Rasmussen Lab

• Du Y.§, Duc N.M.§, Rasmussen S.G.F. §, Hilger D., Kubiak. X., Wang L., Bohon J., Kim H.R., Wegrecki M., Asuru A., Jeong K.M., Lee J., Chance. M.R., Lodowski D.T., Kobilka B.K., Chung K.Y., Assembly of a GPCR-G protein complex. Cell (2019) 177:1232-1242. §Authors contributed equally to this work
• McMahon C., Baier A.S., Pascolutti R., Wegrecki M., Zheng S., Ong J.X., Erlandson S.C., Hilger D., Rasmussen S.G.F. , Ring A.M., Manglik A., Kruse A.C., Yeast surface display platform for rapid discovery of conformationally selective nanobodies. Nature Structural and Molecular Biology (2018) 25:289-296
• Clark L., Dikiy I., Chapman K., Rödström K.E.J., Aramini J., LeVine M.V., Khelashvili G., Rasmussen S.G.F. , Gardner K.H., Rosenbaum D.M., Ligand modulation of sidechain dynamics in a wild-type human GPCR. Elife (2017), DOI: 10.7554/eLife.28505
• DeVree B.T., Mahoney J.P., Vélez-Ruiz G.A., Rasmussen S.G.F. , Kuszak A.J., Edwald E., Fung J.J., Manglik A., Masureel M., Du Y., Matt R.A., Pardon E., Steyaert J., Kobilka B.K. and Sunahara R.K., Allosteric coupling from G protein to the agonist binding pocket in GPCRs. Nature (2016) 535:182-6
• Irannejad R., Tomshine J.C., Tomshine J.R., Chevalier M., Mahoney J.P., Steyaert J., Rasmussen S.G.F. , Sunahara R.K., El-Samad H., Huang B., von Zastrow M. Conformational biosensors reveal GPCR signaling from endosomes. Nature (2013) 495:534-538
• Rasmussen S.G.F. §, DeVree B.T. §, Zho Y., Kruse A.C., Chung K.Y., Kobilka T.S., Thian F.S., Chae P.S., Pardon E., Calinski D., Mathiesen J.M., Shah S.T.A., Lyons J.A., Caffrey M., Gellman S.H., Steyaert J., Skiniotis G., Weis W.I., Sunahara R.K., Kobilka B.K., Crystal Structure of the β2 Adrenergic Receptor-Gs Protein Complex. Nature (2011) 477:550-555. §Authors contributed equally to this work
• Chung K.Y§, Rasmussen S.G.F. §, Liu T. §, Li S., DeVree B.T., Chae P.S., Calinski D., Kobilka B.K., Woods V.L., Sunahara R.K. β2 Adrenergic Receptor-Induced conformational changes in the heterotrimeric G protein. Nature (2011) 477: 611-615. §Authors contributed equally to this work
• Rasmussen S.G.F. §, Choi H.J. §, Fung J.J.§, Casarosa P., Pardon E., Chae P.S., DeVree B.T., Rosenbaum D.M., Kobilka T.S., Thian F.S., Schnapp A., Konetzki I., Sunahara R.K., Gellman S.H., Pautsch A., Steyaert J., Weis W.I., Kobilka B.K., Structure of an Nanobody-Stabilized Active State of the β2 Adrenoceptor. Nature (2011) 469:175-180. §Authors contributed equally to this work
• Westfield G.§, Rasmussen S.G.F. §, Su M. §, Dutta S. §, DeVree B.T., Chung K.Y.,Calinski D., Velez-Ruiz G., Oleskie A., Pardon E., Chae P.S., Liu T., Li. S., Woods V.L., Steyeart J., Kobilka B.K., Sunahara R.K., Skiniotis G., Structural Rearrangements of the Gαs α-Helical Domain in the β2-adrenoceptor Gs Complex. Proc. Natl. Acad. Sci. USA. (2011) 108:16086-91. §Authors contributed equally to this work
• Chae P.S., Rasmussen S.G.F. , Rana R., Gotfryd K., Chandra R., Goren M.A., Kruse A.C., Nurva S., Loland C.J., Pierre Y., Drew D., Popot J.L. Picot D., Fox B.G., Guan L., Gether U., Byrne B., Kobilka B., Gellman S.H., Maltose-neopentyl glycol (MNG) amphiphiles for solubilization, stabilization and crystallization of membrane proteins. Nature Methods (2010) 7:1003-8

