Radiochemical In Situ Hybridization in Developmental Studies of the Pineal Gland

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

Radiochemical in situ hybridization enables detection of gene expression in small areas of the brain, such as the developing pineal gland in rodents. The method combines determination of spatial and temporal gene expression profiles with semiquantitative analyses. We here describe the procedure of radiochemical in situ hybridization on the developing rat pineal gland ranging from preparation of fetal tissue for in situ hybridization to principles of quantification.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Number of pages10
PublisherHumana Press
Publication date2022
Publication statusPublished - 2022
SeriesMethods in Molecular Biology

Bibliographical note

Publisher Copyright:
© 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

    Research areas

  • Brain development, In situ hybridization, Pineal gland, Radioactive, Rodent

ID: 323006087