Dendrite-derived supernumerary axons on adult axotomized motor neurons possess proteins that are essential for the initiation and propagation of action potentials and synaptic vesicle release
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Dendrite-derived supernumerary axons on adult axotomized motor neurons possess proteins that are essential for the initiation and propagation of action potentials and synaptic vesicle release. / Meehan, Claire Francesca; MacDermid, Victoria E; Montague, Steven J; Neuber-Hess, Monica; Rose, P Ken.
In: Journal of Neuroscience, Vol. 31, No. 18, 2011, p. 6732-40.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Dendrite-derived supernumerary axons on adult axotomized motor neurons possess proteins that are essential for the initiation and propagation of action potentials and synaptic vesicle release
AU - Meehan, Claire Francesca
AU - MacDermid, Victoria E
AU - Montague, Steven J
AU - Neuber-Hess, Monica
AU - Rose, P Ken
PY - 2011
Y1 - 2011
N2 - Axotomy can trigger profound alterations in the neuronal polarity of adult neurons in vivo. This can manifest itself in the development of new axon-like processes emanating from the tips of distal dendrites. Previously, these processes have been defined as axonal based on their axonal morphology. This study extends this definition to determine whether, more importantly, these processes possess the prerequisite molecular machinery to function as axons. Using a combination of intracellular labeling and immunohistochemistry, we demonstrate that the distribution of voltage-gated sodium channels on these processes matches the arrangement of these channels that is necessary for the initiation and conduction of action potentials. At terminal bouton-like structures they possess key proteins necessary for the release of synaptic vesicles (SV2 and synaptophysin). Thus, axon-like processes emanating from the tips of distal dendrites represent a rearrangement of neuronal polarity whereby axotomized neurons can develop additional functional axons in vivo.
AB - Axotomy can trigger profound alterations in the neuronal polarity of adult neurons in vivo. This can manifest itself in the development of new axon-like processes emanating from the tips of distal dendrites. Previously, these processes have been defined as axonal based on their axonal morphology. This study extends this definition to determine whether, more importantly, these processes possess the prerequisite molecular machinery to function as axons. Using a combination of intracellular labeling and immunohistochemistry, we demonstrate that the distribution of voltage-gated sodium channels on these processes matches the arrangement of these channels that is necessary for the initiation and conduction of action potentials. At terminal bouton-like structures they possess key proteins necessary for the release of synaptic vesicles (SV2 and synaptophysin). Thus, axon-like processes emanating from the tips of distal dendrites represent a rearrangement of neuronal polarity whereby axotomized neurons can develop additional functional axons in vivo.
KW - Action Potentials
KW - Animals
KW - Axons
KW - Axotomy
KW - Cats
KW - Dendrites
KW - Immunohistochemistry
KW - Motor Neurons
KW - Sodium Channels
KW - Synapses
KW - Synaptic Transmission
KW - Synaptic Vesicles
KW - Synaptophysin
U2 - 10.1523/JNEUROSCI.5377-10.2011
DO - 10.1523/JNEUROSCI.5377-10.2011
M3 - Journal article
C2 - 21543602
VL - 31
SP - 6732
EP - 6740
JO - The Journal of neuroscience : the official journal of the Society for Neuroscience
JF - The Journal of neuroscience : the official journal of the Society for Neuroscience
SN - 0270-6474
IS - 18
ER -
ID: 40314797