• Protein expression (eg. baculovirus-mediated expression in insect and mammalian cell cultures, bacterial expression)
• Biochemistry (purification, reconstitution, Western blotting, analytical SEC, Co-IP, site-directed fluorescent labeling, site-directed cleavage, cross-linking, preparation of Fab fragments, scFv and nanobodies etc.)
• Molecular pharmacology (radioligand saturation and competition binding, various radioactivity-based G protein coupling assays, uptake assays for radiolabeled substrates)
• Molecular biology (plasmid preparation, site-directed mutagenesis, subcloning etc.)
• Immunocytochemistry
• Fluorescence spectroscopy
• Protein crystallography and cryoEM

Peer review

41 Nielsen A.K., Möller I.R., Wang Y., Rasmussen S.G.F. , Lindorff-Larsen K., Rand K.D., Loland C.J., Substrate-induced conformational dynamics of the dopamine transporter. Nature Communications (2019) 10:2714. DOI: 10.1038/s41467-019-10449-w

40 Du Y.§, Duc N.M.§, Rasmussen S.G.F. §, Hilger D., Kubiak. X., Wang L., Bohon J., Kim H.R., Wegrecki M., Asuru A., Jeong K.M., Lee J., Chance. M.R., Lodowski D.T., Kobilka B.K., Chung K.Y., Assembly of a GPCR-G protein complex. Cell (2019) 177:1232-1242.
§Authors contributed equally to this work

39 Möller I.R., Slivacka M., Nielsen A.K., Rasmussen S.G.F. , Gether U., Loland C.J., Rand K.D., Conformational Dynamics of the Human Serotonin Transporter during Substrate and Drug Binding. Nature Communications (2019) 10:1687. DOI:10.1038/s41467-019-09675-z

38 Walsh S.M., Mathiasen S., Christensen S.M., Fay J.F., King C., Provasi D., Borrero E., Rasmussen S.G.F. , Fung J.J., Filizola M., Hristova K.,Kobilka B. Farrens D.L., Stamou D., Single proteoliposome high content analysis reveals differences in the homo-oligomerization of class A GPCRs. Biophys. J. (2018) 115:300-312

37 McMahon C., Baier A.S., Pascolutti R., Wegrecki M., Zheng S., Ong J.X., Erlandson S.C., Hilger D., Rasmussen S.G.F. , Ring A.M., Manglik A., Kruse A.C., Yeast surface display platform for rapid discovery of conformationally selective nanobodies. Nature Structural and Molecular Biology (2018) 25:289-296

36 Boyhus L.E., Danielsen M., Bengtson N.S., Kunze M.B.A., Kubiak X., Sminia T.J., Løper J.H., Tran P.T., Lindorff-Larsen K., Rasmussen S.G.F. , Mathiesen J.M., Pedersen D.S., Gs protein peptidomimetics as allosteric modulators of the β2-adrenergic receptor, RSC Advances (2018) 8:2219-2238

35 Clark L., Dikiy I., Chapman K., Rödström K.E.J., Aramini J., LeVine M.V., Khelashvili G., Rasmussen S.G.F. , Gardner K.H., Rosenbaum D.M., Ligand modulation of sidechain dynamics in a wild-type human GPCR, Elife (2017), DOI: 10.7554/eLife.28505

