Radiochemical In Situ Hybridization in Developmental Studies of the Pineal Gland
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Radiochemical In Situ Hybridization in Developmental Studies of the Pineal Gland. / Rath, Martin Fredensborg; Møller, Morten.
Methods in Molecular Biology. Humana Press, 2022. p. 75-84 (Methods in Molecular Biology, Vol. 2550).Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
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TY - CHAP
T1 - Radiochemical In Situ Hybridization in Developmental Studies of the Pineal Gland
AU - Rath, Martin Fredensborg
AU - Møller, Morten
N1 - Publisher Copyright: © 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2022
Y1 - 2022
N2 - Radiochemical in situ hybridization enables detection of gene expression in small areas of the brain, such as the developing pineal gland in rodents. The method combines determination of spatial and temporal gene expression profiles with semiquantitative analyses. We here describe the procedure of radiochemical in situ hybridization on the developing rat pineal gland ranging from preparation of fetal tissue for in situ hybridization to principles of quantification.
AB - Radiochemical in situ hybridization enables detection of gene expression in small areas of the brain, such as the developing pineal gland in rodents. The method combines determination of spatial and temporal gene expression profiles with semiquantitative analyses. We here describe the procedure of radiochemical in situ hybridization on the developing rat pineal gland ranging from preparation of fetal tissue for in situ hybridization to principles of quantification.
KW - Brain development
KW - In situ hybridization
KW - Pineal gland
KW - Radioactive
KW - Rodent
U2 - 10.1007/978-1-0716-2593-4_10
DO - 10.1007/978-1-0716-2593-4_10
M3 - Book chapter
C2 - 36180679
AN - SCOPUS:85139490557
T3 - Methods in Molecular Biology
SP - 75
EP - 84
BT - Methods in Molecular Biology
PB - Humana Press
ER -
ID: 323006087