Rasmussen Lab

The purpose of the research in my lab is to understand how G-protein coupled receptors (GPCRs) and neurotransmitter transporters function at the molecular level.

Søren Rasmussen

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Personal info

Name                Søren Gøgsig Faarup Rasmussen
                          Born November 24th 1969, Denmark
Contact             Department of Neuroscience, University of Copenhagen, 
                          Panum 07.5.77 (Mærsk bldg.), Blegdamsvej 3, DK-2200, Copenhagen N
                          E-mail:
sgfr@sund.ku.dk, Phone: (+45) 2339-7812

Education

1990 - 1994            B.Sc. Biology, Faculty of Science, University of Copenhagen
1994 - 1998            M.Sc. Human Biology, Faculty of Health and Medical Sciences, University of Copenhagen
1999 - 2002            Ph.D. Faculty of Health and Medical Sciences, University of Copenhagen

Positions

1996 - 1998            The National University Hospital of Copenhagen, Lab. for Molecular Pharmacology RH-6321, Master thesis
1998 - 2002            University of Copenhagen, Dept. of Medical Physiology, Ph.D. thesis
2002 - 2005            University of Copenhagen, Dept. of Pharmacology, Post doc
2005 - 2011            Stanford University, Dept. of Molecular & Cellular Physiology, Post doc
2011 - 2017            University of Copenhagen, Dept. of Neuroscience & Pharmacology
2011 -                      Assistant professor
2015 -                      Associate professor
2017 -                     University of Copenhagen, Department of Neuroscience

Publications

Articles published in Nature (8 original, 1 review), Science (2), Nature Struct. & Mol. Biol. (1), Nature Methods (3), Nature Protocols (1), Nature Communications (2), Science Advances (1), PNAS (4), EMBO J. (1), Elife (1), J. Biol. Chem. (4). Three review articles and five book chapters. Co-inventor on four patents.

Google Scholar: ~16,500 citations; H-index = 38

Awards

Annual Biotechnology Award for Young Researchers 2008 from the Danish Biotechnological Society

Lundbeckfonden: Junior Group Leader Fellowship 2009

 

 

 

 

 

 

 

 

 

 

Peer review

42  Wiuf A. Steffen J., Bécares E.R., Grønberg C., Mahato R., Rasmussen S.G.F., Andersson M., Croll T., Gourdon P., Gotfryd K., The two-domain elevator-type mechanism of zinc-transporting ZIP proteins. Science Advances (2022) 8: eabn4331, DOI: 10.1126/sciadv.abn4331

41 Nielsen A.K., Möller I.R., Wang Y., Rasmussen S.G.F. , Lindorff-Larsen K., Rand K.D., Loland C.J., Substrate-induced conformational dynamics of the dopamine transporter. Nature Communications (2019) 10:2714. DOI: 10.1038/s41467-019-10449-w

40 Du Y.§, Duc N.M.§, Rasmussen S.G.F. §, Hilger D., Kubiak. X., Wang L., Bohon J., Kim H.R., Wegrecki M., Asuru A., Jeong K.M., Lee J., Chance. M.R., Lodowski D.T., Kobilka B.K., Chung K.Y., Assembly of a GPCR-G protein complex. Cell (2019) 177:1232-1242.
§Authors contributed equally to this work

39 Möller I.R., Slivacka M., Nielsen A.K., Rasmussen S.G.F. , Gether U., Loland C.J., Rand K.D., Conformational Dynamics of the Human Serotonin Transporter during Substrate and Drug Binding. Nature Communications (2019) 10:1687. DOI:10.1038/s41467-019-09675-z

38 Walsh S.M., Mathiasen S., Christensen S.M., Fay J.F., King C., Provasi D., Borrero E., Rasmussen S.G.F. , Fung J.J., Filizola M., Hristova K.,Kobilka B. Farrens D.L., Stamou D., Single proteoliposome high content analysis reveals differences in the homo-oligomerization of class A GPCRs. Biophys. J. (2018) 115:300-312

37 McMahon C., Baier A.S., Pascolutti R., Wegrecki M., Zheng S., Ong J.X., Erlandson S.C., Hilger D., Rasmussen S.G.F. , Ring A.M., Manglik A., Kruse A.C., Yeast surface display platform for rapid discovery of conformationally selective nanobodies. Nature Structural and Molecular Biology (2018) 25:289-296

