Ventricular CSF proteomic profiles and predictors of surgical treatment outcome in chronic hydrocephalus
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Ventricular CSF proteomic profiles and predictors of surgical treatment outcome in chronic hydrocephalus. / Rostgaard, Nina; Olsen, Markus Harboe; Lolansen, Sara Diana; Nørager, Nicolas Hernandez; Plomgaard, Peter; MacAulay, Nanna; Juhler, Marianne.
In: Acta Neurochirurgica, Vol. 165, 2023, p. 4059–4070.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Ventricular CSF proteomic profiles and predictors of surgical treatment outcome in chronic hydrocephalus
AU - Rostgaard, Nina
AU - Olsen, Markus Harboe
AU - Lolansen, Sara Diana
AU - Nørager, Nicolas Hernandez
AU - Plomgaard, Peter
AU - MacAulay, Nanna
AU - Juhler, Marianne
N1 - Publisher Copyright: © 2023, The Author(s).
PY - 2023
Y1 - 2023
N2 - Background: By applying an unbiased proteomic approach, we aimed to search for cerebrospinal fluid (CSF) protein biomarkers distinguishing between obstructive and communicating hydrocephalus in order to improve appropriate surgical selection for endoscopic third ventriculostomy vs. shunt implants. Our second study purpose was to look for potential CSF biomarkers distinguishing between patients with adult chronic hydrocephalus benefitting from surgery (responders) vs. those who did not (non-responders). Methods: Ventricular CSF samples were collected from 62 patients with communicating hydrocephalus and 28 patients with obstructive hydrocephalus. CSF was collected in relation to the patients’ surgical treatment. As a control group, CSF was collected from ten patients with unruptured aneurysm undergoing preventive surgery (vascular clipping). Results: Mass spectrometry-based proteomic analysis of the samples identified 1251 unique proteins. No proteins differed significantly between the communicating hydrocephalus group and the obstructive hydrocephalus group. Four proteins were found to be significantly less abundant in CSF from communicating hydrocephalus patients compared to control subjects. A PCA plot revealed similar proteomic CSF profiles of obstructive and communicating hydrocephalus and control samples. For obstructive hydrocephalus, ten proteins were found to predict responders from non-responders. Conclusion: Here, we show that the proteomic profile of ventricular CSF from patients with hydrocephalus differs slightly from control subjects. Furthermore, we find ten predictors of response to surgical outcome (endoscopic third ventriculostomy or ventriculo-peritoneal shunt) in patients with obstructive hydrocephalus.
AB - Background: By applying an unbiased proteomic approach, we aimed to search for cerebrospinal fluid (CSF) protein biomarkers distinguishing between obstructive and communicating hydrocephalus in order to improve appropriate surgical selection for endoscopic third ventriculostomy vs. shunt implants. Our second study purpose was to look for potential CSF biomarkers distinguishing between patients with adult chronic hydrocephalus benefitting from surgery (responders) vs. those who did not (non-responders). Methods: Ventricular CSF samples were collected from 62 patients with communicating hydrocephalus and 28 patients with obstructive hydrocephalus. CSF was collected in relation to the patients’ surgical treatment. As a control group, CSF was collected from ten patients with unruptured aneurysm undergoing preventive surgery (vascular clipping). Results: Mass spectrometry-based proteomic analysis of the samples identified 1251 unique proteins. No proteins differed significantly between the communicating hydrocephalus group and the obstructive hydrocephalus group. Four proteins were found to be significantly less abundant in CSF from communicating hydrocephalus patients compared to control subjects. A PCA plot revealed similar proteomic CSF profiles of obstructive and communicating hydrocephalus and control samples. For obstructive hydrocephalus, ten proteins were found to predict responders from non-responders. Conclusion: Here, we show that the proteomic profile of ventricular CSF from patients with hydrocephalus differs slightly from control subjects. Furthermore, we find ten predictors of response to surgical outcome (endoscopic third ventriculostomy or ventriculo-peritoneal shunt) in patients with obstructive hydrocephalus.
KW - Biomarkers
KW - Cerebrospinal fluid
KW - Idiopathic normal pressure hydrocephalus
KW - Mass spectrometry
KW - Obstructive hydrocephalus
KW - Predictors
KW - Proteomic profile
KW - Proteomics
U2 - 10.1007/s00701-023-05832-y
DO - 10.1007/s00701-023-05832-y
M3 - Journal article
C2 - 37857909
AN - SCOPUS:85174513387
VL - 165
SP - 4059
EP - 4070
JO - Acta Neurochirurgica
JF - Acta Neurochirurgica
SN - 0001-6268
ER -
ID: 371932012