Characterization of the in vitro propagation of epileptiform electrophysiological activity in organotypic hippocampal slice cultures coupled to 3D microelectrode arrays
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Characterization of the in vitro propagation of epileptiform electrophysiological activity in organotypic hippocampal slice cultures coupled to 3D microelectrode arrays. / Pisciotta, Marzia ; Morgavi, Giovanna; Jahnsen, Henrik.
In: Brain Research, Vol. 1358, 28.10.2010, p. 46-53.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - Characterization of the in vitro propagation of epileptiform electrophysiological activity in organotypic hippocampal slice cultures coupled to 3D microelectrode arrays
AU - Pisciotta, Marzia
AU - Morgavi, Giovanna
AU - Jahnsen, Henrik
PY - 2010/10/28
Y1 - 2010/10/28
N2 - Dynamic aspects of the propagation of epileptiform activity have so far received little attention. With the aim of providing new insights about the spatial features of the propagation of epileptic seizures in the nervous system, we studied in vitro the initiation and propagation of traveling epileptiform waves of electrophysiological activity in the hippocampus by means of substrate three-dimensional microelectrode arrays (MEAs) for extracellular measurements. Pharmacologically disinhibited hippocampal slices spontaneously generate epileptiform bursts mostly originating in CA3 and propagating to CA1. Our study specifically addressed the activity-dependent changes of the propagation of traveling electrophysiological waves in organotypic hippocampal slices during epileptiform discharge and in particular our question is: what happens to the epileptic signals during their propagation through the slice? Multichannel data analysis enabled us to quantify an activity-dependent increase in the propagation velocity of spontaneous bursts. Moreover, through the evaluation of the coherence of the signals, it was possible to point out that only the lower-frequency components (<95Hz) of the electrical activity are completely coherent with respect to the activity originating in the CA3, while components at higher frequencies lose the coherence, possibly suggesting that the cellular mechanism mediating propagation of electrophysiological activity becomes ineffective for those firing rates exceeding an upper bound or that some noise of neuronal origin was added to the signal during propagation.Copyright © 2010 Elsevier B.V. All rights reserved.PMID:20713026[PubMed - indexed for MEDLINE] Publication Types, MeSH Terms, SubstancesPublication TypesIn VitroResearch Support, Non-U.S. Gov'tMeSH TermsAnimalsAnimals, NewbornConvulsants/pharmacologyElectric Stimulation/methodsElectrophysiological Phenomena/drug effectsElectrophysiological Phenomena/physiology*Evoked Potentials/drug effectsEvoked Potentials/physiology*Hippocampus/anatomy & histologyHippocampus/drug effectsHippocampus/physiology*Microelectrodes*Organ Culture TechniquesPicrotoxin/pharmacologyRatsRats, WistarReaction Time/drug effectsReaction Time/physiologyTime FactorsSubstancesConvulsantsPicrotoxinLinkOut - more resourcesFull Text SourcesElsevier ScienceEBSCOOhioLINK Electronic Journal CenterSwets Information ServicesMolecular Biology DatabasesPICROTOXIN - HSDBLibrariesLinkOut Holdings •Supplemental Content Related citationsDecrease in synaptic transmission can reverse the propagation direction of epileptiform activity in hippocampus in vivo. [J Neurophysiol. 2005] Decrease in synaptic transmission can reverse the propagation direction of epileptiform activity in hippocampus in vivo.Feng Z, Durand DM. J Neurophysiol. 2005 Mar; 93(3):1158-64. Epub 2004 Oct 20.Chloride-cotransport blockade desynchronizes neuronal discharge in the "epileptic" hippocampal slice. [J Neurophysiol. 2000] Chloride-cotransport blockade desynchronizes neuronal discharge in the "epileptic" hippocampal slice.Hochman DW, Schwartzkroin PA. J Neurophysiol. 2000 Jan; 83(1):406-17. Propagation of low calcium non-synaptic induced epileptiform activity to the contralateral hippocampus in vivo. [Brain Res. 2005] Propagation of low calcium non-synaptic induced epileptiform activity to the contralateral hippocampus in vivo.Feng Z, Durand DM. Brain Res. 2005 Sep 7; 1055(1-2):25-35. Endogenous nitric oxide is a key promoting factor for initiation of seizure-like events in hippocampal and entorhinal cortex slices. [J Neurosci. 2009] Endogenous nitric oxide is a key promoting factor for initiation of seizure-like events in hippocampal and entorhinal cortex slices.Kovács R, Rabanus A, Otáhal J, Patzak A, Kardos J, Albus K, Heinemann U, Kann O. J Neurosci. 2009 Jul 1; 29(26):8565-77. Epileptiform activity induced by pilocarpine in the rat hippocampal-entorhinal slice preparation. [Neuroscience. 1996] Epileptiform activity induced by pilocarpine in the rat hippocampal-entorhinal slice preparation.Nagao T, Alonso A, Avoli M. Neuroscience. 1996 May; 72(2):399-408. See reviews... See all... Related informationRelated Citations Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
