3D Analysis of Synaptic Ultrastructure in Organotypic Hippocampal Slice Culture by High-Pressure Freezing and Electron Tomography
Research output: Contribution to journal › Journal article › Research
Transmission electron microscopy serves as a valuable tool for synaptic structure-function analyses aimed at identifying morphological features or modifications associated with specific developmental stages or dysfunctional synaptic states. By utilizing cryo-preparation techniques to minimize the introduction of structural artifacts during sample preparation, and electron tomography to reconstruct the 3D ultrastructural architecture of a synapse, the spatial organization and morphological properties of synaptic organelles and subcompartments can be quantified with unparalleled precision. In this chapter, we present an experimental approach combining organotypic slice culture, high-pressure freezing, automated freeze-substitution, and electron tomography to investigate spatial relationships between synaptic vesicles and active zone release sites in synapses from lethal mouse mutants.
Original language | English |
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Journal | Methods in molecular biology (Clifton, N.J.) |
Volume | 1538 |
Pages (from-to) | 215-231 |
Number of pages | 17 |
ISSN | 1064-3745 |
DOIs | |
Publication status | Published - 2017 |
Externally published | Yes |
- Animals, Electron Microscope Tomography/methods, Hippocampus/cytology, Image Processing, Computer-Assisted, Imaging, Three-Dimensional/methods, Mice, Microscopy, Electron, Transmission/methods, Synapses/ultrastructure
Research areas
ID: 237697866