Dendronic trimaltoside amphiphiles (DTMs) for membrane protein study
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Dendronic trimaltoside amphiphiles (DTMs) for membrane protein study. / Sadaf, Aiman; Du, Yang; Santillan, Claudia; Mortensen, Jonas S.; Molist, Iago; Seven, Alpay B.; Hariharan, Parameswaran; Skiniotis, Georgios; Loland, Claus J.; Kobilka, Brian K.; Guan, Lan; Byrne, Bernadette; Chae, Pil Seok.
In: Chemical Science, Vol. 8, No. 12, 2017, p. 8315-8324.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Dendronic trimaltoside amphiphiles (DTMs) for membrane protein study
AU - Sadaf, Aiman
AU - Du, Yang
AU - Santillan, Claudia
AU - Mortensen, Jonas S.
AU - Molist, Iago
AU - Seven, Alpay B.
AU - Hariharan, Parameswaran
AU - Skiniotis, Georgios
AU - Loland, Claus J.
AU - Kobilka, Brian K.
AU - Guan, Lan
AU - Byrne, Bernadette
AU - Chae, Pil Seok
PY - 2017
Y1 - 2017
N2 - The critical contribution of membrane proteins in normal cellular function makes their detailed structure and functional analysis essential. Detergents, amphipathic agents with the ability to maintain membrane proteins in a soluble state in aqueous solution, have key roles in membrane protein manipulation. Structural and functional stability is a prerequisite for biophysical characterization. However, many conventional detergents are limited in their ability to stabilize membrane proteins, making development of novel detergents for membrane protein manipulation an important research area. The architecture of a detergent hydrophobic group, that directly interacts with the hydrophobic segment of membrane proteins, is a key factor in dictating their efficacy for both membrane protein solubilization and stabilization. In the current study, we developed two sets of maltoside-based detergents with four alkyl chains by introducing dendronic hydrophobic groups connected to a trimaltoside head group, designated dendronic trimaltosides (DTMs). Representative DTMs conferred enhanced stabilization to multiple membrane proteins compared to the benchmark conventional detergent, DDM. One DTM (i.e., DTM-A6) clearly outperformed DDM in stabilizing human β2 adrenergic receptor (β2AR) and its complex with Gs protein. A further evaluation of this DTM led to a clear visualization of β2AR-Gs complex via electron microscopic analysis. Thus, the current study not only provides novel detergent tools useful for membrane protein study, but also suggests that the dendronic architecture has a role in governing detergent efficacy for membrane protein stabilization.
AB - The critical contribution of membrane proteins in normal cellular function makes their detailed structure and functional analysis essential. Detergents, amphipathic agents with the ability to maintain membrane proteins in a soluble state in aqueous solution, have key roles in membrane protein manipulation. Structural and functional stability is a prerequisite for biophysical characterization. However, many conventional detergents are limited in their ability to stabilize membrane proteins, making development of novel detergents for membrane protein manipulation an important research area. The architecture of a detergent hydrophobic group, that directly interacts with the hydrophobic segment of membrane proteins, is a key factor in dictating their efficacy for both membrane protein solubilization and stabilization. In the current study, we developed two sets of maltoside-based detergents with four alkyl chains by introducing dendronic hydrophobic groups connected to a trimaltoside head group, designated dendronic trimaltosides (DTMs). Representative DTMs conferred enhanced stabilization to multiple membrane proteins compared to the benchmark conventional detergent, DDM. One DTM (i.e., DTM-A6) clearly outperformed DDM in stabilizing human β2 adrenergic receptor (β2AR) and its complex with Gs protein. A further evaluation of this DTM led to a clear visualization of β2AR-Gs complex via electron microscopic analysis. Thus, the current study not only provides novel detergent tools useful for membrane protein study, but also suggests that the dendronic architecture has a role in governing detergent efficacy for membrane protein stabilization.
U2 - 10.1039/c7sc03700g
DO - 10.1039/c7sc03700g
M3 - Journal article
C2 - 29619178
AN - SCOPUS:85034780354
VL - 8
SP - 8315
EP - 8324
JO - Chemical Science
JF - Chemical Science
SN - 2041-6520
IS - 12
ER -
ID: 188453270