Transcranial photoacoustic imaging of NMDA-evoked focal circuit dynamics in the rat hippocampus

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Transcranial photoacoustic imaging of NMDA-evoked focal circuit dynamics in the rat hippocampus. / Kang, Jeeun; Kadam, Shilpa D.; Elmore, Joshua S.; Sullivan, Brennan J.; Valentine, Heather; Malla, Adarsha P.; Harraz, Maged M.; Rahmim, Arman; Kang, Jin U.; Loew, Leslie M.; Baumann, Michael H.; Grace, Anthony A.; Gjedde, Albert; Boctor, Emad M.; Wong, Dean F.

In: Journal of Neural Engineering, Vol. 17, No. 2, 025001, 2020.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Kang, J, Kadam, SD, Elmore, JS, Sullivan, BJ, Valentine, H, Malla, AP, Harraz, MM, Rahmim, A, Kang, JU, Loew, LM, Baumann, MH, Grace, AA, Gjedde, A, Boctor, EM & Wong, DF 2020, 'Transcranial photoacoustic imaging of NMDA-evoked focal circuit dynamics in the rat hippocampus', Journal of Neural Engineering, vol. 17, no. 2, 025001. https://doi.org/10.1088/1741-2552/ab78ca

APA

Kang, J., Kadam, S. D., Elmore, J. S., Sullivan, B. J., Valentine, H., Malla, A. P., Harraz, M. M., Rahmim, A., Kang, J. U., Loew, L. M., Baumann, M. H., Grace, A. A., Gjedde, A., Boctor, E. M., & Wong, D. F. (2020). Transcranial photoacoustic imaging of NMDA-evoked focal circuit dynamics in the rat hippocampus. Journal of Neural Engineering, 17(2), [025001]. https://doi.org/10.1088/1741-2552/ab78ca

Vancouver

Kang J, Kadam SD, Elmore JS, Sullivan BJ, Valentine H, Malla AP et al. Transcranial photoacoustic imaging of NMDA-evoked focal circuit dynamics in the rat hippocampus. Journal of Neural Engineering. 2020;17(2). 025001. https://doi.org/10.1088/1741-2552/ab78ca

Author

Kang, Jeeun ; Kadam, Shilpa D. ; Elmore, Joshua S. ; Sullivan, Brennan J. ; Valentine, Heather ; Malla, Adarsha P. ; Harraz, Maged M. ; Rahmim, Arman ; Kang, Jin U. ; Loew, Leslie M. ; Baumann, Michael H. ; Grace, Anthony A. ; Gjedde, Albert ; Boctor, Emad M. ; Wong, Dean F. / Transcranial photoacoustic imaging of NMDA-evoked focal circuit dynamics in the rat hippocampus. In: Journal of Neural Engineering. 2020 ; Vol. 17, No. 2.

Bibtex

@article{77b7710549f141a2b33ac5d4a10fa8cf,
title = "Transcranial photoacoustic imaging of NMDA-evoked focal circuit dynamics in the rat hippocampus",
abstract = "Objective. We report the transcranial functional photoacoustic (fPA) neuroimaging of N-methyl-D-aspartate (NMDA) evoked neural activity in the rat hippocampus. Concurrent quantitative electroencephalography (qEEG) and microdialysis were used to record real-time circuit dynamics and excitatory neurotransmitter concentrations, respectively.Approach. We hypothesized that location-specific fPA voltage-sensitive dye (VSD) contrast would identify neural activity changes in the hippocampus which correlate with NMDA-evoked excitatory neurotransmission.Main results. Transcranial fPA VSD imaging at the contralateral side of the microdialysis probe provided NMDA-evoked VSD responses with positive correlation to extracellular glutamate concentration changes. qEEG validated a wide range of glutamatergic excitation, which culminated in focal seizure activity after a high NMDA dose. We conclude that transcranial fPA VSD imaging can distinguish focal glutamate loads in the rat hippocampus, based on the VSD redistribution mechanism which is sensitive to the electrophysiologic membrane potential.Significance. Our results suggest the future utility of this emerging technology in both laboratory and clinical sciences as an innovative functional neuroimaging modality.",
keywords = "photoacoustic, voltage-sensitive dye, microdialysis, quantitative electroencephalogram, N-methyl-D-aspartate, glutamate, hippocampus, IN-VIVO, SPATIOTEMPORAL DYNAMICS, TOMOGRAPHY, ULTRASOUND, MICRODIALYSIS, MODEL, RESOLUTION, METHYLONE, SENSOR, FRAMES",
author = "Jeeun Kang and Kadam, {Shilpa D.} and Elmore, {Joshua S.} and Sullivan, {Brennan J.} and Heather Valentine and Malla, {Adarsha P.} and Harraz, {Maged M.} and Arman Rahmim and Kang, {Jin U.} and Loew, {Leslie M.} and Baumann, {Michael H.} and Grace, {Anthony A.} and Albert Gjedde and Boctor, {Emad M.} and Wong, {Dean F.}",
year = "2020",
doi = "10.1088/1741-2552/ab78ca",
language = "English",
volume = "17",
journal = "Journal of Neural Engineering",
issn = "1741-2560",
publisher = "Institute of Physics Publishing Ltd",
number = "2",

