Steroid-based amphiphiles for membrane protein study: The importance of alkyl spacers for protein stability
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Steroid-based amphiphiles for membrane protein study : The importance of alkyl spacers for protein stability. / Ehsan, Muhammad; Das, Manabendra; Stern, Valerie; Du, Yang; Mortensen, Jonas S.; Hariharan, Parameswaran; Byrne, Bernadette; Loland, Claus J.; Kobilka, Brian K.; Guan, Lan; Chae, Pil Seok.
In: ChemBioChem, Vol. 19, No. 13, 2018, p. 1433-1443.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Steroid-based amphiphiles for membrane protein study
T2 - The importance of alkyl spacers for protein stability
AU - Ehsan, Muhammad
AU - Das, Manabendra
AU - Stern, Valerie
AU - Du, Yang
AU - Mortensen, Jonas S.
AU - Hariharan, Parameswaran
AU - Byrne, Bernadette
AU - Loland, Claus J.
AU - Kobilka, Brian K.
AU - Guan, Lan
AU - Chae, Pil Seok
PY - 2018
Y1 - 2018
N2 - Membrane proteins allow effective communication between cells and organelles and their external environments. Maintaining membrane protein stability in a non-native environment is the major bottleneck to their structural study. Detergents are widely used to extract membrane proteins from the membrane and to keep the extracted protein in a stable state for downstream characterisation. In this study, three sets of steroid-based amphiphiles—glyco-diosgenin analogues (GDNs) and steroid-based pentasaccharides either lacking a linker (SPSs) or containing a linker (SPS-Ls)—have been developed as new chemical tools for membrane protein research. These detergents were tested with three membrane proteins in order to characterise their ability to extract membrane proteins from the membrane and to stabilise membrane proteins long-term. Some of the detergents, particularly the SPS-Ls, displayed favourable behaviour with the tested membrane proteins. This result indicates the potential utility of these detergents as chemical tools for membrane protein structural study and a critical role of the simple alkyl spacer in determining detergent efficacy.
AB - Membrane proteins allow effective communication between cells and organelles and their external environments. Maintaining membrane protein stability in a non-native environment is the major bottleneck to their structural study. Detergents are widely used to extract membrane proteins from the membrane and to keep the extracted protein in a stable state for downstream characterisation. In this study, three sets of steroid-based amphiphiles—glyco-diosgenin analogues (GDNs) and steroid-based pentasaccharides either lacking a linker (SPSs) or containing a linker (SPS-Ls)—have been developed as new chemical tools for membrane protein research. These detergents were tested with three membrane proteins in order to characterise their ability to extract membrane proteins from the membrane and to stabilise membrane proteins long-term. Some of the detergents, particularly the SPS-Ls, displayed favourable behaviour with the tested membrane proteins. This result indicates the potential utility of these detergents as chemical tools for membrane protein structural study and a critical role of the simple alkyl spacer in determining detergent efficacy.
KW - Amphiphiles
KW - Membrane proteins
KW - Molecular design
KW - Protein stability
KW - Steroids
U2 - 10.1002/cbic.201800106
DO - 10.1002/cbic.201800106
M3 - Journal article
C2 - 29660780
AN - SCOPUS:85047747915
VL - 19
SP - 1433
EP - 1443
JO - ChemBioChem
JF - ChemBioChem
SN - 1439-4227
IS - 13
ER -
ID: 209804756