Perrier Lab

Our lab has a developed a strong expertise in the study of intrinsic properties of individual neurons.

Jean-Francois Perrier info

 

 

 

 

 

Credit: Gil Costa

Role of glial cells in motor control

We are investigating how glial cells from the spinal cord contribute to motor control. We discovered that astrocytes from the ventral horn of the spinal cord respond to endocannabinoids released from active neighbor interneurons. In turn, astrocytes release purines which inhibit the release of excitatory neurotransmitters. This cellular mechanism acts as a filter that prevents motor tremor. It also explains in part the anti-tremor effect of cannabis reported by patients suffering from neurodegenerative diseases. See Carlsen et al., Nature Neuroscience 2021.



Physiological function of neuronal intrinsic properties

Our lab has a developed a strong expertise in the study of intrinsic properties of individual neurons. By means of technique such as patch clamp recording, pharmacology, two-photon imaging in slice preparations from the central nervous, we are investigating how ion channels generate the electrical activity of neurons and how this contributes to the behavior of neural networks. Our recent results include:

- The identification of the first cellular mechanism responsible for the motor fatigue that occurs in the central nervous system. Motoneurons are the final common output of the central nervous system. Their synaptic activation triggers the contraction of the muscle they innervate. Neurons from the raphe nuclei in the brainstem release serotonin on motoneurons by means of synaptic contacts. Their activation facilitates the activity of motoneurons and thereby muscle contraction. During intense physical activity, serotonin release is increased. A spillover occurs and serotonin reaches extrasynaptic receptors located on the axon initial segment of motoneurons. This inhibits action potential genesis and thereby muscle contraction. In that way serotonin prevents excessive muscle contraction (Cotel et al., 2013).

                     






- The discovery of a new pathway that could prevent the development of temporal lobe epilepsy. The subiculum is a part of the temporal lope of the brain, and this is where temporal lobe epilepsy originates. Calcium flowing through CaV3 channels is responsible for the bursts of activity by pyramidal cells in the subiculum. When the activity of the neurons becomes overly synchronous, it results in abnormal electrical fluctuations, which lead to epileptic seizures. We have found that the activation of serotonin 2C receptors decreases the level of bursting by inhibiting CaV3 channels. This discovery could lead to the development of new principles for treating temporal lobe epilepsy (Petersen et al., 2017). See our popularization article in Videnskab.dk

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Lab members

Name Title Job responsibilities Image
Alexia Montalant PhD Student Perrier lab Billede af Alexia Montalant
Altair Brito dos Santos Postdoc Perrier lab Billede af Altair Brito dos Santos
Anne Marie Nordvig Petersen Laboratory Technician Perrier Lab Billede af Anne Marie Nordvig Petersen
Christian Laut Ebbesen International Researcher Perrier Lab Billede af Christian Laut Ebbesen
Jean-Francois Marie Perrier Associate Professor Perrier lab Billede af Jean-Francois Marie Perrier
Jørn Dybkjær Hounsgaard No job title VIP Perrier Lab Billede af Jørn Dybkjær Hounsgaard
Liangchen Guo Academic Research Staff Billede af Liangchen Guo
Nikolaj Winther Hansen PhD Student Perrier lab Billede af Nikolaj Winther Hansen
Silas Dalum Larsen Laboratory Assistant Perrier Lab Billede af Silas Dalum Larsen
Thor Linnet Academic Research Staff Billede af Thor Linnet

Collaborations