Measurements of Exocytosis by Capacitance Recordings and Calcium Uncaging in Mouse Adrenal Chromaffin Cells
Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
Fusion of vesicles with the plasma membrane and liberation of their contents is a multistep process involving several proteins. Correctly assigning the role of specific proteins and reactions in this cascade requires a measurement method with high temporal resolution. Patch-clamp recordings of cell membrane capacitance in combination with calcium measurements, calcium uncaging, and carbon-fiber amperometry allow for the accurate determination of vesicle pool sizes, their fusion kinetics, and their secreted oxidizable content. Here, we will describe this method in a model system for neurosecretion, the adrenal chromaffin cells, which secrete adrenaline.
Original language | English |
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Title of host publication | Methods in Molecular Biology |
Editors | Florence Niedergang, Nicolas Vitale, Stéphane Gasman |
Number of pages | 19 |
Place of Publication | New Yorj |
Publisher | Humana Press |
Publication date | 2021 |
Edition | 1 |
Pages | 233-251 |
ISBN (Print) | 978-1-0716-1043-5 |
ISBN (Electronic) | 978-1-0716-1044-2 |
DOIs | |
Publication status | Published - 2021 |
Series | Methods in Molecular Biology |
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Volume | 2233 |
ISSN | 1064-3745 |
- Calcium measurements, Capacitance measurement, Carbon-fiber amperometry, Chromaffin cells, Electrophysiology, Exocytosis, Neurosecretion, Patch-clamp, Vesicles
Research areas
ID: 258774298