Schwann cells transduced with a lentiviral vector encoding Fgf-2 promote motor neuron regeneration following sciatic nerve injury
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Schwann cells transduced with a lentiviral vector encoding Fgf-2 promote motor neuron regeneration following sciatic nerve injury. / Allodi, Ilary; Mecollari, Vasil; González-Pérez, Francisco; Eggers, Ruben; Hoyng, Stefan; Verhaagen, Joost; Navarro, Xavier; Udina, Esther.
In: Glia, Vol. 62, No. 10, 2014, p. 1736-1746.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Schwann cells transduced with a lentiviral vector encoding Fgf-2 promote motor neuron regeneration following sciatic nerve injury
AU - Allodi, Ilary
AU - Mecollari, Vasil
AU - González-Pérez, Francisco
AU - Eggers, Ruben
AU - Hoyng, Stefan
AU - Verhaagen, Joost
AU - Navarro, Xavier
AU - Udina, Esther
N1 - © 2014 Wiley Periodicals, Inc.
PY - 2014
Y1 - 2014
N2 - Fibroblast growth factor 2 (FGF-2) is a trophic factor expressed by glial cells and different neuronal populations. Addition of FGF-2 to spinal cord and dorsal root ganglia (DRG) explants demonstrated that FGF-2 specifically increases motor neuron axonal growth. To further explore the potential capability of FGF-2 to promote axon regeneration, we produced a lentiviral vector (LV) to overexpress FGF-2 (LV-FGF2) in the injured rat peripheral nerve. Cultured Schwann cells transduced with FGF-2 and added to collagen matrix embedding spinal cord or DRG explants significantly increased motor but not sensory neurite outgrowth. LV-FGF2 was as effective as direct addition of the trophic factor to promote motor axon growth in vitro. Direct injection of LV-FGF2 into the rat sciatic nerve resulted in increased expression of FGF-2, which was localized in the basal lamina of Schwann cells. To investigate the in vivo effect of FGF-2 overexpression on axonal regeneration after nerve injury, Schwann cells transduced with LV-FGF2 were grafted in a silicone tube used to repair the resected rat sciatic nerve. Electrophysiological tests conducted for up to 2 months after injury revealed accelerated and more marked reinnervation of hindlimb muscles in the animals treated with LV-FGF2, with an increase in the number of motor and sensory neurons that reached the distal tibial nerve at the end of follow-up.
AB - Fibroblast growth factor 2 (FGF-2) is a trophic factor expressed by glial cells and different neuronal populations. Addition of FGF-2 to spinal cord and dorsal root ganglia (DRG) explants demonstrated that FGF-2 specifically increases motor neuron axonal growth. To further explore the potential capability of FGF-2 to promote axon regeneration, we produced a lentiviral vector (LV) to overexpress FGF-2 (LV-FGF2) in the injured rat peripheral nerve. Cultured Schwann cells transduced with FGF-2 and added to collagen matrix embedding spinal cord or DRG explants significantly increased motor but not sensory neurite outgrowth. LV-FGF2 was as effective as direct addition of the trophic factor to promote motor axon growth in vitro. Direct injection of LV-FGF2 into the rat sciatic nerve resulted in increased expression of FGF-2, which was localized in the basal lamina of Schwann cells. To investigate the in vivo effect of FGF-2 overexpression on axonal regeneration after nerve injury, Schwann cells transduced with LV-FGF2 were grafted in a silicone tube used to repair the resected rat sciatic nerve. Electrophysiological tests conducted for up to 2 months after injury revealed accelerated and more marked reinnervation of hindlimb muscles in the animals treated with LV-FGF2, with an increase in the number of motor and sensory neurons that reached the distal tibial nerve at the end of follow-up.
KW - Animals
KW - Axons/physiology
KW - Cell Proliferation/physiology
KW - Cells, Cultured
KW - Coculture Techniques
KW - Female
KW - Fibroblast Growth Factor 2/genetics
KW - Ganglia, Spinal/physiopathology
KW - Genetic Vectors
KW - HEK293 Cells
KW - Hindlimb/physiopathology
KW - Humans
KW - Lentivirus/genetics
KW - Motor Neurons/physiology
KW - Muscle, Skeletal/physiopathology
KW - Nerve Regeneration
KW - Rats, Inbred F344
KW - Schwann Cells/metabolism
KW - Sciatic Nerve/injuries
KW - Sensory Receptor Cells/physiology
KW - Spinal Cord/physiopathology
KW - Tibial Nerve/physiopathology
KW - Tissue Scaffolds
U2 - 10.1002/glia.22712
DO - 10.1002/glia.22712
M3 - Journal article
C2 - 24989458
VL - 62
SP - 1736
EP - 1746
JO - GLIA
JF - GLIA
SN - 0894-1491
IS - 10
ER -
ID: 227433606