Associate Professor Jens Rekling
Department of Neuroscience
The Panum Institute, room 33-3-84
Phone: +45 3152 6540
The aim of the research is to understand how neurons in small assemblies interact to produce sensory and motor functions in the brain.
Associate Professor Jens Rekling
Department of Neuroscience
The Panum Institute, room 33-3-84
Phone: +45 3152 6540
The aim of the research is to understand how neurons in small assemblies interact to produce sensory and motor functions in the brain. A variety of electrophysiological and optical techniques are used to study living neurons in preparations of nervous tissue that maintain functional sensorimotor systems under in vitro conditions. We seek to answer fundamental questions such as: What neural microcircuit mechanisms in the brainstem are involved in generating breathing movements? How do sensory and motor systems produce precision movements? What developmental processes are involved in specifying functional microcircuits? Ultimately we wish to contribute to an understanding of how brain function emerges from network interactions between individual neurons.
En-block brainstem, acute slice, and slice culture preparations from newborn mice are used to study the cellular and system properties of respiratory neurons with the aim of understanding how breathing rhythm is generated.
Spontaneous respiratory activity on hypoglossal and cervical nerves in an in vitro brainstem-spinal cord preparation from postnatal mice.
Slice and whole-mount preparations from newborn mice are used to study coordinated calcium activity in neighboring groups of neurons with the aim of understanding early developmental specification of neural function.
Processing of sensory information and cerebellar function
Slice preparations from the inferior olive from newborn mice are used to study spontaneous coordinated activity in clusters of inferior olive neurons with the aim of understanding early olivocerebellar development and function. The internal processing in the cerebellar cortex is analyzed in the intact cerebellum. Of particular focus is the inner workings of mossy fibers, and the Golgi cell inhibitory control of the mossy fiber-granule cell synapse in the input layer of the cerebellar cortex.
Detection of clusters in the inferior olivary nucleus in a slice from postnatal mice. Movie showing concurrent image stacks of original Fluo-8, AM data, ∆F/F and 1 frame subtraction, and three stages of an automatic cluster detection algorithm as described in our 2013 paper in J. Physiol.
We are currently looking for students interested in doing their Bachelor, Master, or PhD project in our group. We will actively support the application of suitable students for scholarships from various sources. The projects might include both electrophysiological, optical and molecular biology techniques. If you are interested in becoming a member of our team, please contact:
Jens C. Rekling
phone: +45 3152 6540
Gold medal in the 1985/86 prize essay in medicine G (1986).
Medical Doctor (MD) from University of Copenhagen (1988).
Doctor of Medical Science (dr.med., DMSci) from University of Copenhagen (1999).
1982‑1985: Student. The bloodbank of Rigshospitalet.
1986‑1987: Student. Institute of Neurophysiology, University of Copenhagen funded by a scholarship by Upjohn.
1988-1993: Postdoctoral fellow. Institute of Neurophysiology, University of Copenhagen.
1993-1995: EU Fellow. Institute Alfred Fessard, C.N.R.S., France.
1995-1999: Assistant Research Scientist (Parker B. Francis fellow). UCLA - Department of Neurobiology.
1998-1999: Visiting Assistant Professor. UCLA - Department of Physiological Science.
1999-: Research Scientist. H. Lundbeck A/S.
2001-: Project leader. H. Lundbeck A/S.
2001-: Section head – Electrophysiology. H. Lundbeck A/S.
2003-2005: Principal Scientist – Electrophysiology. H. Lundbeck A/S.
2005-: Associate Professor (Lektor). Department of Medical Physiology.
2007-2011: Vice-Chair. Department of Neuroscience and Pharmacology.
34 Rekling, J.C., Mosfeldt Laursen, A. Evidence for a persistent sodium conductance in neurons from the nucleus prepositus hypoglossi. Brain Research. 1989; 500: 276-286.
33 Rekling, J.C., Jahnsen, H., Mosfeldt Laursen, A. The effect of two lipophilic GABA uptake blockers in CA1 of the rat hippocampal slice. Br. J. Pharmacol. 1990; 99: 103-106.
