Coupling of Organotypic Brain Slice Cultures to Silicon-Based Arrays of Electrodes

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Coupling of Organotypic Brain Slice Cultures to Silicon-Based Arrays of Electrodes. / Jahnsen, Henrik; Kristensen, Bjarne Winther; Thiébaud, Pierre; Noraberg, Jens; Jakobsen, Birthe; Bove, Marco; Martinoa, Sergio; Koudelka-Hep, Milena; Grattarola, Massimo; Zimmer, Jens.

In: Methods, Vol. 18, No. 2, 1999, p. 160-172.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Jahnsen, H, Kristensen, BW, Thiébaud, P, Noraberg, J, Jakobsen, B, Bove, M, Martinoa, S, Koudelka-Hep, M, Grattarola, M & Zimmer, J 1999, 'Coupling of Organotypic Brain Slice Cultures to Silicon-Based Arrays of Electrodes', Methods, vol. 18, no. 2, pp. 160-172. https://doi.org/10.1006/meth.1999.0769

APA

Jahnsen, H., Kristensen, B. W., Thiébaud, P., Noraberg, J., Jakobsen, B., Bove, M., Martinoa, S., Koudelka-Hep, M., Grattarola, M., & Zimmer, J. (1999). Coupling of Organotypic Brain Slice Cultures to Silicon-Based Arrays of Electrodes. Methods, 18(2), 160-172. https://doi.org/10.1006/meth.1999.0769

Vancouver

Jahnsen H, Kristensen BW, Thiébaud P, Noraberg J, Jakobsen B, Bove M et al. Coupling of Organotypic Brain Slice Cultures to Silicon-Based Arrays of Electrodes. Methods. 1999;18(2):160-172. https://doi.org/10.1006/meth.1999.0769

Author

Jahnsen, Henrik ; Kristensen, Bjarne Winther ; Thiébaud, Pierre ; Noraberg, Jens ; Jakobsen, Birthe ; Bove, Marco ; Martinoa, Sergio ; Koudelka-Hep, Milena ; Grattarola, Massimo ; Zimmer, Jens. / Coupling of Organotypic Brain Slice Cultures to Silicon-Based Arrays of Electrodes. In: Methods. 1999 ; Vol. 18, No. 2. pp. 160-172.

Bibtex

@article{f317dc3074c811dbbee902004c4f4f50,
title = "Coupling of Organotypic Brain Slice Cultures to Silicon-Based Arrays of Electrodes",
abstract = "Fetal or early postnatal brain tissue can be cultured in viable and healthy condition for several weeks with development and preservation of the basic cellular and connective organization as so-called organotypic brain slice cultures. Here we demonstrate and describe how it is possible to establish such hippocampal rat brain slice cultures on biocompatible silicon-based chips with arrays of electrodes with a histological organization comparable to that of conventional brain slice cultures grown by the roller drum technique and on semiporous membranes. Intracellular and extracellular recordings from neurons in the slice cultures show that the electroresponsive properties of the neurons and synaptic circuitry are in accordance with those described for cells in acutely prepared slices of the adult rat hippocampus. Based on the recordings and the possibilities of stimulating the cultured cells through the electrode arrays it is anticipated that the setup eventually will allow long-term studies of defined neuronal networks and provide valuable information on both normal and neurotoxicological and neuropathological conditions.",
author = "Henrik Jahnsen and Kristensen, {Bjarne Winther} and Pierre Thi{\'e}baud and Jens Noraberg and Birthe Jakobsen and Marco Bove and Sergio Martinoa and Milena Koudelka-Hep and Massimo Grattarola and Jens Zimmer",
year = "1999",
doi = "10.1006/meth.1999.0769",
language = "English",
volume = "18",
pages = "160--172",
journal = "Methods",
issn = "1046-2023",
publisher = "Academic Press",
number = "2",

}

RIS

TY - JOUR

T1 - Coupling of Organotypic Brain Slice Cultures to Silicon-Based Arrays of Electrodes

AU - Jahnsen, Henrik

AU - Kristensen, Bjarne Winther

AU - Thiébaud, Pierre

AU - Noraberg, Jens

AU - Jakobsen, Birthe

AU - Bove, Marco

AU - Martinoa, Sergio

AU - Koudelka-Hep, Milena

AU - Grattarola, Massimo

AU - Zimmer, Jens

PY - 1999

Y1 - 1999

N2 - Fetal or early postnatal brain tissue can be cultured in viable and healthy condition for several weeks with development and preservation of the basic cellular and connective organization as so-called organotypic brain slice cultures. Here we demonstrate and describe how it is possible to establish such hippocampal rat brain slice cultures on biocompatible silicon-based chips with arrays of electrodes with a histological organization comparable to that of conventional brain slice cultures grown by the roller drum technique and on semiporous membranes. Intracellular and extracellular recordings from neurons in the slice cultures show that the electroresponsive properties of the neurons and synaptic circuitry are in accordance with those described for cells in acutely prepared slices of the adult rat hippocampus. Based on the recordings and the possibilities of stimulating the cultured cells through the electrode arrays it is anticipated that the setup eventually will allow long-term studies of defined neuronal networks and provide valuable information on both normal and neurotoxicological and neuropathological conditions.

AB - Fetal or early postnatal brain tissue can be cultured in viable and healthy condition for several weeks with development and preservation of the basic cellular and connective organization as so-called organotypic brain slice cultures. Here we demonstrate and describe how it is possible to establish such hippocampal rat brain slice cultures on biocompatible silicon-based chips with arrays of electrodes with a histological organization comparable to that of conventional brain slice cultures grown by the roller drum technique and on semiporous membranes. Intracellular and extracellular recordings from neurons in the slice cultures show that the electroresponsive properties of the neurons and synaptic circuitry are in accordance with those described for cells in acutely prepared slices of the adult rat hippocampus. Based on the recordings and the possibilities of stimulating the cultured cells through the electrode arrays it is anticipated that the setup eventually will allow long-term studies of defined neuronal networks and provide valuable information on both normal and neurotoxicological and neuropathological conditions.

U2 - 10.1006/meth.1999.0769

DO - 10.1006/meth.1999.0769

M3 - Journal article

VL - 18

SP - 160

EP - 172

JO - Methods

JF - Methods

SN - 1046-2023

IS - 2

ER -

ID: 190778