AB - Dynamic aspects of the propagation of epileptiform activity have so far received little attention. With the aim of providing new insights about the spatial features of the propagation of epileptic seizures in the nervous system, we studied in vitro the initiation and propagation of traveling epileptiform waves of electrophysiological activity in the hippocampus by means of substrate three-dimensional microelectrode arrays (MEAs) for extracellular measurements. Pharmacologically disinhibited hippocampal slices spontaneously generate epileptiform bursts mostly originating in CA3 and propagating to CA1. Our study specifically addressed the activity-dependent changes of the propagation of traveling electrophysiological waves in organotypic hippocampal slices during epileptiform discharge and in particular our question is: what happens to the epileptic signals during their propagation through the slice? Multichannel data analysis enabled us to quantify an activity-dependent increase in the propagation velocity of spontaneous bursts. Moreover, through the evaluation of the coherence of the signals, it was possible to point out that only the lower-frequency components (<95Hz) of the electrical activity are completely coherent with respect to the activity originating in the CA3, while components at higher frequencies lose the coherence, possibly suggesting that the cellular mechanism mediating propagation of electrophysiological activity becomes ineffective for those firing rates exceeding an upper bound or that some noise of neuronal origin was added to the signal during propagation.Copyright © 2010 Elsevier B.V. All rights reserved.PMID:20713026[PubMed - indexed for MEDLINE] Publication Types, MeSH Terms, SubstancesPublication TypesIn VitroResearch Support, Non-U.S. Gov'tMeSH TermsAnimalsAnimals, NewbornConvulsants/pharmacologyElectric Stimulation/methodsElectrophysiological Phenomena/drug effectsElectrophysiological Phenomena/physiology*Evoked Potentials/drug effectsEvoked Potentials/physiology*Hippocampus/anatomy & histologyHippocampus/drug effectsHippocampus/physiology*Microelectrodes*Organ Culture TechniquesPicrotoxin/pharmacologyRatsRats, WistarReaction Time/drug effectsReaction Time/physiologyTime FactorsSubstancesConvulsantsPicrotoxinLinkOut - more resourcesFull Text SourcesElsevier ScienceEBSCOOhioLINK Electronic Journal CenterSwets Information ServicesMolecular Biology DatabasesPICROTOXIN - HSDBLibrariesLinkOut Holdings •Supplemental Content Related citationsDecrease in synaptic transmission can reverse the propagation direction of epileptiform activity in hippocampus in vivo. [J Neurophysiol. 2005] Decrease in synaptic transmission can reverse the propagation direction of epileptiform activity in hippocampus in vivo.Feng Z, Durand DM. J Neurophysiol. 2005 Mar; 93(3):1158-64. Epub 2004 Oct 20.Chloride-cotransport blockade desynchronizes neuronal discharge in the "epileptic" hippocampal slice. [J Neurophysiol. 2000] Chloride-cotransport blockade desynchronizes neuronal discharge in the "epileptic" hippocampal slice.Hochman DW, Schwartzkroin PA. J Neurophysiol. 2000 Jan; 83(1):406-17. Propagation of low calcium non-synaptic induced epileptiform activity to the contralateral hippocampus in vivo. [Brain Res. 2005] Propagation of low calcium non-synaptic induced epileptiform activity to the contralateral hippocampus in vivo.Feng Z, Durand DM. Brain Res. 2005 Sep 7; 1055(1-2):25-35. Endogenous nitric oxide is a key promoting factor for initiation of seizure-like events in hippocampal and entorhinal cortex slices. [J Neurosci. 2009] Endogenous nitric oxide is a key promoting factor for initiation of seizure-like events in hippocampal and entorhinal cortex slices.Kovács R, Rabanus A, Otáhal J, Patzak A, Kardos J, Albus K, Heinemann U, Kann O. J Neurosci. 2009 Jul 1; 29(26):8565-77. Epileptiform activity induced by pilocarpine in the rat hippocampal-entorhinal slice preparation. [Neuroscience. 1996] Epileptiform activity induced by pilocarpine in the rat hippocampal-entorhinal slice preparation.Nagao T, Alonso A, Avoli M. Neuroscience. 1996 May; 72(2):399-408. See reviews... See all... Related informationRelated Citations Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
U2 - 10.1016/j.brainres.2010.08.028
DO - 10.1016/j.brainres.2010.08.028
M3 - Journal article
C2 - 20713026
VL - 1358
SP - 46
EP - 53
JO - Brain Research
JF - Brain Research
SN - 0006-8993
ER -
ID: 33754347