MacAulay Lab
The research group focuses on elucidation of the molecular mechanisms underlying water and ion homeostasis in the mammalian brain under both physiological and pathophysiological conditions.
Researchers from the University of Copenhagen have discovered that fluid does not necessarily enter the brain the way one thought. According to one of the researchers behind the study, the result may lead to fewer major brain operations.
Read the full article here.
Now we are one (small) step closer to our vision of obtaining medicine for conditions of elevated brain water content and thus elevated intracranial pressure.
Hydrocephalus (too much water in the head) occurs as a consequence of brain bleeds – either in neonatal babies or in the general population following trauma, ruptured aneurisms, or the like.
It has generally been assumed that the water accumulation occurred following haemorrhage-mediated blockage of the brain fluid outflow. But here we demonstrate that some fraction of the fluid build-up originate from hypersecretion of the brain fluid that surrounds our brain and fills the central cavities.
The inflammatory conditions arising with the hemorrhagic event cause hyper-activity of the transporters in choroid plexus – and thereby elevate brain fluid secretion (see verses in the post or find the full text link in commentarys, if you are interested).
Transporter = protein = potential pharmacological target. We do need more – and better - of those! …..and will keep looking!
Thanks to all my wonderful co-workers @ and thanks to IMK Almene Fond and @novo for their support to this study.
An increasing number of elderly patients are diagnosed with a form of hydrocephalus that does not appear to cause elevated intracranial pressure – therefore the ‘normal pressure’ hydrocephalus. It comes across as an oxymoron (a useful word meaning ‘apparently contradictory terms that appear in conjunction’) that one can have enlarged ventricles an no elevation of pressure. ‘Idiopathic’ means that the cause is unknown.
We tested the hypothesis that this patient group has elevated osmolarity of their brain fluid (cerebrospinal fluid = CSF) – which they did not! (read the short summary on verse or find the article here).
So the search continues. We need to know how the disease arises to be able to target a precise mechanism in future treatment options.
Thanks to all the patients who permitted us to use their cerebrospinal fluid that was sampled during the diagnostic work up and thanks to Novo Nordisk foundation for supporting the study.
Great to see all our wonderful NeuroGrads F2F again for the ‘fun part’ of our annual NeuroGrad Winter School. Thanks to 2021 Brain Prize winner Jes Olesen for taking us through his path from PhD student to winner of the most prestigious brain research prize, to Jan Hellesøe for showing us a different perspective of ‘fiddling with the brain’ (compared to patient/bench work), and thanks to all students and supervisors participating.
Crazy – isn’t it? A small tissue, with the complicated name choroid plexus, resides deep in our brains and secretes the 500 ml of fluid that daily enters our brain. But we know little about the transporters expressed in this tissue.
Here we identify, sort, and list the transporters, channels, receptors, and signaling molecules expressed in our very favorite tissue. Check out whether your favorite protein is amongst these either in the paper or in the associated database.
Great work Søren Norge Andreassen – and colleagues Trine L. Toft, Jonathan H. Wardman, and Rene Villadsen – and thanks to the Lundbeck Foundation for funding the project.
Nanna MacAulay researches the large quantity of water in the mammalian brain which is continuously shifted between the circulating blood and the brain parenchyma as well as between different compartments and cellular structures within the brain tissue. One presumes that the transport of water between these different compartments is under tight control since a disturbance in the cerebral water homeostasis (with associated changes in ion concentrations) may lead to neuronal dysfunction, hydrocephalus, and/or brain edema. However, the incomplete knowledge of the molecular mechanisms responsible for the maintenance of cerebral water transport and their regulation currently prevents the research field from gaining a full understanding of this intricate and crucial (patho)physiological issue. With this lack of identification of the implicated transport mechanisms and their dysregulation in pathology, pharmacological therapy is essentially unavailable for potentially life-threatening conditions involving brain water accumulation, i.e. hydrocephalus, brain edema, acute liver failure, idiopathic intracranial hypertension, etc.
The research group focuses on elucidation of the molecular mechanisms underlying water and ion homeostasis in the mammalian brain under both physiological and pathophysiological conditions. More specifically, the laboratory investigates the transport mechanisms underlying cerebrospinal fluid secretion, brain extracellular fluid generation, activity-dependent glial cell swelling during stimulus-evoked K+ management, and dendritic beading observed during spreading depolarization. The technical approach spans from molecular and biophysical properties of water transport proteins (including aquaporins and cotransporters) to their regulation at the cellular level and their integral function in acutely prepared brain slices and rodent in vivo models.
