An Improved Method for Growing Primary Neurons on Electron Microscopy Grids Co-Cultured with Astrocytes

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

An Improved Method for Growing Primary Neurons on Electron Microscopy Grids Co-Cultured with Astrocytes. / Kumar, Ishika; Paudyal, Anju; Kádková, Anna; Stewart, Michelle; Sørensen, Jakob Balslev; Radecke, Julika.

In: International Journal of Molecular Sciences, Vol. 24, No. 20, 15191, 2023.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Kumar, I, Paudyal, A, Kádková, A, Stewart, M, Sørensen, JB & Radecke, J 2023, 'An Improved Method for Growing Primary Neurons on Electron Microscopy Grids Co-Cultured with Astrocytes', International Journal of Molecular Sciences, vol. 24, no. 20, 15191. https://doi.org/10.3390/ijms242015191

APA

Kumar, I., Paudyal, A., Kádková, A., Stewart, M., Sørensen, J. B., & Radecke, J. (2023). An Improved Method for Growing Primary Neurons on Electron Microscopy Grids Co-Cultured with Astrocytes. International Journal of Molecular Sciences, 24(20), [15191]. https://doi.org/10.3390/ijms242015191

Vancouver

Kumar I, Paudyal A, Kádková A, Stewart M, Sørensen JB, Radecke J. An Improved Method for Growing Primary Neurons on Electron Microscopy Grids Co-Cultured with Astrocytes. International Journal of Molecular Sciences. 2023;24(20). 15191. https://doi.org/10.3390/ijms242015191

Author

Kumar, Ishika ; Paudyal, Anju ; Kádková, Anna ; Stewart, Michelle ; Sørensen, Jakob Balslev ; Radecke, Julika. / An Improved Method for Growing Primary Neurons on Electron Microscopy Grids Co-Cultured with Astrocytes. In: International Journal of Molecular Sciences. 2023 ; Vol. 24, No. 20.

Bibtex

@article{651e7a6a3fc445d9b22bab4d29a4c12c,

title = "An Improved Method for Growing Primary Neurons on Electron Microscopy Grids Co-Cultured with Astrocytes",

abstract = "With the increasing popularity of cryo-electron tomography (cryo-ET) in recent years, the quest to establish a method for growing primary neurons directly on electron microscopy grids (EM grids) has been ongoing. Here we describe a straightforward way to establish a mature neuronal network on EM grids, which includes formation of synaptic contacts. These synapses were thin enough to allow for direct visualization of small filaments such as SNARE proteins tethering the synaptic vesicle (SV) to the active zone plasma membrane on a Titan Krios without prior focused ion-beam milling.",

keywords = "astrocytes, cryo-electron microscopy, primary neurons, synapse",

author = "Ishika Kumar and Anju Paudyal and Anna K{\'a}dkov{\'a} and Michelle Stewart and S{\o}rensen, {Jakob Balslev} and Julika Radecke",

note = "Publisher Copyright: {\textcopyright} 2023 by the authors.",

year = "2023",

doi = "10.3390/ijms242015191",

language = "English",

volume = "24",

journal = "International Journal of Molecular Sciences (Online)",

issn = "1661-6596",

publisher = "MDPI AG",

number = "20",

}

RIS

TY - JOUR

T1 - An Improved Method for Growing Primary Neurons on Electron Microscopy Grids Co-Cultured with Astrocytes

AU - Kumar, Ishika

AU - Paudyal, Anju

AU - Kádková, Anna

AU - Stewart, Michelle

AU - Sørensen, Jakob Balslev

AU - Radecke, Julika

PY - 2023

Y1 - 2023

N2 - With the increasing popularity of cryo-electron tomography (cryo-ET) in recent years, the quest to establish a method for growing primary neurons directly on electron microscopy grids (EM grids) has been ongoing. Here we describe a straightforward way to establish a mature neuronal network on EM grids, which includes formation of synaptic contacts. These synapses were thin enough to allow for direct visualization of small filaments such as SNARE proteins tethering the synaptic vesicle (SV) to the active zone plasma membrane on a Titan Krios without prior focused ion-beam milling.

AB - With the increasing popularity of cryo-electron tomography (cryo-ET) in recent years, the quest to establish a method for growing primary neurons directly on electron microscopy grids (EM grids) has been ongoing. Here we describe a straightforward way to establish a mature neuronal network on EM grids, which includes formation of synaptic contacts. These synapses were thin enough to allow for direct visualization of small filaments such as SNARE proteins tethering the synaptic vesicle (SV) to the active zone plasma membrane on a Titan Krios without prior focused ion-beam milling.

KW - astrocytes

KW - cryo-electron microscopy

KW - primary neurons

KW - synapse

U2 - 10.3390/ijms242015191

DO - 10.3390/ijms242015191

M3 - Journal article

C2 - 37894872

AN - SCOPUS:85175275709

VL - 24

JO - International Journal of Molecular Sciences (Online)

JF - International Journal of Molecular Sciences (Online)

SN - 1661-6596

IS - 20

M1 - 15191

ER -

ID: 372327571

Department of Neuroscience