A single dose of cocaine raises SV2A density in hippocampus of adolescent rats

Research output: Contribution to journalJournal articlepeer-review

  • Rachele Rossi
  • Simone Larsen Bærentzen
  • Majken B. Thomsen
  • Caroline C. Real
  • Gregers Wegener
  • Rodrigo Grassi-Oliveira
  • Gjedde, Albert
  • Anne M. Landau

Objective: Cocaine is a highly addictive psychostimulant that affects synaptic activity with structural and functional adaptations of neurons. The transmembrane synaptic vesicle glycoprotein 2A (SV2A) of pre-synaptic vesicles is commonly used to measure synaptic density, as a novel approach to the detection of synaptic changes. We do not know if a single dose of cocaine suffices to affect pre-synaptic SV2A density, especially during adolescence when synapses undergo intense maturation. Here, we explored potential changes of pre-synaptic SV2A density in target brain areas associated with the cocaine-induced boost of dopaminergic neurotransmission, specifically testing if the effects would last after the return of dopamine levels to baseline. Methods: We administered cocaine (20 mg/kg i.p.) or saline to rats in early adolescence, tested their activity levels, and removed the brains 1 hour and 7 days after injection. To evaluate immediate and lasting effects, we did autoradiography with [3H]UCB-J, a specific tracer for SV2A, in medial prefrontal cortex, striatum, nucleus accumbens, amygdala, and dorsal and ventral areas of hippocampus. We also measured the striatal binding of [3H]GBR-12935 to test cocaine's occupancy of the dopamine transporter at both times of study. Results: We found a significant increase of [3H]UCB-J binding in the dorsal and ventral sections of hippocampus 7 days after the cocaine administration compared to saline-injected rats, but no differences 1 hour after the injection. The [3H]GBR-12935 binding remained unchanged at both times. Conclusion: Cocaine provoked lasting changes of hippocampal synaptic density after a single exposure during adolescence.

Original languageEnglish
JournalActa Neuropsychiatrica
Publication statusE-pub ahead of print - 2023

Bibliographical note

Publisher Copyright:
© 2023 Cambridge University Press. All rights reserved.

    Research areas

  • adolescent, autoradiography, cocaine, neuronal plasticity, rats

ID: 340114950