- Nanna MacAulay(2021) Molecular mechanisms of brain water transport. Nature Reviews Neuroscience 6:326-344
- Trine Lisberg Toft-Bertelsen, Brian Roland Larsen, Sofie Kjellerup Christensen, Himanshu Khandelia, Helle S. Waagepetersen & Nanna MacAulay (2020) Clearance of activity-evoked K+ transients and associated glia cell swelling occur independently of AQP4; A study with an isoform-selective AQP4 inhibitor.GLIA 69: 28-41
- Lykke K, Assentoft M, Hørlyck S, Helms HCC, Stoica A, Toft-Bertelsen TL, Tritsaris K, Vilhardt F, Brodin B & MacAulay N (2019) Evaluating the involvement of cerebral microvascular endothelial Na+/K+-ATPase and Na+-K+-2Cl- co-transporter (NKCC1) in electrolyte fluxes in an in vitro BBB model of dehydration. Journal of Cerebral Blood Flow and Metabolism 39: 497-512
- Eva Kjer Oernbo, Kasper Lykke, Annette B. Steffensen, Kathrin Töllner, Christina Kruuse, Martin Fredensborg Rath, Wolfgang Löscher & Nanna MacAulay (2018) Cerebral influx of Na+ and Cl- as the osmotherapy-mediated rebound response in rats. Fluids and Barriers in the CNS 15:27
- Steffensen AB, Oernbo EK, Stoica S, Gerkau NJ, Barbuskaite D, Tritsaris K, Rose CR & MacAulay N (2018) Cotransporter-mediated water transport underlying cerebrospinal fluid formation. Nature Communications 9:2167
- Larsen BR & MacAulay N (2017) Activity-dependent astrocyte swelling is mediated by pH-regulating mechanisms. GLIA 65:1668-1681
- Toft-Bertelsen TL, Krízaj D & MacAulay N (2017) When size matters: transient receptor potential vanilloid 4 channel as a volume-sensor rather than an osmo-sensor. Journal of Physiology 595:3287-3302
- Steffensen AB, Sword J, Croom D, Kirov SA & MacAulay N (2015) Cotransporters as a molecular mechanism underlying spreading depolarization-induced dendritic beading. Journal of Neuroscience 35:12172-12187
- Larsen BR, Assentoft A, Cotrina ML, Hua SZ, Nedergaard M, Kaila K, Voipio J & MacAulay N (2014) Contributions of the Na+/K+-ATPase, NKCC1, and Kir4.1 to hippocampal K+-clearance and volume responses. GLIA 62:608-622
Born 15th December 1972
Education
2020 DMSci from UCPH
2002 PhD from UCPH
1998 MSc in biochemistry from McGill University, Canada
1995-1996 Student exchange to McGill University, Canada
1995 BSc in biology from UCPH
Positions
2019- Professor at Department of Neuroscience UCPH
2015-2018 Associate professor at Department of Neuroscience UCPH
2006-2015 Associate professor at Department of Cellular and Molecular Medicine, UCPH
2004-2006 Associate professor at Department of Medical Physiology, UCPH
2002-2003 Postdoc at Department of Medical Physiology, UCPH
Awards and honors
2019 Lundbeck foundation’s Ascending Investigator Grant
2019 Leo Danin’s Research award
2017 NNF’ tandem program grant
2012 Danish Independent Research Council’s career programme ‘Sapere Aude’
2008 L’Oreal, Unesco and the Royal Danish Academy of Sciences’ scholarship to ‘Young Women in Natural Sciences’
2006 Danish Independent Research Council’s ’Young Elite Researcher’s Award’
Commissions of trust
2019 – Panel member British Royal Society International Exchanges
2019 – Associate Editor, Fluids and Barriers in the CNS (editorial board member 2016-)
2017-2021 Member of departmental management team at Department of Neuroscience
2016- Head of Graduate Programme in Neuroscience, NeuroGrad
2016- Organizer of the Neuroseminars
2013 – Editorial Board Member, GLIA
2013-2016 Co-chair of the Society of Physiology’s special interest group “Molecular physiology of channels and transporters’
2011-2016 Member of the strategic research committee at the Faculty of Health, UCPH
2008-2015 Member of the research committee at Dept of Cellular and Molecular Medicine, UCPH
Grants last 10 years
2020-2023 Independent Research Fund Denmark: 2.5 MDKK
2020-2024 Lundbeck Foundation Ascending investigator grant: 5 MDKK
2020-2021 Carlsberg Foundation: 0.3 MDKK
2019-2023 Lundbeck Foundation Thematic Grant (co-applicant, my share): 4.4 MDKK
2019-2021 NovoNordisk Foundation Symposium: 0.25 MDKK
2018-2022 IMK Almene Foundation: 0.9 MDKK
2018-2022 Friis’ Foundation: 0.5 MDKK
2018-2019 Brdr. Hartmann’s Foundation: 0.5 MDKK
2017-2022 NovoNordisk Foundation – Tandem (main applicant, my share): 7.5 MDKK
2019-2020 National Institute of Health (co-investigator, my share): 0.15 MDKK
2016-2018 NovoNordisk Foundation: 1.2 MDKK
2016-2019 Augustinus Foundation: 0.3 MDKK
2016-2017 Michaelsen’s Foundation: 0.3 MDKK
2016-2017 Carslberg Foundation: 0.1 MDKK
2014-2017 Lundbeck Foundation: 1.6 MDKK
2014-2015 Friis’ Foundation: 0.3 MDKK
2013-2016 Thorberg’s Foundation: 2.5 MDKK
2012-2017 Independent Research Fund Denmark (Sapere Aude): 6.8 MDKK
2011-2014 Independent Research Fund Denmark 1.8 MDKK
2011-2014 Lundbeck Foundation: 0.7 MDKK
In vivo animal experimentation on anesthetized rodents (including artificial ventilation and measurements of blood gases in addition to determination of brain water and electrolyte content)
In vivo determination of cerebrospinal fluid production (ventriculo-cisternal perfusion and whole animal live imaging; Li-cor Biosciences’ Pearl small animal imaging)
Ion-sensitive microelectrode measurements of (K+, pH, extracellular space) in rodent brain slices
Micro-fluidic chip-based volume measurements of mammalian cells
Two-electrode voltage clamp of Xenopus oocytes (heterologously expressing transport proteins)
Volume measurements of Xenopus oocytes (heterologously expressing transport proteins)
Radio-isotope flux measurements (in ex vivo tissue, cell culture, Xenopus oocytes)
Molecular biology (plasmid preparation, mutagenesis, subcloning, sequencing, in vitro RNA transcription, etc.)
