Neuroprotection and axonal regeneration after lumbar ventral root avulsion by re-implantation and mesenchymal stem cells transplant combined therapy
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Neuroprotection and axonal regeneration after lumbar ventral root avulsion by re-implantation and mesenchymal stem cells transplant combined therapy. / Torres-Espín, Abel; Corona-Quintanilla, Dora Luz; Forés, Joaquim; Allodi, Ilary; González, Francisco; Udina, Esther; Navarro, Xavier.
In: Neurotherapeutics, Vol. 10, No. 2, 2013, p. 354-368.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Neuroprotection and axonal regeneration after lumbar ventral root avulsion by re-implantation and mesenchymal stem cells transplant combined therapy
AU - Torres-Espín, Abel
AU - Corona-Quintanilla, Dora Luz
AU - Forés, Joaquim
AU - Allodi, Ilary
AU - González, Francisco
AU - Udina, Esther
AU - Navarro, Xavier
PY - 2013
Y1 - 2013
N2 - Ventral spinal root avulsion causes complete denervation of muscles in the limb and also progressive death of segmental motoneurons (MN) leading to permanent paralysis. The chances for functional recovery after ventral root avulsion are very poor owing to the loss of avulsed neurons and the long distance that surviving neurons have to re-grow axons from the spinal cord to the corresponding targets. Following unilateral avulsion of L4, L5 and L6 spinal roots in adult rats, we performed an intraspinal transplant of mesenchymal stem cells (MSC) and surgical re-implantation of the avulsed roots. Four weeks after avulsion the survival of MN in the MSC-treated animals was significantly higher than in vehicle-injected rats (45% vs. 28%). Re-implantation of the avulsed roots in the injured spinal cord allowed the regeneration of motor axons. By combining root re-implantation and MSC transplant the number of surviving MN at 28 days post-injury was higher (60%) than in re-implantation alone animals (46%). Electromyographic tests showed evidence of functional re-innervation of anterior tibialis and gastrocnemius muscles by the regenerated motor axons only in rats with the combined treatment. These results indicate that MSC are helpful in enhancing neuronal survival and increased the regenerative growth of injured axons. Surgical re-implantation and MSC grafting combined had a synergic neuroprotective effect on MN and on axonal regeneration and muscle re-innervation after spinal root avulsion.
AB - Ventral spinal root avulsion causes complete denervation of muscles in the limb and also progressive death of segmental motoneurons (MN) leading to permanent paralysis. The chances for functional recovery after ventral root avulsion are very poor owing to the loss of avulsed neurons and the long distance that surviving neurons have to re-grow axons from the spinal cord to the corresponding targets. Following unilateral avulsion of L4, L5 and L6 spinal roots in adult rats, we performed an intraspinal transplant of mesenchymal stem cells (MSC) and surgical re-implantation of the avulsed roots. Four weeks after avulsion the survival of MN in the MSC-treated animals was significantly higher than in vehicle-injected rats (45% vs. 28%). Re-implantation of the avulsed roots in the injured spinal cord allowed the regeneration of motor axons. By combining root re-implantation and MSC transplant the number of surviving MN at 28 days post-injury was higher (60%) than in re-implantation alone animals (46%). Electromyographic tests showed evidence of functional re-innervation of anterior tibialis and gastrocnemius muscles by the regenerated motor axons only in rats with the combined treatment. These results indicate that MSC are helpful in enhancing neuronal survival and increased the regenerative growth of injured axons. Surgical re-implantation and MSC grafting combined had a synergic neuroprotective effect on MN and on axonal regeneration and muscle re-innervation after spinal root avulsion.
KW - Animals
KW - Axons/physiology
KW - Behavior, Animal/physiology
KW - Cell Count
KW - Cell Survival/physiology
KW - Cells, Cultured
KW - Female
KW - Fibroblasts/physiology
KW - Image Processing, Computer-Assisted
KW - Immunohistochemistry
KW - Mesenchymal Stem Cell Transplantation/methods
KW - Motor Activity/physiology
KW - Motor Neurons/physiology
KW - Nerve Regeneration/physiology
KW - Nervous System Diseases/prevention & control
KW - Neurites/physiology
KW - Organ Culture Techniques
KW - Rats
KW - Rats, Sprague-Dawley
KW - Spinal Nerve Roots
U2 - 10.1007/s13311-013-0178-5
DO - 10.1007/s13311-013-0178-5
M3 - Journal article
C2 - 23440700
VL - 10
SP - 354
EP - 368
JO - Neurotherapeutics
JF - Neurotherapeutics
SN - 1933-7213
IS - 2
ER -
ID: 227433810