Sacral Spinal Cord Transection and Isolated Sacral Cord Preparation to Study Chronic Spinal Cord Injury in Adult Mice

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Sacral Spinal Cord Transection and Isolated Sacral Cord Preparation to Study Chronic Spinal Cord Injury in Adult Mice. / Bellardita, Carmelo; Marcantoni, Maite; Löw, Peter; Kiehn, Ole.

In: Bio-protocol, Vol. 8, No. 7, e2784, 2018.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Bellardita, C, Marcantoni, M, Löw, P & Kiehn, O 2018, 'Sacral Spinal Cord Transection and Isolated Sacral Cord Preparation to Study Chronic Spinal Cord Injury in Adult Mice', Bio-protocol, vol. 8, no. 7, e2784. https://doi.org/10.21769/BioProtoc.2784

APA

Bellardita, C., Marcantoni, M., Löw, P., & Kiehn, O. (2018). Sacral Spinal Cord Transection and Isolated Sacral Cord Preparation to Study Chronic Spinal Cord Injury in Adult Mice. Bio-protocol, 8(7), [e2784]. https://doi.org/10.21769/BioProtoc.2784

Vancouver

Bellardita C, Marcantoni M, Löw P, Kiehn O. Sacral Spinal Cord Transection and Isolated Sacral Cord Preparation to Study Chronic Spinal Cord Injury in Adult Mice. Bio-protocol. 2018;8(7). e2784. https://doi.org/10.21769/BioProtoc.2784

Author

Bellardita, Carmelo ; Marcantoni, Maite ; Löw, Peter ; Kiehn, Ole. / Sacral Spinal Cord Transection and Isolated Sacral Cord Preparation to Study Chronic Spinal Cord Injury in Adult Mice. In: Bio-protocol. 2018 ; Vol. 8, No. 7.

Bibtex

@article{6cc37e1cf3d046e3a080d012a9bcd167,
title = "Sacral Spinal Cord Transection and Isolated Sacral Cord Preparation to Study Chronic Spinal Cord Injury in Adult Mice",
abstract = "Spinal cord injury (SCI) is characterized by multiple sensory/motor impairments that arise from different underlying neural mechanisms. Linking specific sensory/motor impairments to neural mechanism is limited by a lack of direct experimental access to these neural circuits. Here, we describe an experimental model which addresses this shortcoming. We generated a mouse model of chronic spinal cord injury that reliably reproduces spasticity observed after SCI, while at the same time allows study of motor impairments in vivo and in an in vitro preparation of the spinal cord. The model allows for the combination of mouse genetics in in vitro and in vivo conditions with advanced imaging, behavioral analysis, and detailed electrophysiology, techniques which are not easily applied in conventional SCI models.",
author = "Carmelo Bellardita and Maite Marcantoni and Peter L{\"o}w and Ole Kiehn",
year = "2018",
doi = "10.21769/BioProtoc.2784",
language = "English",
volume = "8",
journal = "Bio-protocol",
issn = "2331-8325",
publisher = "bio-protocol",
number = "7",

}

RIS

TY - JOUR

T1 - Sacral Spinal Cord Transection and Isolated Sacral Cord Preparation to Study Chronic Spinal Cord Injury in Adult Mice

AU - Bellardita, Carmelo

AU - Marcantoni, Maite

AU - Löw, Peter

AU - Kiehn, Ole

PY - 2018

Y1 - 2018

N2 - Spinal cord injury (SCI) is characterized by multiple sensory/motor impairments that arise from different underlying neural mechanisms. Linking specific sensory/motor impairments to neural mechanism is limited by a lack of direct experimental access to these neural circuits. Here, we describe an experimental model which addresses this shortcoming. We generated a mouse model of chronic spinal cord injury that reliably reproduces spasticity observed after SCI, while at the same time allows study of motor impairments in vivo and in an in vitro preparation of the spinal cord. The model allows for the combination of mouse genetics in in vitro and in vivo conditions with advanced imaging, behavioral analysis, and detailed electrophysiology, techniques which are not easily applied in conventional SCI models.

AB - Spinal cord injury (SCI) is characterized by multiple sensory/motor impairments that arise from different underlying neural mechanisms. Linking specific sensory/motor impairments to neural mechanism is limited by a lack of direct experimental access to these neural circuits. Here, we describe an experimental model which addresses this shortcoming. We generated a mouse model of chronic spinal cord injury that reliably reproduces spasticity observed after SCI, while at the same time allows study of motor impairments in vivo and in an in vitro preparation of the spinal cord. The model allows for the combination of mouse genetics in in vitro and in vivo conditions with advanced imaging, behavioral analysis, and detailed electrophysiology, techniques which are not easily applied in conventional SCI models.

U2 - 10.21769/BioProtoc.2784

DO - 10.21769/BioProtoc.2784

M3 - Journal article

C2 - 29795778

VL - 8

JO - Bio-protocol

JF - Bio-protocol

SN - 2331-8325

IS - 7

M1 - e2784

ER -

ID: 202478327