Western blot, surface biotinylation, immunostaining, etc.
2022
92. Trine L Toft-Bertelsen, Dagne Barbuskaite, Eva K Heerfordt, Sara D Lolansen, Søren N Andreassen, Nina Rostgaard, Markus H Olsen, Nicolas H. Norager, Tenna Capion, Martin F Rath, Marianne Juhler & Nanna MacAulay (2022) Lysophosphatidic acid as a CSF lipid in posthemorrhagic hydrocephalus that drives CSF accumulation via TRPV4-induced hyperactivation of NKCC1. Fluids and Barriers of the CNS In press
91. Eva K. Oernbo1, Annette B. Steffensen, Pooya R Khamesi, Trine L Toft-Bertelsen, Dagne Barbuskaite, Frederik Vilhardt, Niklas J. Gerkau, Katerina Tritsaris, Anja H Simonsen, Sara D Lolansen, Søren N Andreassen, Steen G. Hasselbalch, Thomas Zeuthen, Christine R. Rose, Vartan Kurtcuoglu & Nanna MacAulay (2022) Membrane transporters control cerebrospinal fluid formation independently of conventional osmosis to modulate intracranial pressure. Fluids and Barriers of the CNS In press
90. Sara D Lolansen, Nina Rostgaard, Dagne Barbuskaite, Tenna Capion, Markus H Olsen, Nicolas H Norager, Frederik Vilhardt, Søren N Andreassen, Trine L Toft-Bertelsen, F Ye, Marianne Juhler, Richard Keep & NannaMacAulay (2022) Posthemorrhagic hydrocephalus associates with elevated inflammation and CSF hypersecretion via activation of choroidal transporters. Fluids and Barriers of the CNS 19:62
89. Dagne Barbuskaite, Eva K Oernbo, Jonathan H Wardman, Trine L Toft-Bertelsen, Eller Conti E, Søren N Andreassen, Niklas J Gerkau, Christine R. Rose & NannaMacAulay (2022) Acetazolamide modulates intracranial pressure directly by its action on the cerebrospinal fluid secretion apparatus. Fluids and Barriers of the CNS 19:53
88. Eva K.Oernbo, Annette B. Steffensen, Hanne Gredal, Helle H. Poulsen, Nina Rostgaard, CH Rasmussen, M Møller-Nissen, Anja H. Simonsen, Steen G. Hasselbalch, Marianne Juhler & Nanna MacAulay (2022) Cerebrospinal fluid osmolality cannot predict development or surgical outcome of idiopathic normal pressure hydrocephalus. Fluids and Barriers of the CNS 19:52
87. Søren N. Andreassen, Trine L. Toft-Bertelsen, Jonathan H. Wardman, Rene Villadsen & NannaMacAulay (2022) Transcriptional profiling of transport mechanisms and regulatory pathways in rat choroid plexus. Fluids and Barriers of the CNS 19:44
86. Marianne Juhler, TS Hansen, HVG Novrup, NannaMacAulay & Tina N. Munch (2022) Hydrocephalus Study Design: Testing New Hypotheses in Clinical Studies and Bench-to-Bedside Research. World Neurosurgery 161:424-431
85. Nanna MacAulay, Richard F. Keep & Thomas Zeuthen (2022) Cerebrospinal fluid production by the choroid plexus: a century of barrier research revisited. Fluids and Barriers of the CNS 19:26
2021
84. Sara Diana Lolansen, Nina Rostgaard, Søren Norge Andreassen, Anja Hviid Simonsen, Marianne Juhler, Steen Gregers Hasselbalch and Nanna MacAulay (2021) Elevated CSF inflammatory markers in patients with idiopathic normal pressure hydrocephalus do not promote NKCC1 hyperactivity in rat choroid plexus. Fluids and Barriers of CNS, accepted for publication
83. Monica Lakk, GF Hoffmann, A. Gorusupudi, E Enyong, A Lin, PS Bernstein, Trine L. Toft-Bertelsen, Nanna MacAulay, MH Elliott, David Križaj (2021) Membrane cholesterol regulates TRPV4 function, cytoskeletal expression, and the cellular response to tension. Journal of Lipid Research:100145, online ahead of print