32 Rekling, J.C.. Excitatory effects of thyrotropin releasing hormone (TRH) in hypoglossal motoneurones. Brain Research. 1990; 510: 175-179.
31 Rekling, J.C. Interaction between thyrotropin-releasing hormone (TRH) and NMDA receptor mediated responses in hypoglossal motoneurones. Brain Research. 1992; 578 (1/2): 289-296.
30 Rekling, J.C. Effects of met-enkephalin on GABAergic spontaneous miniature IPSPs in organotypic slice cultures of the rat hippocampus. J. Neuroscience. 1993; 13: 1954-1964.
29 Rekling, J.C. and Theophilidis, G. Effects of a pyrethroid insecticide, deltamethrin, on respiratory modulated hypoglossal motoneurons in a brain stem slice from newborn mice. Neuroscience Letters. 1995; 198: 189-192.
28 Rekling, J.C. , Champagnat, J. and Denavit-Saubié, M. Electroresponsive properties and membrane potential trajectories of three types of inspiratory neurons in the newborn mouse brain stem in vitro. J. Neurophysiol. 1996; 75(2): 795-810.
27 Rekling, J.C., Champagnat, J. and Denavit-Saubié, M. Thyrotropin-releasing hormone (TRH) depolarizes a subset of inspiratory neurons in the newborn mouse brain stem in vitro. J. Neurophysiol. 1996; 75 (2): 811-819.
26 Møller, M., Micic, A., Rekling, J.C. Morphological characterization of rat pineal explant cultures. In: Neuroendocrinology: Retrospect and Perspectives (eds., H.-W. Korf and K.H. Usadel). chapter 27, pp.357-366. Springer-Verlag; Heidelberg, New York 1997.
25 Rekling, J.C. and Feldman, J. L. Calcium-dependent plateau potentials in rostral ambiguus neurons in the newborn mouse brain stem in vitro. J. Neurophysiol. 1997; 78: 2483-2492.
24 Rekling, J.C. and Feldman, J. L. Bidirectional electrical coupling between inspiratory motoneurons in the newborn mouse nucleus ambiguus. J. Neurophysiol. 1997; 78: 3508-3510.
23 Zheng, Y., Riche, D, Rekling, J.C., Foutz, A. S. and Denavit Saubié, M. Brainstem neurons projecting to the rostral ventral respiratory group (rVRG) in the medulla of the rat revealed by co application of NMDA and biocytin. Brain Research. 1998; 782 (1-2):113-125.
22 Rekling, J.C. and Feldman, J.L. Prebötzinger complex and pacemaker neurons: Hypothesized site and kernel for respiratory rhythm generation. Annual Review of Physiology. 1998; vol 60: 385-405.
21 Gray, P. A., Rekling, J.C., Bocchiaro, C. M., and Feldman, J. L. Modulation of respiratory frequency by peptidergic input to rhythmogenic neurons in the Prebötzinger complex. Science. 1999; 286 (5444): 1566-1568 (first and second author contributed equally to the work).
20 Rekling, J.C., Shao,X.M., and Feldman, J.L.. Electrical coupling and excitatory synaptic transmission between rhythmogenic respiratory neurons in the preBötzinger Complex. J. Neuroscience. 2000; 20(23): RC113, 1-5.
19 Rekling, J.C., Funk, G. D., Bayliss, D. A., Dong, X-W, and Feldman, J. L. Synaptic control of motoneuronal excitability. Physiological Reviews. 2000; 80(2): 767-852.
18 Rekling, J.C. Neuroprotective effects of anticonvulsants in rat hippocampal slice cultures exposed to oxygen/glucose deprivation. Neuroscience Letters. 2003; 335: 167-170.
17 Rekling, J.C. NK-3 receptor activation depolarizes and induces an afterdepolarization in pyramidal neurons in gerbil cingulate cortex. Brain Research Bulletin. 2004; 63 (2), 85-90.
16 Laursen, M and Rekling, J.C. The edinger-westphal nucleus of the juvenile rat contains transient- and repetitive-firing neurons. Neuroscience. 2006; 141: 191-200.
