Water transport by the Na+/glucose cotransporter under isotonic conditions

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Water transport by the Na+/glucose cotransporter under isotonic conditions. / Zeuthen, T; Meinild, A K; Klaerke, D A; Loo, D D; Wright, E M; Belhage, B; Litman, T.

In: Biology of the Cell, Vol. 89, No. 5-6, 1997, p. 307-12.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Zeuthen, T, Meinild, AK, Klaerke, DA, Loo, DD, Wright, EM, Belhage, B & Litman, T 1997, 'Water transport by the Na+/glucose cotransporter under isotonic conditions', Biology of the Cell, vol. 89, no. 5-6, pp. 307-12.

APA

Zeuthen, T., Meinild, A. K., Klaerke, D. A., Loo, D. D., Wright, E. M., Belhage, B., & Litman, T. (1997). Water transport by the Na+/glucose cotransporter under isotonic conditions. Biology of the Cell, 89(5-6), 307-12.

Vancouver

Zeuthen T, Meinild AK, Klaerke DA, Loo DD, Wright EM, Belhage B et al. Water transport by the Na+/glucose cotransporter under isotonic conditions. Biology of the Cell. 1997;89(5-6):307-12.

Author

Zeuthen, T ; Meinild, A K ; Klaerke, D A ; Loo, D D ; Wright, E M ; Belhage, B ; Litman, T. / Water transport by the Na+/glucose cotransporter under isotonic conditions. In: Biology of the Cell. 1997 ; Vol. 89, No. 5-6. pp. 307-12.

Bibtex

@article{bae4d8d0f1a511dcbee902004c4f4f50,
title = "Water transport by the Na+/glucose cotransporter under isotonic conditions",
abstract = "Solute cotransport in the Na+/glucose cotransporter is directly coupled to significant water fluxes. The water fluxes are energized by the downhill fluxes of the other substrates by a mechanism within the protein itself. In the present paper we investigate the Na+/glucose cotransporter expressed in Xenopus oocytes. We present a method which allows short-term exposures to sugar under voltage clamp conditions. We demonstrate that water is cotransported with the solutes despite no osmotic differences between the external and intracellular solutions. There is a fixed ratio of 195:1 between the number of water molecules and the number of Na+ ions transported, equivalent to 390 water molecules per glucose molecule. Unstirred layer effects are ruled out on the basis of experiments on native oocytes incubated with the ionophores gramicidin D or nystatin.",
author = "T Zeuthen and Meinild, {A K} and Klaerke, {D A} and Loo, {D D} and Wright, {E M} and B Belhage and T Litman",
note = "Keywords: Animals; Anti-Bacterial Agents; Biological Transport; Electrophysiology; Female; Glucose; Gramicidin; Ionophores; Isotonic Solutions; Membrane Glycoproteins; Monosaccharide Transport Proteins; Nystatin; Oocytes; Sodium; Sodium-Glucose Transporter 1; Water; Xenopus laevis",
year = "1997",
language = "English",
volume = "89",
pages = "307--12",
journal = "Biology of the Cell",
issn = "0248-4900",
publisher = "Portland Press Ltd.",
number = "5-6",

}

RIS

TY - JOUR

T1 - Water transport by the Na+/glucose cotransporter under isotonic conditions

AU - Zeuthen, T

AU - Meinild, A K

AU - Klaerke, D A

AU - Loo, D D

AU - Wright, E M

AU - Belhage, B

AU - Litman, T

N1 - Keywords: Animals; Anti-Bacterial Agents; Biological Transport; Electrophysiology; Female; Glucose; Gramicidin; Ionophores; Isotonic Solutions; Membrane Glycoproteins; Monosaccharide Transport Proteins; Nystatin; Oocytes; Sodium; Sodium-Glucose Transporter 1; Water; Xenopus laevis

PY - 1997

Y1 - 1997

N2 - Solute cotransport in the Na+/glucose cotransporter is directly coupled to significant water fluxes. The water fluxes are energized by the downhill fluxes of the other substrates by a mechanism within the protein itself. In the present paper we investigate the Na+/glucose cotransporter expressed in Xenopus oocytes. We present a method which allows short-term exposures to sugar under voltage clamp conditions. We demonstrate that water is cotransported with the solutes despite no osmotic differences between the external and intracellular solutions. There is a fixed ratio of 195:1 between the number of water molecules and the number of Na+ ions transported, equivalent to 390 water molecules per glucose molecule. Unstirred layer effects are ruled out on the basis of experiments on native oocytes incubated with the ionophores gramicidin D or nystatin.

AB - Solute cotransport in the Na+/glucose cotransporter is directly coupled to significant water fluxes. The water fluxes are energized by the downhill fluxes of the other substrates by a mechanism within the protein itself. In the present paper we investigate the Na+/glucose cotransporter expressed in Xenopus oocytes. We present a method which allows short-term exposures to sugar under voltage clamp conditions. We demonstrate that water is cotransported with the solutes despite no osmotic differences between the external and intracellular solutions. There is a fixed ratio of 195:1 between the number of water molecules and the number of Na+ ions transported, equivalent to 390 water molecules per glucose molecule. Unstirred layer effects are ruled out on the basis of experiments on native oocytes incubated with the ionophores gramicidin D or nystatin.

M3 - Journal article

C2 - 9468601

VL - 89

SP - 307

EP - 312

JO - Biology of the Cell

JF - Biology of the Cell

SN - 0248-4900

IS - 5-6

ER -

ID: 3153763