82. Trine L. Toft-Bertelsen & Nanna MacAulay (2021) TRPing on Cell Swelling - TRPV4 Senses It. Frontiers of Immunology 12:730982
81. Nanna MacAulay (2021) Reply to ‘Aquaporin 4 and glymphatic flow have central roles in brain fluid homeostasis’. Nature Reviews Neuroscience 10:651-652
80. Nanna MacAulay(2021) Molecular mechanisms of brain water transport. Nature Reviews Neuroscience 6:326-344
79. Sara D. Lolansen, Nina Rostgaard, Eva Kjer Oernbo, Marianne Juhler, Anja Hviid Simonsen & Nanna MacAulay (2021). Inflammatory markers in cerebrospinal fluid from patients with hydrocephalus: a systematic literature review. Disease Markers 8834822. doi: 10.1155/2021/8834822
78. Trine L. Toft-Bertelsen & Nanna MacAulay (2021) TRPing to the Point of Clarity: Understanding the Function of the Complex TRPV4 Ion Channel. Cells 10:165. doi: 10.3390/cells10010165
2020
77. G.V. Mkrtchyan, K. Abdelmohsen, P. Andreux et al. (2020) ARDD 2020: from aging mechanisms to interventions. Aging 12:24484-24503
76. Nanna MacAulay & Christine C. Rose (2020) Rebuttal from Nanna MacAulay and Christine C. Rose. Journal of Physiology 598:4743
75. Nanna MacAulay & Christine C. Rose (2020) CrossTalk opposing view: NKCC1 in the luminal membrane of choroid plexus is outwardly directed under basal conditions and contributes directly to cerebrospinal fluid secretion. Journal of Physiology 598: 4737-4739
74. Trine Lisberg Toft-Bertelsen, Brian Roland Larsen, Sofie Kjellerup Christensen, Himanshu Khandelia, Helle S. Waagepetersen & Nanna MacAulay (2020) Clearance of activity-evoked K+ transients and associated glia cell swelling occur independently of AQP4; A study with an isoform-selective AQP4 inhibitor. GLIA 69: 28-41
73. Nanna MacAulay (2020) Molecular mechanisms of K+ clearance and extracellular space shrinkage-Glia cells as the stars. GLIA 68:2192-2211
72. Brian Skriver Nielsen, Trine Lisberg Toft-Bertelsen, Sara D. Lolansen, Connor L. Anderson, Morten Schak Nielsen, Roger J. Thompson & Nanna MacAulay (2020) Pannexin 1 activation and inhibition is permeant-selective. Journal of Physiology 598:361-379
2019
71. Trine Lisberg Toft-Bertelsen, Oleg Yarishkin, Sara Redmon,Tam TT Phuong, David Krizaj & Nanna MacAulay (2019) Volume sensing in the transient receptor potential vanilloid 4 ion channel is cell type-specific and mediated by an N-terminus volume-sensing domain. Journal of Biological Chemistry 294:18421-18434
70. Brian Skriver Nielsen, Francesco Zonta, Thomas Farkas, Thomas Litman, Morten Schak Nielsen & Nanna MacAulay (2019) Structural determinants underlying permeant discrimination of the Cx43 hemichannel. Journal of Biological Chemistry 294:16789-16803
69. Kasper Lykke, Mette Assentoft, Sofie Hørlyck, Hans Christian C. Helms, Anca Stoica, Trine L. Toft-Bertelsen, Katerina Tritsaris, Frederik Vilhardt, Birger Brodin & Nanna MacAulay (2019) Evaluating the involvement of cerebralmicrovascular endothelial Na+/K+-ATPase and Na+-K+-2Cl- co-transporter (NKCC1) in electrolyte fluxes in an in vitro BBB model of dehydration. Journal of Cerebral Blood Flow and Metabolism 39: 497-512.