36 Boyhus L.E., Danielsen M., Bengtson N.S., Kunze M.B.A., Kubiak X., Sminia T.J., Løper J.H., Tran P.T., Lindorff-Larsen K., Rasmussen S.G.F. , Mathiesen J.M., Pedersen D.S., Gs protein peptidomimetics as allosteric modulators of the β2-adrenergic receptor, RSC Advances (2018) 8:2219-2238

35 Clark L., Dikiy I., Chapman K., Rödström K.E.J., Aramini J., LeVine M.V., Khelashvili G., Rasmussen S.G.F. , Gardner K.H., Rosenbaum D.M., Ligand modulation of sidechain dynamics in a wild-type human GPCR, Elife (2017), DOI: 10.7554/eLife.28505

34 DeVree B.T., Mahoney J.P., Vélez-Ruiz G.A., Rasmussen S.G.F. , Kuszak A.J., Edwald E., Fung J.J., Manglik A., Masureel M., Du Y., Matt R.A., Pardon E., Steyaert J., Kobilka B.K. and Sunahara R.K., Allosteric coupling from G protein to the agonist binding pocket in GPCRs. Nature (2016) 535:182-6

33 Mathiesen S., Christensen S.M., Fung J.J., Rasmussen S.G.F. , Fay J.F., Jørgensen S.K., Farrens D.L., Byrne M., Kobilka B., Stamou D., High-content analysis of single proteoliposome composition used to assay quantitatively GPCR oligomerization. Nature Methods (2014) 11:931-4

32 Pardon E., Laeremans T., Triest S., Rasmussen S.G.F. , Wohlkonig A., Ruf A., Muyldermans S., Hol W., Kobilks B.K., Steyaert J. A General Protocol for Generation of Nanobodies for Structural Biology. Nature Protocols (2014) 9:674-693

31 Staus D., Wingler L.M., Strachan R.T., Rasmussen S.G.F. , Pardon E., Ahn S., Steyaert J., Kobilka B., Lefkowitz R.J., Regulation of β2‐Adrenergic Receptor‐2 Function by Conformationally Selective Single‐domain Intrabodies. Mol. Pharmacol. (2014) 85:472-481

30 Chae P.S., Kruse A.C., Gotfryd K., Rana R.R., Rasmussen S.G.F. , Cho K.H., Bae E., Guan L., Loland C.J., Kobilka B., Byrne B., Gellman S.H. Novel tripod amphiphiles for membrane protein analysis. Chem. Eur. J. (2013) 19:15645-15651

29 Irannejad R., Tomshine J.C., Tomshine J.R., Chevalier M., Mahoney J.P., Steyaert J., Rasmussen S.G.F. , Sunahara R.K., El-Samad H., Huang B., von Zastrow M. Conformational biosensors reveal GPCR signaling from endosomes, Nature (2013) 495:534-538

28 Chae P.S., Rana R.R., Gotfryd K., Rasmussen S.G.F. , Kruse A.C., Cho K.H., Capaldi S., Kobilka B., Loland C.J., Gether U., Banerjee S., Byrne B., Lee J., Gellman S.H. Glucose-Neopentyl Glycol (GNG) amphiphiles for Membrane Protein Solubilization, Stabilization and Crystallization. ChemCommun (2013) 49:2287-89

27 Zocher M., Zhang C., Rasmussen S.G.F. , Kobilka B.K., Müller D.J., Cholesterol increases the kinetic, energetic, and mechanical stability of the human β2 adrenergic receptor. Proc. Natl. Acad. Sci. USA. (2012) 109:3463-72

26 Chae P.S., Rasmussen S.G.F. , Rana R.R., Gotfryd K., Kruse A.C., Nurva S., Gether U.,Guan L., Loland C.J., Byrne B., Kobilka B., Gellman S.H. A New Class of Amphiphiles Bearing Rigid Hydrophobic Groups for Solubilization and Stabilization of Membrane Proteins. Chem. Eur. J. (2012)

