A single dose of cocaine raises SV2A density in hippocampus of adolescent rats
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A single dose of cocaine raises SV2A density in hippocampus of adolescent rats. / Rossi, Rachele; Bærentzen, Simone Larsen; Thomsen, Majken B.; Real, Caroline C.; Wegener, Gregers; Grassi-Oliveira, Rodrigo; Gjedde, Albert; Landau, Anne M.
In: Acta Neuropsychiatrica, Vol. 36, No. 2, 2024, p. 109-117.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - A single dose of cocaine raises SV2A density in hippocampus of adolescent rats
AU - Rossi, Rachele
AU - Bærentzen, Simone Larsen
AU - Thomsen, Majken B.
AU - Real, Caroline C.
AU - Wegener, Gregers
AU - Grassi-Oliveira, Rodrigo
AU - Gjedde, Albert
AU - Landau, Anne M.
N1 - Publisher Copyright: © 2023 Cambridge University Press. All rights reserved.
PY - 2024
Y1 - 2024
N2 - Objective: Cocaine is a highly addictive psychostimulant that affects synaptic activity with structural and functional adaptations of neurons. The transmembrane synaptic vesicle glycoprotein 2A (SV2A) of pre-synaptic vesicles is commonly used to measure synaptic density, as a novel approach to the detection of synaptic changes. We do not know if a single dose of cocaine suffices to affect pre-synaptic SV2A density, especially during adolescence when synapses undergo intense maturation. Here, we explored potential changes of pre-synaptic SV2A density in target brain areas associated with the cocaine-induced boost of dopaminergic neurotransmission, specifically testing if the effects would last after the return of dopamine levels to baseline. Methods: We administered cocaine (20 mg/kg i.p.) or saline to rats in early adolescence, tested their activity levels, and removed the brains 1 hour and 7 days after injection. To evaluate immediate and lasting effects, we did autoradiography with [3H]UCB-J, a specific tracer for SV2A, in medial prefrontal cortex, striatum, nucleus accumbens, amygdala, and dorsal and ventral areas of hippocampus. We also measured the striatal binding of [3H]GBR-12935 to test cocaine's occupancy of the dopamine transporter at both times of study. Results: We found a significant increase of [3H]UCB-J binding in the dorsal and ventral sections of hippocampus 7 days after the cocaine administration compared to saline-injected rats, but no differences 1 hour after the injection. The [3H]GBR-12935 binding remained unchanged at both times. Conclusion: Cocaine provoked lasting changes of hippocampal synaptic density after a single exposure during adolescence.
AB - Objective: Cocaine is a highly addictive psychostimulant that affects synaptic activity with structural and functional adaptations of neurons. The transmembrane synaptic vesicle glycoprotein 2A (SV2A) of pre-synaptic vesicles is commonly used to measure synaptic density, as a novel approach to the detection of synaptic changes. We do not know if a single dose of cocaine suffices to affect pre-synaptic SV2A density, especially during adolescence when synapses undergo intense maturation. Here, we explored potential changes of pre-synaptic SV2A density in target brain areas associated with the cocaine-induced boost of dopaminergic neurotransmission, specifically testing if the effects would last after the return of dopamine levels to baseline. Methods: We administered cocaine (20 mg/kg i.p.) or saline to rats in early adolescence, tested their activity levels, and removed the brains 1 hour and 7 days after injection. To evaluate immediate and lasting effects, we did autoradiography with [3H]UCB-J, a specific tracer for SV2A, in medial prefrontal cortex, striatum, nucleus accumbens, amygdala, and dorsal and ventral areas of hippocampus. We also measured the striatal binding of [3H]GBR-12935 to test cocaine's occupancy of the dopamine transporter at both times of study. Results: We found a significant increase of [3H]UCB-J binding in the dorsal and ventral sections of hippocampus 7 days after the cocaine administration compared to saline-injected rats, but no differences 1 hour after the injection. The [3H]GBR-12935 binding remained unchanged at both times. Conclusion: Cocaine provoked lasting changes of hippocampal synaptic density after a single exposure during adolescence.
KW - adolescent
KW - autoradiography
KW - cocaine
KW - neuronal plasticity
KW - rats
U2 - 10.1017/neu.2023.14
DO - 10.1017/neu.2023.14
M3 - Journal article
C2 - 36847240
AN - SCOPUS:85149945229
VL - 36
SP - 109
EP - 117
JO - Acta Neuropsychiatrica
JF - Acta Neuropsychiatrica
SN - 0924-2708
IS - 2
ER -
ID: 340114950