Activity-dependent volume transmission by transgene NPY attenuates glutamate release and LTP in the subiculum
Research output: Contribution to journal › Journal article › Research › peer-review
Neuropeptide Y (NPY) gene transduction of the brain using viral vectors in epileptogenic regions can effectively suppress seizures in animals, and is being considered as a promising alternative treatment strategy for epilepsy. Therefore, it is fundamental to understand the detailed mechanisms governing the release and action of transgene NPY in neuronal circuitries. Using whole-cell recordings from subicular neurons, we show that in animals transduced by recombinant adeno-associated viral (rAAV) vector carrying the NPY gene, transgene NPY is released during high-frequency activation of CA1-subicular synapses. Released transgene NPY attenuates excitatory synaptic transmission not only in activated, but also in neighboring, non-activated synapses. Such broad action of transgene NPY may prevent recruitment of excitatory synapses in epileptic activity and could play a key role in limiting the spread and generalization of seizures.
Original language | English |
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Journal | Molecular and Cellular Neuroscience |
Volume | 39 |
Issue number | 2 |
Pages (from-to) | 229-37 |
Number of pages | 9 |
ISSN | 1044-7431 |
DOIs | |
Publication status | Published - Oct 2008 |
- Animals, Animals, Newborn, Arginine, Benzazepines, Biophysical Phenomena, Chi-Square Distribution, Electric Stimulation, Excitatory Postsynaptic Potentials, Female, GABA Antagonists, Genetic Vectors, Glutamic Acid, Green Fluorescent Proteins, Hippocampus, In Vitro Techniques, Long-Term Potentiation, Male, Neurons, Neuropeptide Y, Patch-Clamp Techniques, Phosphopyruvate Hydratase, Picrotoxin, Rats, Rats, Sprague-Dawley, Receptors, Neuropeptide Y, Tetrodotoxin, Transgenes, Journal Article, Research Support, Non-U.S. Gov't
Research areas
ID: 186411872