25 Chung K.Y§, Rasmussen S.G.F. §, Liu T. §, Li S., DeVree B.T., Chae P.S., Calinski D., Kobilka B.K., Woods V.L., Sunahara R.K. β2 Adrenergic Receptor-Induced conformational changes in the heterotrimeric G protein. Nature (2011) 477: 611-615.
§Authors contributed equally to this work

24 Westfield G.§, Rasmussen S.G.F. §, Su M. §, Dutta S. §, DeVree B.T., Chung K.Y.,Calinski D., Velez-Ruiz G., Oleskie A., Pardon E., Chae P.S., Liu T., Li. S., Woods V.L., Steyeart J., Kobilka B.K., Sunahara R.K., Skiniotis G., Structural Rearrangements of the Gαs α-Helical Domain in the β2-adrenoceptor Gs Complex. Proc. Natl. Acad. Sci. USA. (2011) 108:16086-91.
§Authors contributed equally to this work

23 Rasmussen S.G.F. §, DeVree B.T. §, Zho Y., Kruse A.C., Chung K.Y., Kobilka T.S., Thian F.S., Chae P.S., Pardon E., Calinski D., Mathiesen J.M., Shah S.T.A., Lyons J.A., Caffrey M., Gellman S.H., Steyaert J., Skiniotis G., Weis W.I., Sunahara R.K., Kobilka B.K., Crystal Structure of the β2 Adrenergic Receptor-Gs Protein Complex. Nature (2011) 477:550-555.
§Authors contributed equally to this work

22 Selao T.T., Branca R. , Chae P.S., Lehtiö J., Gellman S.H., Rasmussen S.G.F. , Nordlund S., Norén A., Identification of the chromophore membrane protein complex formed under different nitrogen availability conditions in Rhodospirilium rubrum. J. Prot. Res. (2011) 10:2703-2714

21 Rosenbaum D.M., Zhang C., Lyons J., Holl R., Aragao D., Arlow D.H., Rasmussen S.G.F. , Choi H.J., DeVree B.T., Sunahara R.K., Chae P.S., Gellman S.H., Dror R.O., Shaw D.E., Weis W.I., Caffrey M., Gmeiner P., Kobilka B.K., Structure and Function of an Irreversible Agonist- β2 Adrenoceptor Complex, Nature (2011) 469: 236-240

20 Rasmussen S.G.F. §, Choi H.J. §, Fung J.J.§, Casarosa P., Pardon E., Chae P.S., DeVree B.T., Rosenbaum D.M., Kobilka T.S., Thian F.S., Schnapp A., Konetzki I., Sunahara R.K., Gellman S.H., Pautsch A., Steyaert J., Weis W.I., Kobilka B.K., Structure of an Nanobody-Stabilized Active State of the β2 Adrenoceptor, Nature (2011) 469:175-180.
§Authors contributed equally to this work

19 Chae P.S., Gotfryd K., Pecyna J., Miercke L.J.W., Rasmussen S.G.F. , Robins R.A., Loland C.J., Kobilka B., Stroud R., Byrne B., Gether U., Gellman S.H. Tandem Facial Amphiphiles for Membrane Protein Stabilization. J. Am. Chem. Soc. (2010) 132:16750-16752

18 Chae P.S., Rasmussen S.G.F. , Rana R., Gotfryd K., Chandra R., Goren M.A., Kruse A.C., Nurva S., Loland C.J., Pierre Y., Drew D., Popot J.L. Picot D., Fox B.G., Guan L., Gether U.,Byrne B., Kobilka B., Gellman S.H., Maltose-neopentyl glycol (MNG) amphiphiles for solubilization, stabilization and crystallization of membrane proteins. Nature Methods (2010) 7:1003-8

17 Bokoch M.P., Zou Y., Rasmussen S.G.F. , Liu C.W., Nygaard R., Rosenbaum D.M., Fung J.J., Choi H.J., Thian F.S., Kobilka T.S., Puglisi J.D., Weis W.I., Pardo L., Prossor R.S., Mueller L., and Kobilka B.K., Ligand-specific regulation of the extracellular surface of a G protein coupled receptor. Nature (2010) 463:108-112

