Subclasses of histamine H3 antagonist binding sites in rat brain.

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Subclasses of histamine H3 antagonist binding sites in rat brain. / Cumming, P; Gjedde, A.

In: Brain Research, Vol. 641, No. 2, 1994, p. 203-7.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Cumming, P & Gjedde, A 1994, 'Subclasses of histamine H3 antagonist binding sites in rat brain.', Brain Research, vol. 641, no. 2, pp. 203-7.

APA

Cumming, P., & Gjedde, A. (1994). Subclasses of histamine H3 antagonist binding sites in rat brain. Brain Research, 641(2), 203-7.

Vancouver

Cumming P, Gjedde A. Subclasses of histamine H3 antagonist binding sites in rat brain. Brain Research. 1994;641(2):203-7.

Author

Cumming, P ; Gjedde, A. / Subclasses of histamine H3 antagonist binding sites in rat brain. In: Brain Research. 1994 ; Vol. 641, No. 2. pp. 203-7.

Bibtex

@article{15c1c880b31511debc73000ea68e967b,
title = "Subclasses of histamine H3 antagonist binding sites in rat brain.",
abstract = "Histamine H3 antagonists have been reported to discriminate subclasses of histamine H3 agonist binding in rat cortical membranes. This phenomenon was investigated by autoradiography of cryostat sections of rat forebrain labelled with [3H]N alpha-methylhistamine ([3H]NAMH, 4 nM). Displacement curves with thioperamide detected a single site in cortex and striatum (pIC50 = 8.18 +/- 0.03). However, Hill coefficients (nH = 0.51 +/- 0.12) suggested the possible presence of multiple binding sites. Displacement with burimamide was consistent with two site models in all brain regions examined (pIC50(A) = 7.9 +/- 1.5; pIC50(B) = 5.6 +/- 0.7), except for the medial septum where a single site was detected. Elsewhere, the relative abundance of the two sites displaced by burimamide (H3A:H3B) appeared to be 1:2. Thioperamide may have failed to discriminate two sites because the IC50s were too similar to be distinguished in the present autoradiographic study.",
author = "P Cumming and A Gjedde",
year = "1994",
language = "English",
volume = "641",
pages = "203--7",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "2",

}

RIS

TY - JOUR

T1 - Subclasses of histamine H3 antagonist binding sites in rat brain.

AU - Cumming, P

AU - Gjedde, A

PY - 1994

Y1 - 1994

N2 - Histamine H3 antagonists have been reported to discriminate subclasses of histamine H3 agonist binding in rat cortical membranes. This phenomenon was investigated by autoradiography of cryostat sections of rat forebrain labelled with [3H]N alpha-methylhistamine ([3H]NAMH, 4 nM). Displacement curves with thioperamide detected a single site in cortex and striatum (pIC50 = 8.18 +/- 0.03). However, Hill coefficients (nH = 0.51 +/- 0.12) suggested the possible presence of multiple binding sites. Displacement with burimamide was consistent with two site models in all brain regions examined (pIC50(A) = 7.9 +/- 1.5; pIC50(B) = 5.6 +/- 0.7), except for the medial septum where a single site was detected. Elsewhere, the relative abundance of the two sites displaced by burimamide (H3A:H3B) appeared to be 1:2. Thioperamide may have failed to discriminate two sites because the IC50s were too similar to be distinguished in the present autoradiographic study.

AB - Histamine H3 antagonists have been reported to discriminate subclasses of histamine H3 agonist binding in rat cortical membranes. This phenomenon was investigated by autoradiography of cryostat sections of rat forebrain labelled with [3H]N alpha-methylhistamine ([3H]NAMH, 4 nM). Displacement curves with thioperamide detected a single site in cortex and striatum (pIC50 = 8.18 +/- 0.03). However, Hill coefficients (nH = 0.51 +/- 0.12) suggested the possible presence of multiple binding sites. Displacement with burimamide was consistent with two site models in all brain regions examined (pIC50(A) = 7.9 +/- 1.5; pIC50(B) = 5.6 +/- 0.7), except for the medial septum where a single site was detected. Elsewhere, the relative abundance of the two sites displaced by burimamide (H3A:H3B) appeared to be 1:2. Thioperamide may have failed to discriminate two sites because the IC50s were too similar to be distinguished in the present autoradiographic study.

M3 - Journal article

C2 - 8012822

VL - 641

SP - 203

EP - 207

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 2

ER -

ID: 14944391