}

RIS

TY - JOUR

T1 - Transcranial photoacoustic imaging of NMDA-evoked focal circuit dynamics in the rat hippocampus

AU - Kang, Jeeun

AU - Kadam, Shilpa D.

AU - Elmore, Joshua S.

AU - Sullivan, Brennan J.

AU - Valentine, Heather

AU - Malla, Adarsha P.

AU - Harraz, Maged M.

AU - Rahmim, Arman

AU - Kang, Jin U.

AU - Loew, Leslie M.

AU - Baumann, Michael H.

AU - Grace, Anthony A.

AU - Gjedde, Albert

AU - Boctor, Emad M.

AU - Wong, Dean F.

PY - 2020

Y1 - 2020

N2 - Objective. We report the transcranial functional photoacoustic (fPA) neuroimaging of N-methyl-D-aspartate (NMDA) evoked neural activity in the rat hippocampus. Concurrent quantitative electroencephalography (qEEG) and microdialysis were used to record real-time circuit dynamics and excitatory neurotransmitter concentrations, respectively.Approach. We hypothesized that location-specific fPA voltage-sensitive dye (VSD) contrast would identify neural activity changes in the hippocampus which correlate with NMDA-evoked excitatory neurotransmission.Main results. Transcranial fPA VSD imaging at the contralateral side of the microdialysis probe provided NMDA-evoked VSD responses with positive correlation to extracellular glutamate concentration changes. qEEG validated a wide range of glutamatergic excitation, which culminated in focal seizure activity after a high NMDA dose. We conclude that transcranial fPA VSD imaging can distinguish focal glutamate loads in the rat hippocampus, based on the VSD redistribution mechanism which is sensitive to the electrophysiologic membrane potential.Significance. Our results suggest the future utility of this emerging technology in both laboratory and clinical sciences as an innovative functional neuroimaging modality.

AB - Objective. We report the transcranial functional photoacoustic (fPA) neuroimaging of N-methyl-D-aspartate (NMDA) evoked neural activity in the rat hippocampus. Concurrent quantitative electroencephalography (qEEG) and microdialysis were used to record real-time circuit dynamics and excitatory neurotransmitter concentrations, respectively.Approach. We hypothesized that location-specific fPA voltage-sensitive dye (VSD) contrast would identify neural activity changes in the hippocampus which correlate with NMDA-evoked excitatory neurotransmission.Main results. Transcranial fPA VSD imaging at the contralateral side of the microdialysis probe provided NMDA-evoked VSD responses with positive correlation to extracellular glutamate concentration changes. qEEG validated a wide range of glutamatergic excitation, which culminated in focal seizure activity after a high NMDA dose. We conclude that transcranial fPA VSD imaging can distinguish focal glutamate loads in the rat hippocampus, based on the VSD redistribution mechanism which is sensitive to the electrophysiologic membrane potential.Significance. Our results suggest the future utility of this emerging technology in both laboratory and clinical sciences as an innovative functional neuroimaging modality.

KW - photoacoustic

KW - voltage-sensitive dye

KW - microdialysis

KW - quantitative electroencephalogram

KW - N-methyl-D-aspartate

KW - glutamate

KW - hippocampus

KW - IN-VIVO

KW - SPATIOTEMPORAL DYNAMICS

KW - TOMOGRAPHY

KW - ULTRASOUND

KW - MICRODIALYSIS

KW - MODEL

KW - RESOLUTION

KW - METHYLONE

KW - SENSOR

KW - FRAMES

U2 - 10.1088/1741-2552/ab78ca

DO - 10.1088/1741-2552/ab78ca

M3 - Journal article

C2 - 32084654

VL - 17

JO - Journal of Neural Engineering

JF - Journal of Neural Engineering

SN - 1741-2560

IS - 2

M1 - 025001

ER -

ID: 248334526