16 Eriksen J.§, Rasmussen S.G.F. §, Rasmussen T.N., Vaegter C.B., Cha J.H., Zhu M.F., Fog J.U., J.H., Newman A.H., Gether U., Visualization of dopamine transporter trafficking in live neurons by use of fluorescent cocaine analogues. J. Neuroscience (2009) 29:6794-808.
§Authors contributed equally to this work

15 Yao Y.J., Whorton M.R., Vélez-Ruiz G., Rasmussen S.G.F. , DeVree B.T., Deupi X., Sunahara R.K., and Kobilka B.K., The effect of ligand efficacy on the formation and stability of a GPCR-G protein complex. Proc. Natl. Acad. Sci. USA. (2009) 106:9501-9506

14 Richter W., Day P.W., Agrawal R., Bruss M., Granier S., Wang Y., Rasmussen S.G.F. , Horner K., Wang P., Lei T., Patterson A.J., Kobilka B., and Conti M., Signaling from β1- and β2-adrenergic receptors is defined by differential interactions with PDE4. EMBO J. (2008)27:384-393

13 Cherezov V., Rosenbaum D.M., Hanson M.A., Rasmussen S.G.F. , Thian F.S, Kobilka T.S, Choi H.J., Kuhn P., Weis W.I., Kobilka B.K, Stevens R.C, High Resolution Crystal Structure of a Modified Human β2-Adrenergic G protein-Coupled Receptor, Science (2007) 318:1258-1265

12 Rosenbaum D.M., Cherezov V., Hanson M.A., Rasmussen S.G.F. , Thian F.S., Kobilka T.S., Choi H.J., Yao Y.J., Kuhn P., Weis W.I., Stevens R.C., Kobilka B.K, GPCR Engineering Yields High-resolution Structural Insights into β2 Adrenergic Receptor Function, Science (2007) 318:1266-1273

11 Rasmussen S.G.F. §, Choi H.J. §, Rosenbaum D.M. §, Kobilka T.S., Thian F.S., Edwards P., Burghammer M., Ratnala V.R.P., Sanishvili R., Fischetti R., Schertler G.F.X, Weis W.I., Kobilka B.K., Crystal structure of the human β2 adrenergic G-protein-coupled receptor, Nature (2007) 450:383-387.
§Authors contributed equally to this work

10 Day P.W.§, Rasmussen S.G.F. §, Parnot C., Fung J.J., Masood A., Kobilka T.S., Yao Y.J., Choi H.J., Weis W.I., Rohrer D.K., Kobilka B.K., A monoclonal antibody for G protein coupled receptor crystallography, Nature Methods (2007) 4:927-929.
§Authors contributed equally to this work.

9 Plenge P., Gether U., and Rasmussen S.G.F. , Allosteric effects of R- and S-citalopram on the human 5-HT transporter: Evidence for distinct high- and low-affinity binding sites. Eur. J. Pharmacol. (2007) 567:1-9

8 Whorton M.R., Bokoch M.P., Rasmussen S.G.F. , Huang B., Zare R.N., Kobilka B., and Sunahara R.K. A monomeric G protein coupled receptor isolated in a high-density lipoprotein particle efficiently activates its G protein. Proc. Natl. Acad. Sci. USA. (2007) 104:7682-7687

7 Cha J.H., Zhu M.F., Adkins E.M., Rasmussen S.G.F. , Loland C.J., Schonenberger B., Gether U., Newman A.H., Rhodamine-Labeled 2b-Carbomethoxy-3b-(3,4-dichlorophenyl)tropane Analogues as High-Affinity Fluorescent Probes for the Dopamine Transporter, J. Med. Chem. (2005) 48:7513-7516

6 Rasmussen S.G.F. § and Gether U.§, Purification and Fluorescent Labeling of the Human Serotonin Transporter, Biochemistry (2005) 44: 3494-3505
§Authors share correspondence.

5 Ballesteros J.A. §, Jensen A.D.§, Liapakis G. §, Rasmussen S.G.F. §, Shi L., Gether U., Javitch J.A., Activation of the β2 Adrenergic Receptor Involves Disruption of an Ionic Lock Between the Cytoplasmic Ends of Transmembrane Segments 3 and 6, J. Biol. Chem. (2001) 276:29171-29177.
§Authors contributed equally to this work.

