Double-label and conventional deoxyglucose methods: a practical guide for the user.

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The autoradiographic deoxyglucose method is widely used to map functional activity in mammalian brain. Whereas the method is simple to use, the underlying kinetic model is complex. This paper reviews the deoxyglucose kinetic model and the relevant implications for the user who does not have extensive knowledge of tracer kinetics. In generally understandable terms, single-label and double-label deoxyglucose approaches are discussed. Experimental procedures are described in detail. The calculations required for qualitative and quantitative experiments are explained. The deoxyglucose method is compared to other methods that map functional activity in mammalian brain.
Original languageEnglish
JournalCerebrovascular and Brain Metabolism Reviews
Volume1
Issue number4
Pages (from-to)319-67
Number of pages48
ISSN1040-8827
Publication statusPublished - 1989
Externally publishedYes

ID: 14943159