68. Brian Roland Larsen, Anca Stoica, Nanna MacAulay (2019) Developmental maturation of activity-induced K+and pH transients and the associated extracellular space dynamics in the rat hippocampus. Journal of Physiology, 597: 583-597
2018
67. Claudia Brandt, P Seja, Katrin Töllner, Kerstin Römermann, Philip Hampel, M Kalesse, A Kipper, Peter W Feit, Kasper Lykke, Trine L Toft-Bertelsen, P Paavilainen, I Spoljaric, Martin Puskarjov , Nanna MacAulay, Kai Kaila, Wolfgang Löscher (2018) Bumepamine, a brain-permeant benzylamine derivative of bumetanide, does not inhibit NKCC1 but is more potent to enhance phenobarbital's anti-seizure efficacy. Neuropharmacology 143:186-204
66. Eva Kjer Oernbo, Kasper Lykke, Annette B. Steffensen, Kathrin Töllner, Christina Kruuse, Martin Fredensborg Rath, Wolfgang Löscher & Nanna MacAulay (2018) Cerebral influx of Na+ and Cl- as the osmotherapy-mediated rebound response in rats. Fluids and Barriers in the CNS 15:27
65. Philip Hampel, Kerstin Römermann, Nanna MacAulay, Wolfgang Löscher (2018) Azosemide is more potent than bumetanide and various other loop diuretics to inhibit the sodium-potassium-chloride-cotransporter human variants hNKCC1A and hNKCC1B. Scientific Reports 8:9877
64. Annette B. Steffensen, Eva K. Oernbo, Anca Stoica, Niklas J. Gerkau, Dagne Barbuskaite, Katerina Tritsaris, Christine R. Rose & Nanna MacAulay (2018) Cotransporter-mediated water transport underlying cerebrospinal fluid formation. Nature Communications 9:2167
63. Katja Stahl, S. Rahmani, A. Prydz, N. Skauli, Nanna MacAulay, M.N. Mylonakou, R. Torp, Ø. Skare, T. Berg, T.B. Leergaard, R.E. Paulsen, O.P. Ottersen, Mahmood Amiry-Moghaddam (2018) Targeted deletion of the aquaglyceroporin AQP9 is protective in a mouse model of Parkinson's disease. PLoS One 13:e0194896
62. Trine L. Toft-Bertelsen, Brian Roland Larsen & Nanna MacAulay (2018) Sensing and regulation of cell volume - we know so much and yet understand so little: TRPV4 as a sensor of volume changes but possibly without a volume-regulatory role? Channels 12;100-108
61. Siren Berland, Trine L. Toft-Bertelsen, Ingvild Aukrust, Jan Byska, Marc Vaudel, Laurence A. Bindoff, Nanna MacAulay (shared senior authorship) & Gunnar Houge (2018) A de novo Ser111Thr variant in aquaporin-4 in a patient with intellectual disability, transient signs of brain ischemia, transient cardiac hypertrophy, and progressive gait disturbance. Molecular Case Studies 4(1), pii: a002303
2017
60. Brian Skriver Nielsen, Jette Skov Alstrom,Bruce J. Nicholson, Morten Schak Nielsen & Nanna MacAulay (2017) Permeant-specific gating of connexin 30 hemichannels. Journal of Biological Chemistry 292:19999-20009
59. Lena Rosenbaek, Federica Rizzo, Qi Wu, Lorena Rojas-Vega, Gerardo Gamba Ayala, Nanna MacAulay, Oliver Staub & Robert A. Fenton (2017) The thiazide sensitive sodium chloride co-transporter NCC is modulated by site-specific ubiquitylation. Scientific
Reports 7(1):12981
58. Anca Stoica, Brian Roland Larsen, Mette Assentoft, Rikke Holm, Leanne M. Holt, Frederik Vilhardt, Bente Vilsen, Karin Lykke-Hartmann, Michelle L. Olsen & Nanna MacAulay (2017) The α2β2 isoform combination dominates the astrocytic Na+ /K+-ATPase activity and is rendered nonfunctional by the α2.G301R familial hemiplegic migraine type 2-associated mutation. GLIA 65:1777-1793
57. Brian Roland Larsen & Nanna MacAulay (2017) Activity-dependent astrocyte swelling is mediated by pH-regulating mechanisms. GLIA 65:1668-1681
56. Lena L. Rosenbaek. F. Rizzo, Nanna MacAulay, Olivier Staub & Robert A. Fenton (2017) Functional Assessment of Sodium Chloride Co-transporter NCC Mutants in Polarized Mammalian Epithelial Cells. American Journal of Physiology - Renal Physiology 313:F495-F504
55. Brian s. Nielsen, Daniel B. Hansen, Bruce R. Ransom, Morten S. Nielsen & Nanna MacAulay (2017) Connexin hemichannels in astrocytes: An assessment of controversies regarding their functional characteristics. Neurochemical
Research 42:2537-2550
54. Trine L. Toft-Bertelsen, David Krízaj & Nanna MacAulay (2017) When size matters: transient receptor potential vanilloid 4 channel as a volume-sensor rather than an osmo-sensor. Journal of Physiology 595:3287-3302
2016
53. Emma Olesen, Hanne Moeller, Mette Assentoft, Nanna MacAulay & Robert Fenton (2016) The Vasopressin Type-2 Receptor and Prostaglandin Receptors EP2 and EP4 can Increase Aquaporin-2 Plasma Membrane Targeting Through a cAMP Independent Pathway. American Journal of Physiology – Renal Physiology 311:935-944
