Protocol for quantifying pyramidal neuron hyperexcitability in a mouse model of neurodevelopmental encephalopathy

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Protocol for quantifying pyramidal neuron hyperexcitability in a mouse model of neurodevelopmental encephalopathy. / dos Santos, Altair Brito; Larsen, Silas Dalum; Gomez, Carlos Daniel; Sørensen, Jakob Balslev; Perrier, Jean François.

In: STAR Protocols, Vol. 5, No. 2, 102954, 2024.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

dos Santos, AB, Larsen, SD, Gomez, CD, Sørensen, JB & Perrier, JF 2024, 'Protocol for quantifying pyramidal neuron hyperexcitability in a mouse model of neurodevelopmental encephalopathy', STAR Protocols, vol. 5, no. 2, 102954. https://doi.org/10.1016/j.xpro.2024.102954

APA

dos Santos, A. B., Larsen, S. D., Gomez, C. D., Sørensen, J. B., & Perrier, J. F. (2024). Protocol for quantifying pyramidal neuron hyperexcitability in a mouse model of neurodevelopmental encephalopathy. STAR Protocols, 5(2), [102954]. https://doi.org/10.1016/j.xpro.2024.102954

Vancouver

dos Santos AB, Larsen SD, Gomez CD, Sørensen JB, Perrier JF. Protocol for quantifying pyramidal neuron hyperexcitability in a mouse model of neurodevelopmental encephalopathy. STAR Protocols. 2024;5(2). 102954. https://doi.org/10.1016/j.xpro.2024.102954

Author

dos Santos, Altair Brito ; Larsen, Silas Dalum ; Gomez, Carlos Daniel ; Sørensen, Jakob Balslev ; Perrier, Jean François. / Protocol for quantifying pyramidal neuron hyperexcitability in a mouse model of neurodevelopmental encephalopathy. In: STAR Protocols. 2024 ; Vol. 5, No. 2.

Bibtex

@article{dcefdae0721e458aa9ecfe78db43ac25,
title = "Protocol for quantifying pyramidal neuron hyperexcitability in a mouse model of neurodevelopmental encephalopathy",
abstract = "Here, we present a protocol for quantifying pyramidal neuron hyperexcitability in a mouse model of STXBP1 neurodevelopmental encephalopathy (Stxbp1hap). We describe steps for preparing brain slices, positioning electrodes, and performing an excitability test to investigate microcircuit failures. This protocol is based on recording layer 2/3 cortical pyramidal neurons in response to stimulation of two independent sets of excitatory axons that recruit feedforward inhibition microcircuits. For complete details on the use and execution of this protocol, please refer to Dos Santos et al.1",
keywords = "Genetics, Health Sciences, Neuroscience",
author = "{dos Santos}, {Altair Brito} and Larsen, {Silas Dalum} and Gomez, {Carlos Daniel} and S{\o}rensen, {Jakob Balslev} and Perrier, {Jean Fran{\c c}ois}",
note = "Publisher Copyright: {\textcopyright} 2024 The Authors",
year = "2024",
doi = "10.1016/j.xpro.2024.102954",
language = "English",
volume = "5",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "2",

}

RIS

TY - JOUR

T1 - Protocol for quantifying pyramidal neuron hyperexcitability in a mouse model of neurodevelopmental encephalopathy

AU - dos Santos, Altair Brito

AU - Larsen, Silas Dalum

AU - Gomez, Carlos Daniel

AU - Sørensen, Jakob Balslev

AU - Perrier, Jean François

N1 - Publisher Copyright: © 2024 The Authors

PY - 2024

Y1 - 2024

N2 - Here, we present a protocol for quantifying pyramidal neuron hyperexcitability in a mouse model of STXBP1 neurodevelopmental encephalopathy (Stxbp1hap). We describe steps for preparing brain slices, positioning electrodes, and performing an excitability test to investigate microcircuit failures. This protocol is based on recording layer 2/3 cortical pyramidal neurons in response to stimulation of two independent sets of excitatory axons that recruit feedforward inhibition microcircuits. For complete details on the use and execution of this protocol, please refer to Dos Santos et al.1

AB - Here, we present a protocol for quantifying pyramidal neuron hyperexcitability in a mouse model of STXBP1 neurodevelopmental encephalopathy (Stxbp1hap). We describe steps for preparing brain slices, positioning electrodes, and performing an excitability test to investigate microcircuit failures. This protocol is based on recording layer 2/3 cortical pyramidal neurons in response to stimulation of two independent sets of excitatory axons that recruit feedforward inhibition microcircuits. For complete details on the use and execution of this protocol, please refer to Dos Santos et al.1

KW - Genetics

KW - Health Sciences

KW - Neuroscience

U2 - 10.1016/j.xpro.2024.102954

DO - 10.1016/j.xpro.2024.102954

M3 - Journal article

C2 - 38492227

AN - SCOPUS:85188189040

VL - 5

JO - STAR Protocols

JF - STAR Protocols

SN - 2666-1667

IS - 2

M1 - 102954

ER -

ID: 387269165