15 Falk, S, and Rekling, J.C. Neurons in the prebötzinger complex and VRG are located in proximity to arterioles in newborn mice. Neuroscience Letters. 2009; 450: 229–234.
14 Tarras-Wahlberg, S., and Rekling, J.C. Hypoglossal motoneurons in newborn mice receive respiratory drive from both sides of the medulla. Neuroscience. 2009; 161: 259–268.
13 Jensen, K.H.R., and Rekling, J.C. Development of a no-wash assay for mitochondrial membrane potential using the styryl dye DASPEI. J. Biomolec. Screen. 2010; 15(9): 1071- 1081.
12 Del Negro, C.A., Hayes, J.A., and Rekling, J.C. Dendritic calcium activity precedes inspiratory bursts in preBötzinger Complex neurons of neonatal mice recorded in vitro. J. Neuroscience. January 19, 2011; 31(3):1017–1022
11 Persson, K., and Rekling, J.C. Population calcium imaging of spontaneous respiratory- and novel motor activity in the facial nucleus and ventral brainstem in newborn mice- J. Physiol. 2011; 589 (10): 2543–2558
10 Lauridsen J.B., Johansen J.L., Rekling J.C., Thirstrup K., Moerk A. and Sager T.N. Regulation of the Bcas1 and Baiap3 transcripts in the subthalamic nucleus in mice recovering from MPTP toxicity. Neurosci Res. 2011; Jul;70(3):269-76.
9 Rekling J.C., Jensen K.H., Jahnsen H. Spontaneous cluster activity in the inferior olivary nucleus in brainstem slices from postnatal mice. J Physiol. 2012; 590 (7): 1547-1562.
8 Albert M, Schmitz SU, Kooistra SM, Malatesta M, Morales Torres C, Rekling JC, Johansen JV, Abarrategui I, Helin K. The Histone Demethylase Jarid1b Ensures Faithful Mouse Development by Protecting Developmental Genes from Aberrant H3K4me3. PLoS Genet. 2013 Apr;9(4):e1003461. doi: 10.1371/journal.pgen.1003461. Epub 2013 Apr 18.
7 Apuschkin, M., Ougaard, M., and Rekling, J.C. Spontaneous calcium waves in granule cells in cerebellar slice cultures. Neuroscience Letters. 2013; 553: 78– 83.
6 Kølvraa M, Müller FC, Jahnsen H, Rekling JC. Mechanisms contributing to cluster formation in the inferior olivary nucleus in brainstem slices from postnatal mice. J Physiol. 2013 Sep 16. [Epub ahead of print].
5 Harsløf, M, Müller, FC, Rohrberg, J., and Rekling, J.C. Fast neuronal labeling in live tissue using a biocytin conjugated fluorescent probe. J. Neuroscience Methods. 2015; 253, 101-109.
4 Phillips WS, Herly M, Del Negro CA, Rekling JC. Organotypic slice cultures containing the prebötzinger complex generate respiratory-like rhythms. J Neurophysiol. 2016 Feb 1;115(2):1063-70.
3 Li, W.C, J.C. Rekling, J.C. Chapter 11 - Electrical Coupling in the Generation of Vertebrate Motor Rhythms, Pages 243-264. Network Functions and Plasticity. Perspectives from Studying Neuronal Electrical Coupling in Microcircuits. Edited by:Jian Jing. Academic Press, 2017. ISBN: 978-0-12-803471-2
2 Mosfeldt Laursen, A., Rekling, J.C. Electrophysiological properties of hypoglossal motoneurones of guinea pigs studied in vitro. Neuroscience. 1989; 30(3): 619-637.
1 Kjoller, E., Rasmussen, C., Rekling J.C. Beta blockers following acute myocardial infarct? Ugeskrift for Laeger. 1987; 149: 50, 3397-3399.
|Camilla Mai Rasmussen||Master student||Rekling lab|
|Carlos Daniel Gomez Martinez||Postdoc||Rekling Lab|
|Esmira Mamedova||PhD student||Rekling lab|
|Henrik Jahnsen||Part-time lecturer||Rekling Lab|
|Jens Christian Rekling||Associate professor||Rekling Lab|
|Morten Bjerre||Laboratory technician||Rekling Lab|