52. Mette Assentoft, Shreyas Kaptan, Hans-Peter Schneider, Joachim W. Deitmer, Bert L. de Groot & Nanna MacAulay (2016) Aquaporin 4 as a NH3 channel. Journal of Biological Chemistry 291:19184-19195
51. P. Wanitchakool, J. Ousingsawat, L. Sirianant, Nanna MacAulay, Rainer Schreiber & Karl Kunzelmann (2016) Cl- channels in apoptosis. European Biophysical Journal 45: 599-610
50. Brian Roland Larsen, Rikke Holm, Bente Vilsen & Nanna MacAulay (2016) Glutamate transporter activity promotes enhanced Na+/K+-ATPase-mediated extracellular K+ management during neuronal activity. Journal of Physiology 594:6627-6641
49. Brian Roland Larsen, Anca Stoica & Nanna MacAulay (2016) Managing brain extracellular K+ during neuronal activity: The physiological role of the Na+/K+-ATPase subunit isoforms. Frontiers of Physiology 7(141), fphys.2016.00141
48. Kasper Lykke, Kathrin Töllner, Peter W. Feit, Thomas Erker, Nanna MacAulay & Wolfgang Löscher (2016) The search for NKCC1-selective drugs: Structure-function relationship of bumetanide and various bumetanide derivatives in inhibiting the human cation-chloride cotransporter NKCC1A. Epilepsy & Behavior 59:42-49
47. Nils Landegren, M. Pourmousa Lindberg, J. Skov, Å. Hallgren, D. Eriksson, Trine Lisberg Toft-Bertelsen, Nanna MacAulay, E. Hagforsen, A. Räisänen-Sokolowski, H. Saha, T. Nilsson, G. Nordmark, S. Ohlsson, J. Gustafsson, E.S. Husebye, E. Larsson, M.S. Anderson, J. Perheentupa, F. Rorsman, Robert A. Fenton & O. Kämpe (2016) Autoantibodies Targeting a Collecting Duct-Specific Water Channel in Tubulointerstitial Nephritis. Journal of American Society of Nephrology 27:3220-3228
46. Hanne B. Moeller, J. Slengerik-Hansen, T. Aroankins, Mette Assentoft, Nanna MacAulay, Søren K. Moestrup, V. Bhalla & Fenton RA (2016) Regulation of the Water Channel Aquaporin-2 via 14-3-3θ and -ζ. Journal of Biological Chemistry 291:2469-84
2015
45. Andrew Jo, Daniel A. Ryskamp, Tam T.T. Phuong, Alan S. Verkman, Oleg Yarishkin, Nanna MacAulay & David Križaj (2015). TRPV4 and AQP4 channels synergistically regulate cell volume and calcium homeostasis in retinal Müller glia. Journal of Neuroscience 35:13525-13537
44. Shreyas Kaptan, Mette Assentoft, Hans Peter Schneider, Robert A. Fenton, Joachim Deitmer, Nanna MacAulay & Bert de Groot (2015) Is H95 a pH-dependent gate in aquaporin 4? Structure 23:2309-2318
43. Jette S. Alstrom, Daniel B. Hansen, Morten S. Nielsen & Nanna MacAulay (2015) Isoform-specific phosphorylation-dependent regulation of connexin hemichannels. Journal of Neurophysiology 14:3014-22
42. Annette B. Steffensen, Jeremy Sword, Deborah Croom, Sergei A. Kirov & Nanna MacAulay (2015) Cotransporters as a molecular mechanism underlying spreading depolarization-induced dendritic beading. Journal of Neuroscience 35:12172-12187
41. Kasper Lykke, Mette Assentoft, Robert A. Fenton, Mette M. Rosenkilde & Nanna MacAulay (2015). Vasopressin receptors V1a and V2 are not osmo-sensors. Physiological Reports 3(8), phy2.12519
40. Kasper Lykke, Kathrin Töllner, Kerstin Römermann, Peter W. Feit, Thomas Erker, Nanna MacAulay & Wolfgang Löscher (2015) Structure-activity relationships of bumetanide derivatives: correlation between diuretic activity in dogs and inhibition of human NKCC2 variant A. British Journal of Pharmacology, bph.13231
39. Mette Assentoft, Brian Roland Larsen & Nanna MacAulay (2015) Regulation and Function of AQP4 in the Central Nervous System. Neurochemical Research 40:2615-2627
38. Martin N. Andersen, Lasse Skibsbye, C. Tang, F. Petersen, Nanna MacAulay, Hanne B. Rasmussen & Thomas Jespersen (2015) PKC and AMPK regulation of Kv1.5 potassium channels. Channels 9:121-8
37. Jette S. Alstrom, Line W. Stroemlund, Morten S. Nielsen & Nanna MacAulay (2015) Protein kinase C-dependent regulation of connexin43 gap junctions and hemichannels. Biochemical Society Transaction 43:519-23
36. Daniel Ryskamp, Andrew Jo, Amber Frye, Felix Vazquez-Chona, Nanna MacAulay, Wallace Thoreson, and David Krizaj (2015) Swelling and eicosanoid metabolites differentially gate TRPV4 channels in retinal neurons and glia. Journal of Neuroscience 34:15689-15700
2014
35. Brian Roland Larsen & Nanna MacAulay (2014) Kir4.1-mediated spatial buffering of K+: Experimental challenges in determination of its temporal and quantitative contribution to K+ clearance in the brain. Channels 8:544-560
