Spatial distribution and temporal evolution of DRONPA-fused SNAP25 clusters in adrenal chromaffin cells
Research output: Contribution to journal › Journal article › peer-review
Documents
- Antoku_2015_Spatial_distribution_and_temporal
Final published version, 3.25 MB, PDF document
Sub-diffraction imaging of plasma membrane localized proteins, such as the SNARE (Soluble NSF Attachment Protein Receptor) proteins involved in exocytosis, in fixed cells have resulted in images with high spatial resolution, at the expense of dynamical information. Here, we have imaged localized fluorescence bursts of DRONPA-fused SNAP-25 molecules in live chromaffin cells by Total Internal Reflection Fluorescence (TIRF) imaging. We find that this method allows tracking protein cluster dynamics over relatively long times (∼20 min.), partly due to the diffusion into the TIRF field of fresh molecules, making possible the simultaneous identification of cluster size, location and temporal evolution. The results indicate that the DRONPA-fused SNAP-25 clusters display rich dynamics, going from staying constant to disappearing and reappearing in specific cluster domains within minutes.
Original language | English |
---|---|
Journal | Photochemical & Photobiological Sciences |
Volume | 14 |
Issue number | 5 |
Pages (from-to) | 1005-1012 |
Number of pages | 8 |
ISSN | 1474-905X |
DOIs | |
Publication status | Published - 2015 |
Number of downloads are based on statistics from Google Scholar and www.ku.dk
ID: 137165277