34 DeVree B.T., Mahoney J.P., Vélez-Ruiz G.A., Rasmussen S.G.F. , Kuszak A.J., Edwald E., Fung J.J., Manglik A., Masureel M., Du Y., Matt R.A., Pardon E., Steyaert J., Kobilka B.K. and Sunahara R.K., Allosteric coupling from G protein to the agonist binding pocket in GPCRs. Nature (2016) 535:182-6

33 Mathiesen S., Christensen S.M., Fung J.J., Rasmussen S.G.F. , Fay J.F., Jørgensen S.K., Farrens D.L., Byrne M., Kobilka B., Stamou D., High-content analysis of single proteoliposome composition used to assay quantitatively GPCR oligomerization. Nature Methods (2014) 11:931-4

32 Pardon E., Laeremans T., Triest S., Rasmussen S.G.F. , Wohlkonig A., Ruf A., Muyldermans S., Hol W., Kobilks B.K., Steyaert J. A General Protocol for Generation of Nanobodies for Structural Biology. Nature Protocols (2014) 9:674-693

31 Staus D., Wingler L.M., Strachan R.T., Rasmussen S.G.F. , Pardon E., Ahn S., Steyaert J., Kobilka B., Lefkowitz R.J., Regulation of β2‐Adrenergic Receptor‐2 Function by Conformationally Selective Single‐domain Intrabodies. Mol. Pharmacol. (2014) 85:472-481

30 Chae P.S., Kruse A.C., Gotfryd K., Rana R.R., Rasmussen S.G.F. , Cho K.H., Bae E., Guan L., Loland C.J., Kobilka B., Byrne B., Gellman S.H. Novel tripod amphiphiles for membrane protein analysis. Chem. Eur. J. (2013) 19:15645-15651

29 Irannejad R., Tomshine J.C., Tomshine J.R., Chevalier M., Mahoney J.P., Steyaert J., Rasmussen S.G.F. , Sunahara R.K., El-Samad H., Huang B., von Zastrow M. Conformational biosensors reveal GPCR signaling from endosomes, Nature (2013) 495:534-538

28 Chae P.S., Rana R.R., Gotfryd K., Rasmussen S.G.F. , Kruse A.C., Cho K.H., Capaldi S., Kobilka B., Loland C.J., Gether U., Banerjee S., Byrne B., Lee J., Gellman S.H. Glucose-Neopentyl Glycol (GNG) amphiphiles for Membrane Protein Solubilization, Stabilization and Crystallization. ChemCommun (2013) 49:2287-89

27 Zocher M., Zhang C., Rasmussen S.G.F. , Kobilka B.K., Müller D.J., Cholesterol increases the kinetic, energetic, and mechanical stability of the human β2 adrenergic receptor. Proc. Natl. Acad. Sci. USA. (2012) 109:3463-72

26 Chae P.S., Rasmussen S.G.F. , Rana R.R., Gotfryd K., Kruse A.C., Nurva S., Gether U.,Guan L., Loland C.J., Byrne B., Kobilka B., Gellman S.H. A New Class of Amphiphiles Bearing Rigid Hydrophobic Groups for Solubilization and Stabilization of Membrane Proteins. Chem. Eur. J. (2012)

25 Chung K.Y§, Rasmussen S.G.F. §, Liu T. §, Li S., DeVree B.T., Chae P.S., Calinski D., Kobilka B.K., Woods V.L., Sunahara R.K. β2 Adrenergic Receptor-Induced conformational changes in the heterotrimeric G protein. Nature (2011) 477: 611-615.
§Authors contributed equally to this work

24 Westfield G.§, Rasmussen S.G.F. §, Su M. §, Dutta S. §, DeVree B.T., Chung K.Y.,Calinski D., Velez-Ruiz G., Oleskie A., Pardon E., Chae P.S., Liu T., Li. S., Woods V.L., Steyeart J., Kobilka B.K., Sunahara R.K., Skiniotis G., Structural Rearrangements of the Gαs α-Helical Domain in the β2-adrenoceptor Gs Complex. Proc. Natl. Acad. Sci. USA. (2011) 108:16086-91.
§Authors contributed equally to this work

