Radiochemical In Situ Hybridization in Developmental Studies of the Pineal Gland
Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
Radiochemical in situ hybridization enables detection of gene expression in small areas of the brain, such as the developing pineal gland in rodents. The method combines determination of spatial and temporal gene expression profiles with semiquantitative analyses. We here describe the procedure of radiochemical in situ hybridization on the developing rat pineal gland ranging from preparation of fetal tissue for in situ hybridization to principles of quantification.
|Title of host publication||Methods in Molecular Biology|
|Number of pages||10|
|Publication status||Published - 2022|
|Series||Methods in Molecular Biology|
© 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
- Brain development, In situ hybridization, Pineal gland, Radioactive, Rodent