The modulatory effect of substance P on rat pineal norepinephrine release and melatonin secretion
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The modulatory effect of substance P on rat pineal norepinephrine release and melatonin secretion. / Mukda, Sujira; Møller, Morten; Ebadi, Manuchair; Govitrapong, Piyarat; Møller, Morten.
In: Neuroscience Letters, Vol. 461, No. 3, 2009, p. 258-61.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - The modulatory effect of substance P on rat pineal norepinephrine release and melatonin secretion
AU - Mukda, Sujira
AU - Møller, Morten
AU - Ebadi, Manuchair
AU - Govitrapong, Piyarat
AU - Møller, Morten
PY - 2009
Y1 - 2009
N2 - Secretion of melatonin by the mammalian pineal gland is primarily regulated by the release of norepinephrine (NE) from sympathetic nerve terminals that originate from the superior cervical ganglia. Peptidergic nerves that originate in the perikarya located in the sensory trigeminal ganglia also innervate the pineal gland. Some of these peptidergic nerve fibers contain substance P. Previously, we have characterized neurokinin 1 type substance P receptors in the pineal gland. However, the function of this receptor in the pineal gland remains unclear. Here, we examined the modulatory effect of substance P on rat pineal NE transmission. We show that at the presynaptic level, substance P stimulates the KCl-induced [(3)H]NE release from the pineal nerve ending. However, we found that substance P did not affect the basal levels of either arylalkylamine-N-acetyltransferase (AANAT) activity or melatonin secretion in rat pineal organ cultures. However, in the presence of NE, substance P inhibited the NE-induced increase in AANAT activity and melatonin secretion. This is the first time that a function for substance P in the mammalian pineal gland has been demonstrated.
AB - Secretion of melatonin by the mammalian pineal gland is primarily regulated by the release of norepinephrine (NE) from sympathetic nerve terminals that originate from the superior cervical ganglia. Peptidergic nerves that originate in the perikarya located in the sensory trigeminal ganglia also innervate the pineal gland. Some of these peptidergic nerve fibers contain substance P. Previously, we have characterized neurokinin 1 type substance P receptors in the pineal gland. However, the function of this receptor in the pineal gland remains unclear. Here, we examined the modulatory effect of substance P on rat pineal NE transmission. We show that at the presynaptic level, substance P stimulates the KCl-induced [(3)H]NE release from the pineal nerve ending. However, we found that substance P did not affect the basal levels of either arylalkylamine-N-acetyltransferase (AANAT) activity or melatonin secretion in rat pineal organ cultures. However, in the presence of NE, substance P inhibited the NE-induced increase in AANAT activity and melatonin secretion. This is the first time that a function for substance P in the mammalian pineal gland has been demonstrated.
KW - Animals
KW - Arylalkylamine N-Acetyltransferase
KW - Cells, Cultured
KW - Male
KW - Melatonin
KW - Neurotransmitter Agents
KW - Norepinephrine
KW - Pineal Gland
KW - Potassium Chloride
KW - Rats
KW - Rats, Wistar
KW - Receptors, Neurokinin-1
KW - Substance P
KW - Synapses
U2 - 10.1016/j.neulet.2009.06.040
DO - 10.1016/j.neulet.2009.06.040
M3 - Journal article
C2 - 19545608
VL - 461
SP - 258
EP - 261
JO - Neuroscience letters. Supplement
JF - Neuroscience letters. Supplement
SN - 0167-6253
IS - 3
ER -
ID: 38059917