Measurements of Exocytosis by Capacitance Recordings and Calcium Uncaging in Mouse Adrenal Chromaffin Cells

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

Measurements of Exocytosis by Capacitance Recordings and Calcium Uncaging in Mouse Adrenal Chromaffin Cells. / Houy, Sébastien; Martins, Joana S.; Mohrmann, Ralf; Sørensen, Jakob Balslev.

Methods in Molecular Biology. ed. / Florence Niedergang; Nicolas Vitale; Stéphane Gasman. 1. ed. New Yorj : Humana Press, 2021. p. 233-251 (Methods in Molecular Biology, Vol. 2233).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Houy, S, Martins, JS, Mohrmann, R & Sørensen, JB 2021, Measurements of Exocytosis by Capacitance Recordings and Calcium Uncaging in Mouse Adrenal Chromaffin Cells. in F Niedergang, N Vitale & S Gasman (eds), Methods in Molecular Biology. 1 edn, Humana Press, New Yorj, Methods in Molecular Biology, vol. 2233, pp. 233-251. https://doi.org/10.1007/978-1-0716-1044-2_16

APA

Houy, S., Martins, J. S., Mohrmann, R., & Sørensen, J. B. (2021). Measurements of Exocytosis by Capacitance Recordings and Calcium Uncaging in Mouse Adrenal Chromaffin Cells. In F. Niedergang, N. Vitale, & S. Gasman (Eds.), Methods in Molecular Biology (1 ed., pp. 233-251). Humana Press. Methods in Molecular Biology Vol. 2233 https://doi.org/10.1007/978-1-0716-1044-2_16

Vancouver

Houy S, Martins JS, Mohrmann R, Sørensen JB. Measurements of Exocytosis by Capacitance Recordings and Calcium Uncaging in Mouse Adrenal Chromaffin Cells. In Niedergang F, Vitale N, Gasman S, editors, Methods in Molecular Biology. 1 ed. New Yorj: Humana Press. 2021. p. 233-251. (Methods in Molecular Biology, Vol. 2233). https://doi.org/10.1007/978-1-0716-1044-2_16

Author

Houy, Sébastien ; Martins, Joana S. ; Mohrmann, Ralf ; Sørensen, Jakob Balslev. / Measurements of Exocytosis by Capacitance Recordings and Calcium Uncaging in Mouse Adrenal Chromaffin Cells. Methods in Molecular Biology. editor / Florence Niedergang ; Nicolas Vitale ; Stéphane Gasman. 1. ed. New Yorj : Humana Press, 2021. pp. 233-251 (Methods in Molecular Biology, Vol. 2233).

Bibtex

@inbook{9108b2c16bad44349303ba195899a0db,
title = "Measurements of Exocytosis by Capacitance Recordings and Calcium Uncaging in Mouse Adrenal Chromaffin Cells",
abstract = "Fusion of vesicles with the plasma membrane and liberation of their contents is a multistep process involving several proteins. Correctly assigning the role of specific proteins and reactions in this cascade requires a measurement method with high temporal resolution. Patch-clamp recordings of cell membrane capacitance in combination with calcium measurements, calcium uncaging, and carbon-fiber amperometry allow for the accurate determination of vesicle pool sizes, their fusion kinetics, and their secreted oxidizable content. Here, we will describe this method in a model system for neurosecretion, the adrenal chromaffin cells, which secrete adrenaline.",
keywords = "Calcium measurements, Capacitance measurement, Carbon-fiber amperometry, Chromaffin cells, Electrophysiology, Exocytosis, Neurosecretion, Patch-clamp, Vesicles",
author = "S{\'e}bastien Houy and Martins, {Joana S.} and Ralf Mohrmann and S{\o}rensen, {Jakob Balslev}",
year = "2021",
doi = "10.1007/978-1-0716-1044-2_16",
language = "English",
isbn = "978-1-0716-1043-5",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "233--251",
editor = "Niedergang, {Florence } and Vitale, {Nicolas } and Gasman, {St{\'e}phane }",
booktitle = "Methods in Molecular Biology",
address = "United States",
edition = "1",

}

RIS

TY - CHAP

T1 - Measurements of Exocytosis by Capacitance Recordings and Calcium Uncaging in Mouse Adrenal Chromaffin Cells

AU - Houy, Sébastien

AU - Martins, Joana S.

AU - Mohrmann, Ralf

AU - Sørensen, Jakob Balslev

PY - 2021

Y1 - 2021

N2 - Fusion of vesicles with the plasma membrane and liberation of their contents is a multistep process involving several proteins. Correctly assigning the role of specific proteins and reactions in this cascade requires a measurement method with high temporal resolution. Patch-clamp recordings of cell membrane capacitance in combination with calcium measurements, calcium uncaging, and carbon-fiber amperometry allow for the accurate determination of vesicle pool sizes, their fusion kinetics, and their secreted oxidizable content. Here, we will describe this method in a model system for neurosecretion, the adrenal chromaffin cells, which secrete adrenaline.

AB - Fusion of vesicles with the plasma membrane and liberation of their contents is a multistep process involving several proteins. Correctly assigning the role of specific proteins and reactions in this cascade requires a measurement method with high temporal resolution. Patch-clamp recordings of cell membrane capacitance in combination with calcium measurements, calcium uncaging, and carbon-fiber amperometry allow for the accurate determination of vesicle pool sizes, their fusion kinetics, and their secreted oxidizable content. Here, we will describe this method in a model system for neurosecretion, the adrenal chromaffin cells, which secrete adrenaline.

KW - Calcium measurements

KW - Capacitance measurement

KW - Carbon-fiber amperometry

KW - Chromaffin cells

KW - Electrophysiology

KW - Exocytosis

KW - Neurosecretion

KW - Patch-clamp

KW - Vesicles

U2 - 10.1007/978-1-0716-1044-2_16

DO - 10.1007/978-1-0716-1044-2_16

M3 - Book chapter

C2 - 33222139

AN - SCOPUS:85096529660

SN - 978-1-0716-1043-5

T3 - Methods in Molecular Biology

SP - 233

EP - 251

BT - Methods in Molecular Biology

A2 - Niedergang, Florence

A2 - Vitale, Nicolas

A2 - Gasman, Stéphane

PB - Humana Press

CY - New Yorj

ER -

ID: 258774298