23 Rasmussen S.G.F. §, DeVree B.T. §, Zho Y., Kruse A.C., Chung K.Y., Kobilka T.S., Thian F.S., Chae P.S., Pardon E., Calinski D., Mathiesen J.M., Shah S.T.A., Lyons J.A., Caffrey M., Gellman S.H., Steyaert J., Skiniotis G., Weis W.I., Sunahara R.K., Kobilka B.K., Crystal Structure of the β2 Adrenergic Receptor-Gs Protein Complex. Nature (2011) 477:550-555.
§Authors contributed equally to this work

22 Selao T.T., Branca R. , Chae P.S., Lehtiö J., Gellman S.H., Rasmussen S.G.F. , Nordlund S., Norén A., Identification of the chromophore membrane protein complex formed under different nitrogen availability conditions in Rhodospirilium rubrum. J. Prot. Res. (2011) 10:2703-2714

21 Rosenbaum D.M., Zhang C., Lyons J., Holl R., Aragao D., Arlow D.H., Rasmussen S.G.F. , Choi H.J., DeVree B.T., Sunahara R.K., Chae P.S., Gellman S.H., Dror R.O., Shaw D.E., Weis W.I., Caffrey M., Gmeiner P., Kobilka B.K., Structure and Function of an Irreversible Agonist- β2 Adrenoceptor Complex, Nature (2011) 469: 236-240

20 Rasmussen S.G.F. §, Choi H.J. §, Fung J.J.§, Casarosa P., Pardon E., Chae P.S., DeVree B.T., Rosenbaum D.M., Kobilka T.S., Thian F.S., Schnapp A., Konetzki I., Sunahara R.K., Gellman S.H., Pautsch A., Steyaert J., Weis W.I., Kobilka B.K., Structure of an Nanobody-Stabilized Active State of the β2 Adrenoceptor, Nature (2011) 469:175-180.
§Authors contributed equally to this work

19 Chae P.S., Gotfryd K., Pecyna J., Miercke L.J.W., Rasmussen S.G.F. , Robins R.A., Loland C.J., Kobilka B., Stroud R., Byrne B., Gether U., Gellman S.H. Tandem Facial Amphiphiles for Membrane Protein Stabilization. J. Am. Chem. Soc. (2010) 132:16750-16752

18 Chae P.S., Rasmussen S.G.F. , Rana R., Gotfryd K., Chandra R., Goren M.A., Kruse A.C., Nurva S., Loland C.J., Pierre Y., Drew D., Popot J.L. Picot D., Fox B.G., Guan L., Gether U.,Byrne B., Kobilka B., Gellman S.H., Maltose-neopentyl glycol (MNG) amphiphiles for solubilization, stabilization and crystallization of membrane proteins. Nature Methods (2010) 7:1003-8

17 Bokoch M.P., Zou Y., Rasmussen S.G.F. , Liu C.W., Nygaard R., Rosenbaum D.M., Fung J.J., Choi H.J., Thian F.S., Kobilka T.S., Puglisi J.D., Weis W.I., Pardo L., Prossor R.S., Mueller L., and Kobilka B.K., Ligand-specific regulation of the extracellular surface of a G protein coupled receptor. Nature (2010) 463:108-112

16 Eriksen J.§, Rasmussen S.G.F. §, Rasmussen T.N., Vaegter C.B., Cha J.H., Zhu M.F., Fog J.U., J.H., Newman A.H., Gether U., Visualization of dopamine transporter trafficking in live neurons by use of fluorescent cocaine analogues. J. Neuroscience (2009) 29:6794-808.
§Authors contributed equally to this work

