3D Analysis of Synaptic Ultrastructure in Organotypic Hippocampal Slice Culture by High-Pressure Freezing and Electron Tomography

Research output: Contribution to journal › Journal article › Research

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3D Analysis of Synaptic Ultrastructure in Organotypic Hippocampal Slice Culture by High-Pressure Freezing and Electron Tomography. / Imig, Cordelia; Cooper, Benjamin H.

In: Methods in molecular biology (Clifton, N.J.), Vol. 1538, 2017, p. 215-231.

Research output: Contribution to journal › Journal article › Research

Harvard

Imig, C & Cooper, BH 2017, '3D Analysis of Synaptic Ultrastructure in Organotypic Hippocampal Slice Culture by High-Pressure Freezing and Electron Tomography', Methods in molecular biology (Clifton, N.J.), vol. 1538, pp. 215-231. https://doi.org/10.1007/978-1-4939-6688-2_15

APA

Imig, C., & Cooper, B. H. (2017). 3D Analysis of Synaptic Ultrastructure in Organotypic Hippocampal Slice Culture by High-Pressure Freezing and Electron Tomography. Methods in molecular biology (Clifton, N.J.), 1538, 215-231. https://doi.org/10.1007/978-1-4939-6688-2_15

Vancouver

Imig C, Cooper BH. 3D Analysis of Synaptic Ultrastructure in Organotypic Hippocampal Slice Culture by High-Pressure Freezing and Electron Tomography. Methods in molecular biology (Clifton, N.J.). 2017;1538:215-231. https://doi.org/10.1007/978-1-4939-6688-2_15

Author

Imig, Cordelia ; Cooper, Benjamin H. / 3D Analysis of Synaptic Ultrastructure in Organotypic Hippocampal Slice Culture by High-Pressure Freezing and Electron Tomography. In: Methods in molecular biology (Clifton, N.J.). 2017 ; Vol. 1538. pp. 215-231.

Bibtex

@article{400e8a78038a462db4c08c1932ac2d7f,
title = "3D Analysis of Synaptic Ultrastructure in Organotypic Hippocampal Slice Culture by High-Pressure Freezing and Electron Tomography",
abstract = "Transmission electron microscopy serves as a valuable tool for synaptic structure-function analyses aimed at identifying morphological features or modifications associated with specific developmental stages or dysfunctional synaptic states. By utilizing cryo-preparation techniques to minimize the introduction of structural artifacts during sample preparation, and electron tomography to reconstruct the 3D ultrastructural architecture of a synapse, the spatial organization and morphological properties of synaptic organelles and subcompartments can be quantified with unparalleled precision. In this chapter, we present an experimental approach combining organotypic slice culture, high-pressure freezing, automated freeze-substitution, and electron tomography to investigate spatial relationships between synaptic vesicles and active zone release sites in synapses from lethal mouse mutants.",
keywords = "Animals, Electron Microscope Tomography/methods, Hippocampus/cytology, Image Processing, Computer-Assisted, Imaging, Three-Dimensional/methods, Mice, Microscopy, Electron, Transmission/methods, Synapses/ultrastructure",
author = "Cordelia Imig and Cooper, {Benjamin H}",
year = "2017",
doi = "10.1007/978-1-4939-6688-2_15",
language = "English",
volume = "1538",
pages = "215--231",
journal = "Methods in Molecular Biology",
issn = "1064-3745",
publisher = "Humana Press",

}

RIS

TY - JOUR

T1 - 3D Analysis of Synaptic Ultrastructure in Organotypic Hippocampal Slice Culture by High-Pressure Freezing and Electron Tomography

AU - Imig, Cordelia

AU - Cooper, Benjamin H

PY - 2017

Y1 - 2017

N2 - Transmission electron microscopy serves as a valuable tool for synaptic structure-function analyses aimed at identifying morphological features or modifications associated with specific developmental stages or dysfunctional synaptic states. By utilizing cryo-preparation techniques to minimize the introduction of structural artifacts during sample preparation, and electron tomography to reconstruct the 3D ultrastructural architecture of a synapse, the spatial organization and morphological properties of synaptic organelles and subcompartments can be quantified with unparalleled precision. In this chapter, we present an experimental approach combining organotypic slice culture, high-pressure freezing, automated freeze-substitution, and electron tomography to investigate spatial relationships between synaptic vesicles and active zone release sites in synapses from lethal mouse mutants.

AB - Transmission electron microscopy serves as a valuable tool for synaptic structure-function analyses aimed at identifying morphological features or modifications associated with specific developmental stages or dysfunctional synaptic states. By utilizing cryo-preparation techniques to minimize the introduction of structural artifacts during sample preparation, and electron tomography to reconstruct the 3D ultrastructural architecture of a synapse, the spatial organization and morphological properties of synaptic organelles and subcompartments can be quantified with unparalleled precision. In this chapter, we present an experimental approach combining organotypic slice culture, high-pressure freezing, automated freeze-substitution, and electron tomography to investigate spatial relationships between synaptic vesicles and active zone release sites in synapses from lethal mouse mutants.

KW - Animals

KW - Electron Microscope Tomography/methods

KW - Hippocampus/cytology

KW - Image Processing, Computer-Assisted

KW - Imaging, Three-Dimensional/methods

KW - Mice

KW - Microscopy, Electron, Transmission/methods

KW - Synapses/ultrastructure

U2 - 10.1007/978-1-4939-6688-2_15

DO - 10.1007/978-1-4939-6688-2_15

M3 - Journal article

C2 - 27943193

VL - 1538

SP - 215

EP - 231

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

ER -

ID: 237697866