siRNA-Mediated Downregulation of Gene Expression in Cultured Rat Pineal Cells
Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
Suspension primary cultures of rat pineal cells have been used for decades to determine biochemical regulatory mechanisms of pineal melatonin synthesis, but more recently, RNA interference technology has made the study of the role of specific genes in this melatonin-proficient model system possible. We here present a protocol for preparing rat pineal cell cultures and efficiently knock down gene expression by use of synthetic siRNA.
Original language | English |
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Title of host publication | Methods in Molecular Biology |
Number of pages | 9 |
Publisher | Humana Press |
Publication date | 2022 |
Pages | 113-121 |
DOIs | |
Publication status | Published - 2022 |
Series | Methods in Molecular Biology |
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Volume | 2550 |
ISSN | 1064-3745 |
Bibliographical note
Publisher Copyright:
© 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
- Pineal gland, Pinealocyte, Primary cell culture, Rat, RNAi, siRNA
Research areas
ID: 323006157