relatively long times (∼20 min.), partly due to the diffusion into the TIRF field of fresh molecules, making possible the simultaneous identification of cluster size, location and temporal evolution. The results indicate that the DRONPA-fused SNAP-25 clusters display rich dynamics, going from staying constant to disappearing and reappearing in specific cluster domains within minutes.

AB - Sub-diffraction imaging of plasma membrane localized proteins, such as the SNARE (Soluble NSF Attachment Protein Receptor) proteins involved in exocytosis, in fixed cells have resulted in images with high spatial resolution, at the expense of dynamical information. Here, we have imaged localized fluorescence bursts of DRONPA-fused SNAP-25 molecules in live chromaffin cells by Total Internal Reflection Fluorescence (TIRF) imaging. We find that this method allows tracking protein cluster dynamics over relatively long times (∼20 min.), partly due to the diffusion into the TIRF field of fresh molecules, making possible the simultaneous identification of cluster size, location and temporal evolution. The results indicate that the DRONPA-fused SNAP-25 clusters display rich dynamics, going from staying constant to disappearing and reappearing in specific cluster domains within minutes.

U2 - 10.1039/c4pp00423j

DO - 10.1039/c4pp00423j

M3 - Journal article

C2 - 25837695

VL - 14

SP - 1005

EP - 1012

JO - Photochemical & Photobiological Sciences

JF - Photochemical & Photobiological Sciences

SN - 1474-905X

IS - 5

ER -

ID: 137165277