34. Mette Assentoft, Brian Roland Larsen, Emma T.B. Olesen, Robert A. Fenton & Nanna MacAulay (2014) AQP4 plasma membrane trafficking or channel gating is not significantly modulated by phosphorylation at C-terminal serine residues. American Journal of Physiology – Cell Physiology 307:957-965
33. Daniel Bloch Hansen, Ye Zu-Cheng, Kirstine Calloe, Thomas H. Braunstein, Johannes P. Hofgaard, Bruce R. Ransom, Morten S. Nielsen & Nanna MacAulay (2014) Activation, permeability, and inhibition of astrocytic and neuronal large pore (hemi)channels. Journal of Biological Chemistry 289:26058-26073
32. Daniel Bloch Hansen, Thomas H. Braunstein, Morten S. Nielsen & Nanna MacAulay (2014) Distinct permeation profiles of the connexin 30 and 43 hemichannels. FEBS Letters 588:1446-1457
31. Brian Roland Larsen, Mette Assentoft, Maria L. Cotrina, Susan Z. Hua, Maiken Nedergaard, Kai Kaila, Juha Voipio & Nanna MacAulay (2014) Contributions of the Na+/K+-ATPase, NKCC1, and Kir4.1 to hippocampal K+-clearance and volume responses. GLIA 62:608-622
2013
30. Thomas Zeuthen, Magnus Alsterfjord, Eric Beitz & Nanna MacAulay (2013) Osmotic water transport in aquaporins. Evidence for a stochastic mechanism. Journal of Physiology 591:5017-5029
29. Rikke Sogaard, Lars Borre, Thomas H. Braunstein, Kenneth L. Madsen & Nanna MacAulay (2013) Functional modulation of the glutamate transporter variant GLT1b by the PDZ domain protein PICK1. Journal of Biological Chemistry 288:20195-20207
28. Mette Assentoft, Shreyas Kaptan, Robert A. Fenton, Susan Z. Hua, Bert L. de Groot & Nanna MacAulay (2013) Phosphorylation of rat aquaporin-4 at Ser111 is not required for channel gating. GLIA 61: 1101-1112
2012
27. Lene L. Rosenbaek, Mette Assentoft, Nis B. Pedersen, Nanna MacAulay & Robert A. Fenton (2012) Characterization of a novel phosphorylations site in the sodium chloride transporter, NCC. Journal of Physiology 590:6121-6139
26. Nanna MacAulay & Thomas Zeuthen (2012) Glial K+ clearance and cell swelling: key roles for cotransporters and pumps. Neurochemical Research 37: 2299-2309
25. Thomas Zeuthen & Nanna MacAulay (2012) Transport of water against its concentration gradient: fact or fiction? WIREs Membrane Transport Signaling 1: 373-381 (doi:10.1002/wmts:54)
24. Thomas Zeuthen & Nanna MacAulay (2012) Cotransport of water by the Na+-K+-2Cl- cotransporter expressed in Xenopus oocytes: NKCC1 versus NKCC2. Journal of Physiology 590: 1139-1154
23. Rikke Søgaard, Ivana Novak & Nanna MacAulay (2012) Differential effect of ammonia on three glutamate transporter isoforms expressed in Xenopus oocytes. American Journal of Physiology – Cell Physiology, 302:880-891
2010
22. Robert A. Fenton, Hanne B. Moeller, Marina Zelenina, Marteinn T. Snaebjornsson, Torgeir Holen & Nanna MacAulay (2010) Differential water permeability and regulation of three AQP4 isoforms. Cellular and Molecular Life Sciences. 67:829-840
21. Nanna MacAulay & Thomas Zeuthen (2010) Water transport between CNS compartments: contribution of aquaporins versus co-transporters. Neuroscience 168:941-956
2009
20. Hanne B. Moeller, Robert A. Fenton, Thomas Zeuthen & Nanna MacAulay (2009) Vasopressin-dependent short-term regulation of AQP4 expressed in Xenopus oocytes. Neuroscience 164:1674-1684
19. Nanna MacAulay, Steffen Hamann & Thomas Zeuthen (2009) Chloride transporters as water pumps: Elements in a new model of epithelial water transport. In: Physiology and pathology of chloride transporters and channels in the nervous system. Eds FJ Alvarez-Leefmans & E Delpire. Academic Press, Elsevier pp 547-568
18. Rikke Soe, Nanna MacAulay & Dan Klaerke (2009) Modulation of Kir4.1 and Kir4.1-Kir5.1 channels by small changes in cell volume. Neurosci. Lett. 457, 80-84
17. Anne-Kristine Meinild, Don D. Loo, Søren Skovstrup, Ulrik Gether & Nanna MacAulay (2009) Elucidating conformational changes in the gamma-aminobutyric acid (GABA)-transporter-1. J. Biol. Chem. 284:16226-15235
16. Rikke Søgaard, Magnus Alsterfjord, Nanna MacAulay & Thomas Zeuthen (2009) Ammonium ion transport by the AMT/Rh homolog TaAMT1;1 is stimulated by acidic pH. Pflugers Arch., 458: 733-743
15. Hanne B Moeller, Nanna MacAulay (shared first author), Mark A Knepper & Robert A Fenton (2009) Role of multiple phosphorylation sites in the carboxyl-terminal tail of aquaporin-2 for water transport: Evidence against channel gating. Am. J. Physiol. Renal Physiol.296, F649-657
