Robust CNS regeneration after complete spinal cord transection using aligned poly-L-lactic acid microfibers
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Robust CNS regeneration after complete spinal cord transection using aligned poly-L-lactic acid microfibers. / Hurtado, Andres; Cregg, Jared M; Wang, Han B; Wendell, Dane F; Oudega, Martin; Gilbert, Ryan J; McDonald, John W.
In: Biomaterials, Vol. 32, No. 26, 09.2011, p. 6068-79.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Robust CNS regeneration after complete spinal cord transection using aligned poly-L-lactic acid microfibers
AU - Hurtado, Andres
AU - Cregg, Jared M
AU - Wang, Han B
AU - Wendell, Dane F
AU - Oudega, Martin
AU - Gilbert, Ryan J
AU - McDonald, John W
N1 - Copyright © 2011 Elsevier Ltd. All rights reserved.
PY - 2011/9
Y1 - 2011/9
N2 - Following spinal cord injury, axons fail to regenerate without exogenous intervention. In this study we report that aligned microfiber-based grafts foster robust regeneration of vascularized CNS tissue. Film, random, and aligned microfiber-based conduits were grafted into a 3 mm thoracic rat spinal cord gap created by complete transection. Over the course of 4 weeks, microtopography presented by aligned or random poly-L-lactic acid microfibers facilitated infiltration of host tissue, and the initial 3 mm gap was closed by endogenous cell populations. This bulk tissue response was composed of regenerating axons accompanied by morphologically aligned astrocytes. Aligned fibers promoted long distance (2055 ± 150 μm), rostrocaudal axonal regeneration, significantly greater than random fiber (1162 ± 87 μm) and film (413 ± 199 μm) controls. Retrograde tracing indicated that regenerating axons originated from propriospinal neurons of the rostral spinal cord, and supraspinal neurons of the reticular formation, red nucleus, raphe and vestibular nuclei. Our findings outline a form of regeneration within the central nervous system that holds important implications for regeneration biology.
AB - Following spinal cord injury, axons fail to regenerate without exogenous intervention. In this study we report that aligned microfiber-based grafts foster robust regeneration of vascularized CNS tissue. Film, random, and aligned microfiber-based conduits were grafted into a 3 mm thoracic rat spinal cord gap created by complete transection. Over the course of 4 weeks, microtopography presented by aligned or random poly-L-lactic acid microfibers facilitated infiltration of host tissue, and the initial 3 mm gap was closed by endogenous cell populations. This bulk tissue response was composed of regenerating axons accompanied by morphologically aligned astrocytes. Aligned fibers promoted long distance (2055 ± 150 μm), rostrocaudal axonal regeneration, significantly greater than random fiber (1162 ± 87 μm) and film (413 ± 199 μm) controls. Retrograde tracing indicated that regenerating axons originated from propriospinal neurons of the rostral spinal cord, and supraspinal neurons of the reticular formation, red nucleus, raphe and vestibular nuclei. Our findings outline a form of regeneration within the central nervous system that holds important implications for regeneration biology.
KW - Animals
KW - Astrocytes/cytology
KW - Cells, Cultured
KW - Central Nervous System/physiology
KW - Female
KW - Ganglia, Spinal/cytology
KW - Immunohistochemistry
KW - Lactic Acid/chemistry
KW - Neurites/metabolism
KW - Polyesters
KW - Polymers/chemistry
KW - Rats
KW - Rats, Sprague-Dawley
KW - Spinal Cord Injuries/therapy
KW - X-Ray Microtomography
U2 - 10.1016/j.biomaterials.2011.05.006
DO - 10.1016/j.biomaterials.2011.05.006
M3 - Journal article
C2 - 21636129
VL - 32
SP - 6068
EP - 6079
JO - Biomaterials
JF - Biomaterials
SN - 0142-9612
IS - 26
ER -
ID: 248114429