A robust activity marking system for exploring active neuronal ensembles

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

A robust activity marking system for exploring active neuronal ensembles. / Sorensen, Andreas T.; Cooper, Yonatan A.; Baratta, Michael V.; Weng, Feng-Ju; Zhang, Yuxiang; Ramamoorthi, Kartik; Fropf, Robin; LaVerriere, Emily; Xue, Jian; Young, Andrew; Schneider, Colleen; Gotzsche, Casper Rene; Hemberg, Martin; Yin, Jerry C. P.; Maier, Steven F.; Lin, Yingxi.

In: eLife, Vol. 5, No. 9, e13918, 23.09.2016.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Sorensen, AT, Cooper, YA, Baratta, MV, Weng, F-J, Zhang, Y, Ramamoorthi, K, Fropf, R, LaVerriere, E, Xue, J, Young, A, Schneider, C, Gotzsche, CR, Hemberg, M, Yin, JCP, Maier, SF & Lin, Y 2016, 'A robust activity marking system for exploring active neuronal ensembles', eLife, vol. 5, no. 9, e13918. https://doi.org/10.7554/eLife.13918

APA

Sorensen, A. T., Cooper, Y. A., Baratta, M. V., Weng, F-J., Zhang, Y., Ramamoorthi, K., Fropf, R., LaVerriere, E., Xue, J., Young, A., Schneider, C., Gotzsche, C. R., Hemberg, M., Yin, J. C. P., Maier, S. F., & Lin, Y. (2016). A robust activity marking system for exploring active neuronal ensembles. eLife, 5(9), [e13918]. https://doi.org/10.7554/eLife.13918

Vancouver

Sorensen AT, Cooper YA, Baratta MV, Weng F-J, Zhang Y, Ramamoorthi K et al. A robust activity marking system for exploring active neuronal ensembles. eLife. 2016 Sep 23;5(9). e13918. https://doi.org/10.7554/eLife.13918

Author

Sorensen, Andreas T. ; Cooper, Yonatan A. ; Baratta, Michael V. ; Weng, Feng-Ju ; Zhang, Yuxiang ; Ramamoorthi, Kartik ; Fropf, Robin ; LaVerriere, Emily ; Xue, Jian ; Young, Andrew ; Schneider, Colleen ; Gotzsche, Casper Rene ; Hemberg, Martin ; Yin, Jerry C. P. ; Maier, Steven F. ; Lin, Yingxi. / A robust activity marking system for exploring active neuronal ensembles. In: eLife. 2016 ; Vol. 5, No. 9.

Bibtex

@article{713659ca6ae1442fb1efccacef78d036,
title = "A robust activity marking system for exploring active neuronal ensembles",
abstract = "Understanding how the brain captures transient experience and converts it into long lasting changes in neural circuits requires the identification and investigation of the specific ensembles of neurons that are responsible for the encoding of each experience. We have developed a Robust Activity Marking (RAM) system that allows for the identification and interrogation of ensembles of neurons. The RAM system provides unprecedented high sensitivity and selectivity through the use of an optimized synthetic activity-regulated promoter that is strongly induced by neuronal activity and a modified Tet-Off system that achieves improved temporal control. Due to its compact design, RAM can be packaged into a single adeno-associated virus (AAV), providing great versatility and ease of use, including application to mice, rats, flies, and potentially many other species. Cre-dependent RAM, CRAM, allows for the study of active ensembles of a specific cell type and anatomical connectivity, further expanding the RAM system{\textquoteright}s versatility.",
author = "Sorensen, {Andreas T.} and Cooper, {Yonatan A.} and Baratta, {Michael V.} and Feng-Ju Weng and Yuxiang Zhang and Kartik Ramamoorthi and Robin Fropf and Emily LaVerriere and Jian Xue and Andrew Young and Colleen Schneider and Gotzsche, {Casper Rene} and Martin Hemberg and Yin, {Jerry C. P.} and Maier, {Steven F.} and Yingxi Lin",
year = "2016",
month = sep,
day = "23",
doi = "10.7554/eLife.13918",
language = "English",
volume = "5",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications Ltd.",
number = "9",

}

RIS

TY - JOUR

T1 - A robust activity marking system for exploring active neuronal ensembles

AU - Sorensen, Andreas T.

AU - Cooper, Yonatan A.

AU - Baratta, Michael V.

AU - Weng, Feng-Ju

AU - Zhang, Yuxiang

AU - Ramamoorthi, Kartik

AU - Fropf, Robin

AU - LaVerriere, Emily

AU - Xue, Jian

AU - Young, Andrew

AU - Schneider, Colleen

AU - Gotzsche, Casper Rene

AU - Hemberg, Martin

AU - Yin, Jerry C. P.

AU - Maier, Steven F.

AU - Lin, Yingxi

PY - 2016/9/23

Y1 - 2016/9/23

N2 - Understanding how the brain captures transient experience and converts it into long lasting changes in neural circuits requires the identification and investigation of the specific ensembles of neurons that are responsible for the encoding of each experience. We have developed a Robust Activity Marking (RAM) system that allows for the identification and interrogation of ensembles of neurons. The RAM system provides unprecedented high sensitivity and selectivity through the use of an optimized synthetic activity-regulated promoter that is strongly induced by neuronal activity and a modified Tet-Off system that achieves improved temporal control. Due to its compact design, RAM can be packaged into a single adeno-associated virus (AAV), providing great versatility and ease of use, including application to mice, rats, flies, and potentially many other species. Cre-dependent RAM, CRAM, allows for the study of active ensembles of a specific cell type and anatomical connectivity, further expanding the RAM system’s versatility.

AB - Understanding how the brain captures transient experience and converts it into long lasting changes in neural circuits requires the identification and investigation of the specific ensembles of neurons that are responsible for the encoding of each experience. We have developed a Robust Activity Marking (RAM) system that allows for the identification and interrogation of ensembles of neurons. The RAM system provides unprecedented high sensitivity and selectivity through the use of an optimized synthetic activity-regulated promoter that is strongly induced by neuronal activity and a modified Tet-Off system that achieves improved temporal control. Due to its compact design, RAM can be packaged into a single adeno-associated virus (AAV), providing great versatility and ease of use, including application to mice, rats, flies, and potentially many other species. Cre-dependent RAM, CRAM, allows for the study of active ensembles of a specific cell type and anatomical connectivity, further expanding the RAM system’s versatility.

U2 - 10.7554/eLife.13918

DO - 10.7554/eLife.13918

M3 - Journal article

C2 - 27661450

VL - 5

JO - eLife

JF - eLife

SN - 2050-084X

IS - 9

M1 - e13918

ER -

ID: 167915356