2007
14. Thomas Zeuthen, Emil Zeuthen & Nanna MacAulay (2007) Water transport by GLUT2 expressed in Xenopus laevis oocytes. J. Physiol. 579, 345-361
2005
13. Aidas Alaburda, Raul Russo, Nanna MacAulay & Jørn Hounsgaard (2005) Periodic High and Low Conductance States in Spinal Motoneurons and Interneurons during Scratch-like Network Activity in Adult Turtles. J. Neuroscience 25, 6316-21
2004
12. Nanna MacAulay, Steffen Hamann & Thomas Zeuthen (2004) Water Transport in the Brain: Role of Cotransporters. Neuroscience 129, 1031-1044
2003
11. Nanna MacAulay, Anne-Kristine Meinild, Thomas Zeuthen & Ulrik Gether (2003) Residues in the Extracellular Loop 4 are Critical for Maintaining the Conformational Equilibrium of the g-Aminobutyric Acid (GABA) Transporter-1. J. Biol. Chem 278, 28771-28777
10. Morten Grunnet, Thomas Jespersen, Nanna MacAulay, Nanna K. Jørgensen, Nicole Schmitt, Olaf Pongs, Søren-Peter Olesen & Dan Klærke (2003) KCNQ1 Channels Sense Small Changes in Cell Volume. J. Physiol. 549, 419-427
2002
9. Nanna MacAulay, Thomas Zeuthen & Ulrik Gether (2002) Conformational Basis for the Li+-Induced Leak Current in the rat g-Aminobutyric Acid (GABA) Transporter-1. J. Physiol. 544, 447-458
8. Nanna MacAulay, Ulrik Gether, Dan Klærke & Thomas Zeuthen (2002) Passive Water and Urea Permeability of a Human Na+/Glutamate Cotransporter expressed in Xenopus oocytes. J. Physiol. 542, 817-828
7. Morten Grunnet, Nanna MacAulay, Nanna K. Jørgensen, Bo S. Jensen, Søren-Peter Olesen & Dan Klærke (2002) Regulation of Cloned Ca2+-activated K+ Channels by Cell Volume Changes. Pflugers Arch. 444, 167-177
6. Thomas Zeuthen & Nanna MacAulay (2002) Passive Water Transport in Biological Pores. In International Review of Cytology, vol 215, eds T. Zeuthen & W.D. Stein., pp 259-284. Academic Press, San Diego.
5. Thomas Zeuthen & Nanna MacAulay (2002) Cotransporters as Molecular Water Pumps. In International Review of Cytology, vol 215, eds T. Zeuthen & W.D. Stein., pp 203-230. Academic Press, San Diego.
2001
4. Nanna MacAulay, Annie Bendahan, Claus Juul Loland, Thomas Zeuthen, Baruch I.Kanner & Ulrik Gether (2001) Engineered Zn2+ Switches in the GABA Transporter-1: Differential Effects on GABA Uptake and Currents. J. Biol. Chem. 276, 40476-40485
3. Nanna MacAulay, Ulrik Gether, Dan Klærke & Thomas Zeuthen (2001) Water Transport by the Na+-coupled Glutamate Cotransporter Expressed in Xenopus Oocytes. J.Physiol. 530, 367-378
1998
2. Rhoda Blostein, Stewart E. Daly, Nanna Boxenbaum, Lois K. Lane, José M. Arguello, Jerry B. Lingrel, Steve J. D. Karlish & Michael J. Caplan (1998) Conformational Alterations Resulting from Mutations in Cytoplasmic Domains of the Alpha Subunit of the Na,K-ATPase. Acta Physiol. Scand. (Suppl.) 643, 275-281
1. Nanna Boxenbaum, Stewart E. Daly, Zahid Z. Javaid, Lois K. Lane & Rhoda Blostein (1998) Changes in Steady-state Conformational Equilibrium Resulting from Cytoplasmic Mutations of the Na,K-ATPase α-Subunit. J. Biol. Chem. 273, 23086-23092
Lab members
| Name | Title | Job responsibilities | Image |
|---|---|---|---|
| Search in Name | Search in Title | Search in Job responsibilities | |
| Annette Buur Steffensen | Assistant Professor | MacAulay lab |
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| Beatriche Louise Edelbo | PhD Fellow | MacAulay Lab |
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| Dennis Bo Jensen | Postdoc | MacAulay Lab |
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| Jonathan Henry Wardman | Assistant Professor | MacAulay Lab |
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| Nanna MacAulay | Professor | MacAulay Lab |
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| Sara Diana Lolansen | Research Assistant | MacAulay Lab |
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| Søren Norge Andreassen | Postdoc | MacAulay lab |
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| Thomas Zeuthen | Professor Emeritus | MacAulay Lab |
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| Trine Lind Devantier | Laboratory Coordinator | MacAulay Lab |
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| Trine Lisberg Toft | Assistant Professor | MacAulay lab |
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