siRNA-Mediated Downregulation of Gene Expression in Cultured Rat Pineal Cells

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

siRNA-Mediated Downregulation of Gene Expression in Cultured Rat Pineal Cells. / Blancas-Velazquez, Aurea Susana; Rath, Martin Fredensborg.

Methods in Molecular Biology. Humana Press, 2022. p. 113-121 (Methods in Molecular Biology, Vol. 2550).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Blancas-Velazquez, AS & Rath, MF 2022, siRNA-Mediated Downregulation of Gene Expression in Cultured Rat Pineal Cells. in Methods in Molecular Biology. Humana Press, Methods in Molecular Biology, vol. 2550, pp. 113-121. https://doi.org/10.1007/978-1-0716-2593-4_15

APA

Blancas-Velazquez, A. S., & Rath, M. F. (2022). siRNA-Mediated Downregulation of Gene Expression in Cultured Rat Pineal Cells. In Methods in Molecular Biology (pp. 113-121). Humana Press. Methods in Molecular Biology Vol. 2550 https://doi.org/10.1007/978-1-0716-2593-4_15

Vancouver

Blancas-Velazquez AS, Rath MF. siRNA-Mediated Downregulation of Gene Expression in Cultured Rat Pineal Cells. In Methods in Molecular Biology. Humana Press. 2022. p. 113-121. (Methods in Molecular Biology, Vol. 2550). https://doi.org/10.1007/978-1-0716-2593-4_15

Author

Blancas-Velazquez, Aurea Susana ; Rath, Martin Fredensborg. / siRNA-Mediated Downregulation of Gene Expression in Cultured Rat Pineal Cells. Methods in Molecular Biology. Humana Press, 2022. pp. 113-121 (Methods in Molecular Biology, Vol. 2550).

Bibtex

@inbook{a225c0191deb4bc1a733ab925e8d9cce,
title = "siRNA-Mediated Downregulation of Gene Expression in Cultured Rat Pineal Cells",
abstract = "Suspension primary cultures of rat pineal cells have been used for decades to determine biochemical regulatory mechanisms of pineal melatonin synthesis, but more recently, RNA interference technology has made the study of the role of specific genes in this melatonin-proficient model system possible. We here present a protocol for preparing rat pineal cell cultures and efficiently knock down gene expression by use of synthetic siRNA.",
keywords = "Pineal gland, Pinealocyte, Primary cell culture, Rat, RNAi, siRNA",
author = "Blancas-Velazquez, {Aurea Susana} and Rath, {Martin Fredensborg}",
note = "Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2593-4_15",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "113--121",
booktitle = "Methods in Molecular Biology",
address = "United States",

}

RIS

TY - CHAP

T1 - siRNA-Mediated Downregulation of Gene Expression in Cultured Rat Pineal Cells

AU - Blancas-Velazquez, Aurea Susana

AU - Rath, Martin Fredensborg

N1 - Publisher Copyright: © 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

PY - 2022

Y1 - 2022

N2 - Suspension primary cultures of rat pineal cells have been used for decades to determine biochemical regulatory mechanisms of pineal melatonin synthesis, but more recently, RNA interference technology has made the study of the role of specific genes in this melatonin-proficient model system possible. We here present a protocol for preparing rat pineal cell cultures and efficiently knock down gene expression by use of synthetic siRNA.

AB - Suspension primary cultures of rat pineal cells have been used for decades to determine biochemical regulatory mechanisms of pineal melatonin synthesis, but more recently, RNA interference technology has made the study of the role of specific genes in this melatonin-proficient model system possible. We here present a protocol for preparing rat pineal cell cultures and efficiently knock down gene expression by use of synthetic siRNA.

KW - Pineal gland

KW - Pinealocyte

KW - Primary cell culture

KW - Rat

KW - RNAi

KW - siRNA

U2 - 10.1007/978-1-0716-2593-4_15

DO - 10.1007/978-1-0716-2593-4_15

M3 - Book chapter

C2 - 36180684

AN - SCOPUS:85139482336

T3 - Methods in Molecular Biology

SP - 113

EP - 121

BT - Methods in Molecular Biology

PB - Humana Press

ER -

ID: 323006157