Interactive nanocluster compaction of the ELKS scaffold and Cacophony Ca2+ channels drives sustained active zone potentiation

Research output: Contribution to journal › Journal article › Research › peer-review

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Interactive nanocluster compaction of the ELKS scaffold and Cacophony Ca2+ channels drives sustained active zone potentiation. / Ghelani, Tina; Escher, Marc; Thomas, Ulrich; Esch, Klara; Lützkendorf, Janine; Depner, Harald; Maglione, Marta; Parutto, Pierre; Gratz, Scott; Matkovic-Rachid, Tanja; Ryglewski, Stefanie; Walter, Alexander M.; Holcman, David; O'Connor Giles, Kate; Heine, Martin; Sigrist, Stephan J.

In: Science Advances, Vol. 9, No. 7, eade7804, 2023.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Ghelani, T, Escher, M, Thomas, U, Esch, K, Lützkendorf, J, Depner, H, Maglione, M, Parutto, P, Gratz, S, Matkovic-Rachid, T, Ryglewski, S, Walter, AM, Holcman, D, O'Connor Giles, K, Heine, M & Sigrist, SJ 2023, 'Interactive nanocluster compaction of the ELKS scaffold and Cacophony Ca2+ channels drives sustained active zone potentiation', Science Advances, vol. 9, no. 7, eade7804. https://doi.org/10.1126/sciadv.ade7804

APA

Ghelani, T., Escher, M., Thomas, U., Esch, K., Lützkendorf, J., Depner, H., Maglione, M., Parutto, P., Gratz, S., Matkovic-Rachid, T., Ryglewski, S., Walter, A. M., Holcman, D., O'Connor Giles, K., Heine, M., & Sigrist, S. J. (2023). Interactive nanocluster compaction of the ELKS scaffold and Cacophony Ca2+ channels drives sustained active zone potentiation. Science Advances, 9(7), [eade7804]. https://doi.org/10.1126/sciadv.ade7804

Vancouver

Ghelani T, Escher M, Thomas U, Esch K, Lützkendorf J, Depner H et al. Interactive nanocluster compaction of the ELKS scaffold and Cacophony Ca2+ channels drives sustained active zone potentiation. Science Advances. 2023;9(7). eade7804. https://doi.org/10.1126/sciadv.ade7804

Author

Ghelani, Tina ; Escher, Marc ; Thomas, Ulrich ; Esch, Klara ; Lützkendorf, Janine ; Depner, Harald ; Maglione, Marta ; Parutto, Pierre ; Gratz, Scott ; Matkovic-Rachid, Tanja ; Ryglewski, Stefanie ; Walter, Alexander M. ; Holcman, David ; O'Connor Giles, Kate ; Heine, Martin ; Sigrist, Stephan J. / Interactive nanocluster compaction of the ELKS scaffold and Cacophony Ca2+ channels drives sustained active zone potentiation. In: Science Advances. 2023 ; Vol. 9, No. 7.

Bibtex

@article{936ef537c7cb4e12803de5fa023e6a56,
title = "Interactive nanocluster compaction of the ELKS scaffold and Cacophony Ca2+ channels drives sustained active zone potentiation",
abstract = "At presynaptic active zones (AZs), conserved scaffold protein architectures control synaptic vesicle (SV) release by defining the nanoscale distribution and density of voltage-gated Ca2+ channels (VGCCs). While AZs can potentiate SV release in the minutes range, we lack an understanding of how AZ scaffold components and VGCCs engage into potentiation. We here establish dynamic, intravital single-molecule imaging of endogenously tagged proteins at Drosophila AZs undergoing presynaptic homeostatic potentiation. During potentiation, the numbers of α1 VGCC subunit Cacophony (Cac) increased per AZ, while their mobility decreased and nanoscale distribution compacted. These dynamic Cac changes depended on the interaction between Cac channel's intracellular carboxyl terminus and the membrane-close amino-terminal region of the ELKS-family protein Bruchpilot, whose distribution compacted drastically. The Cac-ELKS/Bruchpilot interaction was also needed for sustained AZ potentiation. Our single-molecule analysis illustrates how the AZ scaffold couples to VGCC nanoscale distribution and dynamics to establish a state of sustained potentiation.",
author = "Tina Ghelani and Marc Escher and Ulrich Thomas and Klara Esch and Janine L{\"u}tzkendorf and Harald Depner and Marta Maglione and Pierre Parutto and Scott Gratz and Tanja Matkovic-Rachid and Stefanie Ryglewski and Walter, {Alexander M.} and David Holcman and {O'Connor Giles}, Kate and Martin Heine and Sigrist, {Stephan J.}",
year = "2023",
doi = "10.1126/sciadv.ade7804",
language = "English",
volume = "9",
journal = "Science advances",
issn = "2375-2548",
publisher = "American Association for the Advancement of Science",
number = "7",

