Ultrastructural Correlates of Presynaptic Functional Heterogeneity in Hippocampal Synapses

Research output: Contribution to journal › Journal article › Research › peer-review

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Ultrastructural Correlates of Presynaptic Functional Heterogeneity in Hippocampal Synapses. / Maus, Lydia; Lee, ChoongKu; Altas, Bekir; Sertel, Sinem M; Weyand, Kirsten; Rizzoli, Silvio O; Rhee, JeongSeop; Brose, Nils; Imig, Cordelia; Cooper, Benjamin H.

In: Cell Reports, Vol. 30, No. 11, 17.03.2020, p. 3632-3643.e8.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Maus, L, Lee, C, Altas, B, Sertel, SM, Weyand, K, Rizzoli, SO, Rhee, J, Brose, N, Imig, C & Cooper, BH 2020, 'Ultrastructural Correlates of Presynaptic Functional Heterogeneity in Hippocampal Synapses', Cell Reports, vol. 30, no. 11, pp. 3632-3643.e8. https://doi.org/10.1016/j.celrep.2020.02.083

APA

Maus, L., Lee, C., Altas, B., Sertel, S. M., Weyand, K., Rizzoli, S. O., Rhee, J., Brose, N., Imig, C., & Cooper, B. H. (2020). Ultrastructural Correlates of Presynaptic Functional Heterogeneity in Hippocampal Synapses. Cell Reports, 30(11), 3632-3643.e8. https://doi.org/10.1016/j.celrep.2020.02.083

Vancouver

Maus L, Lee C, Altas B, Sertel SM, Weyand K, Rizzoli SO et al. Ultrastructural Correlates of Presynaptic Functional Heterogeneity in Hippocampal Synapses. Cell Reports. 2020 Mar 17;30(11):3632-3643.e8. https://doi.org/10.1016/j.celrep.2020.02.083

Author

Maus, Lydia ; Lee, ChoongKu ; Altas, Bekir ; Sertel, Sinem M ; Weyand, Kirsten ; Rizzoli, Silvio O ; Rhee, JeongSeop ; Brose, Nils ; Imig, Cordelia ; Cooper, Benjamin H. / Ultrastructural Correlates of Presynaptic Functional Heterogeneity in Hippocampal Synapses. In: Cell Reports. 2020 ; Vol. 30, No. 11. pp. 3632-3643.e8.

Bibtex

@article{d06cf00602094066925055c7aeac1bad,

title = "Ultrastructural Correlates of Presynaptic Functional Heterogeneity in Hippocampal Synapses",

abstract = "Although similar in molecular composition, synapses can exhibit strikingly distinct functional transmitter release and plasticity characteristics. To determine whether ultrastructural differences co-define this functional heterogeneity, we combine hippocampal organotypic slice cultures, high-pressure freezing, freeze substitution, and 3D-electron tomography to compare two functionally distinct synapses: hippocampal Schaffer collateral and mossy fiber synapses. We find that mossy fiber synapses, which exhibit a lower release probability and stronger short-term facilitation than Schaffer collateral synapses, harbor lower numbers of docked synaptic vesicles at active zones and a second pool of possibly tethered vesicles in their vicinity. Our data indicate that differences in the ratio of docked versus tethered vesicles at active zones contribute to distinct functional characteristics of synapses.",

author = "Lydia Maus and ChoongKu Lee and Bekir Altas and Sertel, {Sinem M} and Kirsten Weyand and Rizzoli, {Silvio O} and JeongSeop Rhee and Nils Brose and Cordelia Imig and Cooper, {Benjamin H}",

year = "2020",

month = mar,

day = "17",

doi = "10.1016/j.celrep.2020.02.083",

language = "English",

volume = "30",

pages = "3632--3643.e8",

journal = "Cell Reports",

issn = "2211-1247",

publisher = "Cell Press",

number = "11",

}

RIS

TY - JOUR

T1 - Ultrastructural Correlates of Presynaptic Functional Heterogeneity in Hippocampal Synapses

AU - Maus, Lydia

AU - Lee, ChoongKu

AU - Altas, Bekir

AU - Sertel, Sinem M

AU - Weyand, Kirsten

AU - Rizzoli, Silvio O

AU - Rhee, JeongSeop

AU - Brose, Nils

AU - Imig, Cordelia

AU - Cooper, Benjamin H

PY - 2020/3/17

Y1 - 2020/3/17

N2 - Although similar in molecular composition, synapses can exhibit strikingly distinct functional transmitter release and plasticity characteristics. To determine whether ultrastructural differences co-define this functional heterogeneity, we combine hippocampal organotypic slice cultures, high-pressure freezing, freeze substitution, and 3D-electron tomography to compare two functionally distinct synapses: hippocampal Schaffer collateral and mossy fiber synapses. We find that mossy fiber synapses, which exhibit a lower release probability and stronger short-term facilitation than Schaffer collateral synapses, harbor lower numbers of docked synaptic vesicles at active zones and a second pool of possibly tethered vesicles in their vicinity. Our data indicate that differences in the ratio of docked versus tethered vesicles at active zones contribute to distinct functional characteristics of synapses.

AB - Although similar in molecular composition, synapses can exhibit strikingly distinct functional transmitter release and plasticity characteristics. To determine whether ultrastructural differences co-define this functional heterogeneity, we combine hippocampal organotypic slice cultures, high-pressure freezing, freeze substitution, and 3D-electron tomography to compare two functionally distinct synapses: hippocampal Schaffer collateral and mossy fiber synapses. We find that mossy fiber synapses, which exhibit a lower release probability and stronger short-term facilitation than Schaffer collateral synapses, harbor lower numbers of docked synaptic vesicles at active zones and a second pool of possibly tethered vesicles in their vicinity. Our data indicate that differences in the ratio of docked versus tethered vesicles at active zones contribute to distinct functional characteristics of synapses.

U2 - 10.1016/j.celrep.2020.02.083

DO - 10.1016/j.celrep.2020.02.083

M3 - Journal article

C2 - 32187536

VL - 30

SP - 3632-3643.e8

JO - Cell Reports

JF - Cell Reports

SN - 2211-1247

IS - 11

ER -

ID: 237850309

Department of Neuroscience