Spontaneous calcium waves in granule cells in cerebellar slice cultures

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Spontaneous calcium waves in granule cells in cerebellar slice cultures. / Apuschkin, Mia; Ougaard, Maria; Rekling, Jens C.

In: Neuroscience Letters, Vol. 553, 11.10.2013, p. 78-83.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Apuschkin, M, Ougaard, M & Rekling, JC 2013, 'Spontaneous calcium waves in granule cells in cerebellar slice cultures', Neuroscience Letters, vol. 553, pp. 78-83. https://doi.org/10.1016/j.neulet.2013.08.022

APA

Apuschkin, M., Ougaard, M., & Rekling, J. C. (2013). Spontaneous calcium waves in granule cells in cerebellar slice cultures. Neuroscience Letters, 553, 78-83. https://doi.org/10.1016/j.neulet.2013.08.022

Vancouver

Apuschkin M, Ougaard M, Rekling JC. Spontaneous calcium waves in granule cells in cerebellar slice cultures. Neuroscience Letters. 2013 Oct 11;553:78-83. https://doi.org/10.1016/j.neulet.2013.08.022

Author

Apuschkin, Mia ; Ougaard, Maria ; Rekling, Jens C. / Spontaneous calcium waves in granule cells in cerebellar slice cultures. In: Neuroscience Letters. 2013 ; Vol. 553. pp. 78-83.

Bibtex

@article{0099fc1aafe64173b21cba8a96b756b3,

title = "Spontaneous calcium waves in granule cells in cerebellar slice cultures",

abstract = "Multiple regions in the CNS display propagating correlated activity during embryonic and postnatal development. This activity can be recorded as waves of increased calcium concentrations in spiking neurons or glia cells, and have been suggested to be involved in patterning, axonal guidance and establishment of synaptic transmission. Here, we used calcium imaging in slice cultures of the postnatal cerebellum, and observe spontaneous propagating calcium waves in NeuN-positive granule-like cells. Wave formation was blocked by TTX and the AMPA antagonist NBQX, but persisted after NMDA receptor blockade with MK-801. Whole-cell recordings during wave formation showed cyclic EPSP barrages with an amplitude of 10-20 mV concurrent with wave activity. Local non-propagating putative transglial waves were also present in the cultures, and could be reproduced by pressure application of ATP. We hypothesize, that the propagating wave activity is carried through the tissue by axonal collaterals formed by neighboring granule cells, and further suggest that the correlated activity may be related to processes that ensure correct postnatal wiring of the cerebellar circuits.",

keywords = "Animals, Calcium, Cerebellum, Mice, Neurons, Patch-Clamp Techniques, Receptors, AMPA, Synaptic Transmission",

author = "Mia Apuschkin and Maria Ougaard and Rekling, {Jens C}",

year = "2013",

month = oct,

day = "11",

doi = "10.1016/j.neulet.2013.08.022",

language = "English",

volume = "553",

pages = "78--83",

journal = "Neuroscience letters. Supplement",

issn = "0167-6253",

publisher = "Elsevier Ireland Ltd",

}

RIS

TY - JOUR

T1 - Spontaneous calcium waves in granule cells in cerebellar slice cultures

AU - Apuschkin, Mia

AU - Ougaard, Maria

AU - Rekling, Jens C

PY - 2013/10/11

Y1 - 2013/10/11

N2 - Multiple regions in the CNS display propagating correlated activity during embryonic and postnatal development. This activity can be recorded as waves of increased calcium concentrations in spiking neurons or glia cells, and have been suggested to be involved in patterning, axonal guidance and establishment of synaptic transmission. Here, we used calcium imaging in slice cultures of the postnatal cerebellum, and observe spontaneous propagating calcium waves in NeuN-positive granule-like cells. Wave formation was blocked by TTX and the AMPA antagonist NBQX, but persisted after NMDA receptor blockade with MK-801. Whole-cell recordings during wave formation showed cyclic EPSP barrages with an amplitude of 10-20 mV concurrent with wave activity. Local non-propagating putative transglial waves were also present in the cultures, and could be reproduced by pressure application of ATP. We hypothesize, that the propagating wave activity is carried through the tissue by axonal collaterals formed by neighboring granule cells, and further suggest that the correlated activity may be related to processes that ensure correct postnatal wiring of the cerebellar circuits.

AB - Multiple regions in the CNS display propagating correlated activity during embryonic and postnatal development. This activity can be recorded as waves of increased calcium concentrations in spiking neurons or glia cells, and have been suggested to be involved in patterning, axonal guidance and establishment of synaptic transmission. Here, we used calcium imaging in slice cultures of the postnatal cerebellum, and observe spontaneous propagating calcium waves in NeuN-positive granule-like cells. Wave formation was blocked by TTX and the AMPA antagonist NBQX, but persisted after NMDA receptor blockade with MK-801. Whole-cell recordings during wave formation showed cyclic EPSP barrages with an amplitude of 10-20 mV concurrent with wave activity. Local non-propagating putative transglial waves were also present in the cultures, and could be reproduced by pressure application of ATP. We hypothesize, that the propagating wave activity is carried through the tissue by axonal collaterals formed by neighboring granule cells, and further suggest that the correlated activity may be related to processes that ensure correct postnatal wiring of the cerebellar circuits.

KW - Animals

KW - Calcium

KW - Cerebellum

KW - Mice

KW - Neurons

KW - Patch-Clamp Techniques

KW - Receptors, AMPA

KW - Synaptic Transmission

U2 - 10.1016/j.neulet.2013.08.022

DO - 10.1016/j.neulet.2013.08.022

M3 - Journal article

C2 - 23973304

VL - 553

SP - 78

EP - 83

JO - Neuroscience letters. Supplement

JF - Neuroscience letters. Supplement

SN - 0167-6253

ER -

ID: 120073999

Department of Neuroscience