}

RIS

TY - JOUR

T1 - Interactive nanocluster compaction of the ELKS scaffold and Cacophony Ca2+ channels drives sustained active zone potentiation

AU - Ghelani, Tina

AU - Escher, Marc

AU - Thomas, Ulrich

AU - Esch, Klara

AU - Lützkendorf, Janine

AU - Depner, Harald

AU - Maglione, Marta

AU - Parutto, Pierre

AU - Gratz, Scott

AU - Matkovic-Rachid, Tanja

AU - Ryglewski, Stefanie

AU - Walter, Alexander M.

AU - Holcman, David

AU - O'Connor Giles, Kate

AU - Heine, Martin

AU - Sigrist, Stephan J.

PY - 2023

Y1 - 2023

N2 - At presynaptic active zones (AZs), conserved scaffold protein architectures control synaptic vesicle (SV) release by defining the nanoscale distribution and density of voltage-gated Ca2+ channels (VGCCs). While AZs can potentiate SV release in the minutes range, we lack an understanding of how AZ scaffold components and VGCCs engage into potentiation. We here establish dynamic, intravital single-molecule imaging of endogenously tagged proteins at Drosophila AZs undergoing presynaptic homeostatic potentiation. During potentiation, the numbers of α1 VGCC subunit Cacophony (Cac) increased per AZ, while their mobility decreased and nanoscale distribution compacted. These dynamic Cac changes depended on the interaction between Cac channel's intracellular carboxyl terminus and the membrane-close amino-terminal region of the ELKS-family protein Bruchpilot, whose distribution compacted drastically. The Cac-ELKS/Bruchpilot interaction was also needed for sustained AZ potentiation. Our single-molecule analysis illustrates how the AZ scaffold couples to VGCC nanoscale distribution and dynamics to establish a state of sustained potentiation.

AB - At presynaptic active zones (AZs), conserved scaffold protein architectures control synaptic vesicle (SV) release by defining the nanoscale distribution and density of voltage-gated Ca2+ channels (VGCCs). While AZs can potentiate SV release in the minutes range, we lack an understanding of how AZ scaffold components and VGCCs engage into potentiation. We here establish dynamic, intravital single-molecule imaging of endogenously tagged proteins at Drosophila AZs undergoing presynaptic homeostatic potentiation. During potentiation, the numbers of α1 VGCC subunit Cacophony (Cac) increased per AZ, while their mobility decreased and nanoscale distribution compacted. These dynamic Cac changes depended on the interaction between Cac channel's intracellular carboxyl terminus and the membrane-close amino-terminal region of the ELKS-family protein Bruchpilot, whose distribution compacted drastically. The Cac-ELKS/Bruchpilot interaction was also needed for sustained AZ potentiation. Our single-molecule analysis illustrates how the AZ scaffold couples to VGCC nanoscale distribution and dynamics to establish a state of sustained potentiation.

U2 - 10.1126/sciadv.ade7804

DO - 10.1126/sciadv.ade7804

M3 - Journal article

C2 - 36800417

AN - SCOPUS:85148329446

VL - 9

JO - Science advances

JF - Science advances

SN - 2375-2548

IS - 7

M1 - eade7804

ER -

ID: 338823490