4 Jensen A.D., Guarnieri F., Rasmussen S.G.F. , Asmar F., Ballesteros, J.A., Gether U, Agonist-Induced Conformational Changes at the Cytoplasmic Side of TM6 in the β2 Adrenergic Receptor Mapped by Site-Selective Fluorescent Labeling, J. Biol. Chem. (2001) 276: 9279-9290.

3 Rasmussen S.G.F. , Carroll F.I., Maresch M., Jensen A.D., Tate C.G., Gether U.,Biophysical Characterization of the Cocaine Binding Pocket in the Serotonin Transporter Using a Fluorescent Cocaine Analogue as a Molecular Reporter, J. Biol. Chem. (2001) 276: 4717-4723.

2 Ghanouni P., Schambye H., Seifert R., Lee T.W., Rasmussen S.G.F. , Gether U., Kobilka B. K., The Effect of pH on β2 Adrenoceptor Function: Evidence for protonation-dependent activation, J. Biol. Chem. (2000) 275: 3121-3127.

1 Rasmussen S.G.F. , Jensen A.D., Liapakis G., Ghanouni P., Javitch J.A., Gether U., Mutation of a Highly Conserved Aspartic Acid in the β2 Adrenergic Receptor: Constitutive Activation, Structural Instability and Conformational Rearrangement of Transmembrane Segment 6, Mol. Pharmacol. (1999), 56: 175-184

Reviews

3 Rosenbaum D.M. Rasmussen S.G.F. , and Kobilka B.K, Structural and mechanistic insights into G-protein coupled receptor function, Nature (2009) 459:356-363

2 Rasmussen S.G.F. , Adkins E.M., Carroll F.I., Maresch M., Gether U. Structural and Functional Probing of the Biogenic Amine Transporters by Fluorescence Spectroscopy, Eur. J. Pharmacol., (2003) 479: 13-22.

1 Gether U., Asmar F., Meinild A.K., Rasmussen S.G.F. Structural basis for G-protein coupled receptor activation, Pharmacology and Toxicology (2002) 91: 304-312.

Chapters

5 Kuszak A.J., Yao X.J., Rasmussen S.G.F. , Kobilka B.K., and Sunahara R.K., Functional studies of isolated GPCR-G protein complexes in the membrane bilayer of lipoprotein particles, In G Protein-Coupled Receptors (eds. S. Siehler and and G. Milligan), ISBN-978-0-521-11208-6, Cambridge University Press, pp.32-52 (2010)

4 Weis W.I., Rosenbaum D.M., Rasmussen S.G.F. , et. al. Crystal Structures of the β2-Adrenergic Receptor, In From Molecules to Medicines, Structure of Biological Macromolecules and Its Relevance in Combating New Diseases and Bioterrorism(eds. J.L. Sussman and P. Spadon) ISBN: 978-90-481-2338-4, Springer Netherlands, pp. 217-230 (2009)

3 Rosenbaum D.M. Rasmussen S.G.F. , and Kobilka B.K, The β2 Adrenergic Receptor as a Model for G Protein-Coupled Receptor Structure and Activation by Diffusible Hormones, In The Handbook of Cell Signaling (eds. R. Bradshaw and E. Dennis), ISBN-10: 0-12-374145-9, Elsevier Inc., (2009)

2 Rasmussen S.G.F. and Gether U., Molecular mechanisms of GPCR activation, In G Protein-Coupled Receptors as Drug Targets: Analysis of Activation and Constitutive Activity, Volume 24 (eds. R. Seifert and T. Wieland), ISBN: 3-527-30819-9, WILEY-VCH Verlag GmbH, Weinheim, pp. 27-42 (2006)

1 Rasmussen S.G.F. and Gether U., Structural mechanics of GPCR activation, In Understanding G-protein coupled receptors and their role in the CNS (eds. M.N. Pangalos and C.H. Davies), ISBN: 0-19-850916-2, Oxford University Press, Oxford: New York, pp. 43-62 (2002)

 

 

 

 

Lab members

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Søren Gøgsig Faarup Rasmussen Associate Professor Rasmussen lab Billede af Søren Gøgsig Faarup Rasmussen