TY - JOUR

T1 - In-vitro Recordings of Neural Magnetic Activity From the Auditory Brainstem Using Color Centers in Diamond

T2 - A Simulation Study

AU - Karadas, Mursel

AU - Olsson, Christoffer

AU - Winther Hansen, Nikolaj

AU - Perrier, Jean-Francois

AU - Webb, James Luke

AU - Huck, Alexander

AU - Andersen, Ulrik Lund

AU - Thielscher, Axel

PY - 2021

Y1 - 2021

N2 - Magnetometry based on nitrogen-vacancy (NV) centers in diamond is a novel technique capable of measuring magnetic fields with high sensitivity and high spatial resolution. With the further advancements of these sensors, they may open up novel approaches for the 2D imaging of neural signals in vitro. In the present study, we investigate the feasibility of NV-based imaging by numerically simulating the magnetic signal from the auditory pathway of a rodent brainstem slice (ventral cochlear nucleus, VCN, to the medial trapezoid body, MNTB) as stimulated by both electric and optic stimulation. The resulting signal from these two stimulation methods are evaluated and compared. A realistic pathway model was created based on published data of the neural morphologies and channel dynamics of the globular bushy cells in the VCN and their axonal projections to the principal cells in the MNTB. The pathway dynamics in response to optic and electric stimulation and the emitted magnetic fields were estimated using the cable equation. For simulating the optic stimulation, the light distribution in brain tissue was numerically estimated and used to model the optogenetic neural excitation based on a four state channelrhodopsin-2 (ChR2) model. The corresponding heating was also estimated, using the bio-heat equation and was found to be low (

AB - Magnetometry based on nitrogen-vacancy (NV) centers in diamond is a novel technique capable of measuring magnetic fields with high sensitivity and high spatial resolution. With the further advancements of these sensors, they may open up novel approaches for the 2D imaging of neural signals in vitro. In the present study, we investigate the feasibility of NV-based imaging by numerically simulating the magnetic signal from the auditory pathway of a rodent brainstem slice (ventral cochlear nucleus, VCN, to the medial trapezoid body, MNTB) as stimulated by both electric and optic stimulation. The resulting signal from these two stimulation methods are evaluated and compared. A realistic pathway model was created based on published data of the neural morphologies and channel dynamics of the globular bushy cells in the VCN and their axonal projections to the principal cells in the MNTB. The pathway dynamics in response to optic and electric stimulation and the emitted magnetic fields were estimated using the cable equation. For simulating the optic stimulation, the light distribution in brain tissue was numerically estimated and used to model the optogenetic neural excitation based on a four state channelrhodopsin-2 (ChR2) model. The corresponding heating was also estimated, using the bio-heat equation and was found to be low (

KW - cable equation

KW - neural magnetic field

KW - magnetometry

KW - NV centers

KW - Monte Carlo

KW - optogenetics

KW - fiber optics

KW - Kubelka-Munk model

KW - UNDERLYING OPTICAL STIMULATION

KW - POTASSIUM CHANNEL

KW - COCHLEAR NUCLEUS

KW - MEDIAL NUCLEUS

KW - TRAPEZOID BODY

KW - MOUSE CALYX

KW - KINETICS

KW - SYNAPSE

KW - LIGHT

KW - FIELD

U2 - 10.3389/fnins.2021.643614

DO - 10.3389/fnins.2021.643614

M3 - Journal article

C2 - 34054404

VL - 15

JO - Frontiers in Neuroscience

JF - Frontiers in Neuroscience

SN - 1662-4548

M1 - 643614

ER -