In vitro comparison of motor and sensory neuron outgrowth in a 3D collagen matrix
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In vitro comparison of motor and sensory neuron outgrowth in a 3D collagen matrix. / Allodi, Ilary; Guzmán-Lenis, Mónica-Sofía; Hernàndez, Joaquim; Navarro, Xavier; Udina, Esther.
In: Journal of Neuroscience Methods, Vol. 198, No. 1, 2011, p. 53-61.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - In vitro comparison of motor and sensory neuron outgrowth in a 3D collagen matrix
AU - Allodi, Ilary
AU - Guzmán-Lenis, Mónica-Sofía
AU - Hernàndez, Joaquim
AU - Navarro, Xavier
AU - Udina, Esther
N1 - Copyright © 2011 Elsevier B.V. All rights reserved.
PY - 2011
Y1 - 2011
N2 - In this work we set up an in vitro model, based on organotypic cultures of spinal cord slices and dorsal root ganglia explants from P7 rats, embedded in a collagen matrix and cultured under the same conditions. As specific reinnervation of end-organs is still an unresolved issue in peripheral nerve research, we characterized a model that allows us to compare under the same conditions motor and sensory neuron regeneration. RT97 labeling was used to visualize the regenerating neurites that extended in the collagen gel from both motor neurons in the spinal cord slices and sensory neurons in the DRG explants after a few days in vitro. By adding different neurotrophic factors in the collagen matrix, we evaluated the reliability of DRG and spinal cord preparations. Moreover, we also set up a co-culture with dissociated Schwann cells to further mimic the permissive environment of the peripheral nerve. Thus, these in vitro models can be useful tools to investigate mechanisms for the selective regeneration of sensory and motor neurons, which can be translated into in vivo models.
AB - In this work we set up an in vitro model, based on organotypic cultures of spinal cord slices and dorsal root ganglia explants from P7 rats, embedded in a collagen matrix and cultured under the same conditions. As specific reinnervation of end-organs is still an unresolved issue in peripheral nerve research, we characterized a model that allows us to compare under the same conditions motor and sensory neuron regeneration. RT97 labeling was used to visualize the regenerating neurites that extended in the collagen gel from both motor neurons in the spinal cord slices and sensory neurons in the DRG explants after a few days in vitro. By adding different neurotrophic factors in the collagen matrix, we evaluated the reliability of DRG and spinal cord preparations. Moreover, we also set up a co-culture with dissociated Schwann cells to further mimic the permissive environment of the peripheral nerve. Thus, these in vitro models can be useful tools to investigate mechanisms for the selective regeneration of sensory and motor neurons, which can be translated into in vivo models.
KW - Animals
KW - Animals, Newborn
KW - Cells, Cultured
KW - Coculture Techniques/methods
KW - Collagen/metabolism
KW - Extracellular Matrix/physiology
KW - Ganglia, Spinal/cytology
KW - Humans
KW - In Situ Nick-End Labeling/methods
KW - Motor Neurons/cytology
KW - Nerve Growth Factors/pharmacology
KW - Neurites/drug effects
KW - Organ Culture Techniques
KW - Rats
KW - Rats, Sprague-Dawley
KW - Schwann Cells/physiology
KW - Sensory Receptor Cells/cytology
KW - Spinal Cord/cytology
KW - Time Factors
U2 - 10.1016/j.jneumeth.2011.03.006
DO - 10.1016/j.jneumeth.2011.03.006
M3 - Journal article
C2 - 21402104
VL - 198
SP - 53
EP - 61
JO - Journal of Neuroscience Methods
JF - Journal of Neuroscience Methods
SN - 0165-0270
IS - 1
ER -
ID: 227434007