15 Yao Y.J., Whorton M.R., Vélez-Ruiz G., Rasmussen S.G.F. , DeVree B.T., Deupi X., Sunahara R.K., and Kobilka B.K., The effect of ligand efficacy on the formation and stability of a GPCR-G protein complex. Proc. Natl. Acad. Sci. USA. (2009) 106:9501-9506

14 Richter W., Day P.W., Agrawal R., Bruss M., Granier S., Wang Y., Rasmussen S.G.F. , Horner K., Wang P., Lei T., Patterson A.J., Kobilka B., and Conti M., Signaling from β1- and β2-adrenergic receptors is defined by differential interactions with PDE4. EMBO J. (2008)27:384-393

13 Cherezov V., Rosenbaum D.M., Hanson M.A., Rasmussen S.G.F. , Thian F.S, Kobilka T.S, Choi H.J., Kuhn P., Weis W.I., Kobilka B.K, Stevens R.C, High Resolution Crystal Structure of a Modified Human β2-Adrenergic G protein-Coupled Receptor, Science (2007) 318:1258-1265

12 Rosenbaum D.M., Cherezov V., Hanson M.A., Rasmussen S.G.F. , Thian F.S., Kobilka T.S., Choi H.J., Yao Y.J., Kuhn P., Weis W.I., Stevens R.C., Kobilka B.K, GPCR Engineering Yields High-resolution Structural Insights into β2 Adrenergic Receptor Function, Science (2007) 318:1266-1273

11 Rasmussen S.G.F. §, Choi H.J. §, Rosenbaum D.M. §, Kobilka T.S., Thian F.S., Edwards P., Burghammer M., Ratnala V.R.P., Sanishvili R., Fischetti R., Schertler G.F.X, Weis W.I., Kobilka B.K., Crystal structure of the human β2 adrenergic G-protein-coupled receptor, Nature (2007) 450:383-387.
§Authors contributed equally to this work

10 Day P.W.§, Rasmussen S.G.F. §, Parnot C., Fung J.J., Masood A., Kobilka T.S., Yao Y.J., Choi H.J., Weis W.I., Rohrer D.K., Kobilka B.K., A monoclonal antibody for G protein coupled receptor crystallography, Nature Methods (2007) 4:927-929.
§Authors contributed equally to this work.

9 Plenge P., Gether U., and Rasmussen S.G.F. , Allosteric effects of R- and S-citalopram on the human 5-HT transporter: Evidence for distinct high- and low-affinity binding sites. Eur. J. Pharmacol. (2007) 567:1-9

8 Whorton M.R., Bokoch M.P., Rasmussen S.G.F. , Huang B., Zare R.N., Kobilka B., and Sunahara R.K. A monomeric G protein coupled receptor isolated in a high-density lipoprotein particle efficiently activates its G protein. Proc. Natl. Acad. Sci. USA. (2007) 104:7682-7687

7 Cha J.H., Zhu M.F., Adkins E.M., Rasmussen S.G.F. , Loland C.J., Schonenberger B., Gether U., Newman A.H., Rhodamine-Labeled 2b-Carbomethoxy-3b-(3,4-dichlorophenyl)tropane Analogues as High-Affinity Fluorescent Probes for the Dopamine Transporter, J. Med. Chem. (2005) 48:7513-7516

6 Rasmussen S.G.F. § and Gether U.§, Purification and Fluorescent Labeling of the Human Serotonin Transporter, Biochemistry (2005) 44: 3494-3505
§Authors share correspondence.

5 Ballesteros J.A. §, Jensen A.D.§, Liapakis G. §, Rasmussen S.G.F. §, Shi L., Gether U., Javitch J.A., Activation of the β2 Adrenergic Receptor Involves Disruption of an Ionic Lock Between the Cytoplasmic Ends of Transmembrane Segments 3 and 6, J. Biol. Chem. (2001) 276:29171-29177.
§Authors contributed equally to this work.

4 Jensen A.D., Guarnieri F., Rasmussen S.G.F. , Asmar F., Ballesteros, J.A., Gether U, Agonist-Induced Conformational Changes at the Cytoplasmic Side of TM6 in the β2 Adrenergic Receptor Mapped by Site-Selective Fluorescent Labeling, J. Biol. Chem. (2001) 276: 9279-9290.

3 Rasmussen S.G.F. , Carroll F.I., Maresch M., Jensen A.D., Tate C.G., Gether U.,Biophysical Characterization of the Cocaine Binding Pocket in the Serotonin Transporter Using a Fluorescent Cocaine Analogue as a Molecular Reporter, J. Biol. Chem. (2001) 276: 4717-4723.

2 Ghanouni P., Schambye H., Seifert R., Lee T.W., Rasmussen S.G.F. , Gether U., Kobilka B. K., The Effect of pH on β2 Adrenoceptor Function: Evidence for protonation-dependent activation, J. Biol. Chem. (2000) 275: 3121-3127.

1 Rasmussen S.G.F. , Jensen A.D., Liapakis G., Ghanouni P., Javitch J.A., Gether U., Mutation of a Highly Conserved Aspartic Acid in the β2 Adrenergic Receptor: Constitutive Activation, Structural Instability and Conformational Rearrangement of Transmembrane Segment 6, Mol. Pharmacol. (1999), 56: 175-184

Reviews

3 Rosenbaum D.M. Rasmussen S.G.F. , and Kobilka B.K, Structural and mechanistic insights into G-protein coupled receptor function, Nature (2009) 459:356-363

2 Rasmussen S.G.F. , Adkins E.M., Carroll F.I., Maresch M., Gether U. Structural and Functional Probing of the Biogenic Amine Transporters by Fluorescence Spectroscopy, Eur. J. Pharmacol., (2003) 479: 13-22.

1 Gether U., Asmar F., Meinild A.K., Rasmussen S.G.F. Structural basis for G-protein coupled receptor activation, Pharmacology and Toxicology (2002) 91: 304-312.

Chapters

5 Kuszak A.J., Yao X.J., Rasmussen S.G.F. , Kobilka B.K., and Sunahara R.K., Functional studies of isolated GPCR-G protein complexes in the membrane bilayer of lipoprotein particles, In G Protein-Coupled Receptors (eds. S. Siehler and and G. Milligan), ISBN-978-0-521-11208-6, Cambridge University Press, pp.32-52 (2010)

4 Weis W.I., Rosenbaum D.M., Rasmussen S.G.F. , et. al. Crystal Structures of the β2-Adrenergic Receptor, In From Molecules to Medicines, Structure of Biological Macromolecules and Its Relevance in Combating New Diseases and Bioterrorism(eds. J.L. Sussman and P. Spadon) ISBN: 978-90-481-2338-4, Springer Netherlands, pp. 217-230 (2009)

3 Rosenbaum D.M. Rasmussen S.G.F. , and Kobilka B.K, The β2 Adrenergic Receptor as a Model for G Protein-Coupled Receptor Structure and Activation by Diffusible Hormones, In The Handbook of Cell Signaling (eds. R. Bradshaw and E. Dennis), ISBN-10: 0-12-374145-9, Elsevier Inc., (2009)

2 Rasmussen S.G.F. and Gether U., Molecular mechanisms of GPCR activation, In G Protein-Coupled Receptors as Drug Targets: Analysis of Activation and Constitutive Activity, Volume 24 (eds. R. Seifert and T. Wieland), ISBN: 3-527-30819-9, WILEY-VCH Verlag GmbH, Weinheim, pp. 27-42 (2006)

1 Rasmussen S.G.F. and Gether U., Structural mechanics of GPCR activation, In Understanding G-protein coupled receptors and their role in the CNS (eds. M.N. Pangalos and C.H. Davies), ISBN: 0-19-850916-2, Oxford University Press, Oxford: New York, pp. 43-62 (2002)