TY - JOUR

T1 - Rapid simultaneous determination of regional blood flow and blood-brain glucose transfer in brain of rat.

AU - Gjedde, A

AU - Hansen, A J

AU - Siemkowicz, E

PY - 1980

Y1 - 1980

N2 - A new method was developed and used in rat to measure regional and whole-brain blood flow and blood-brain glucose transfer simultaneously and in 20 s. This simple method consisted of i.v. bolus injection of labeled butanol and tracer glucose, determination of the average arterial tracer concentration and subsequent assay of cerebral tissue activity 20 s after bolus injection. The whole-brain blood flow rate averaged 129 ml (100 g)-1 min-1. The unidirectional blood-brain glucose transfer was twice as high as previously estimated in similar studies on rat, or 144 mumol (100 g)-1 min-1 at 10 mM glucose in plasma. The magnitude is sufficient to explain the high cerebral glucose consumption rates recently determined by means of autoradiographic 2-deoxy-D-glucose method of Sokoloff et al. (1977).

AB - A new method was developed and used in rat to measure regional and whole-brain blood flow and blood-brain glucose transfer simultaneously and in 20 s. This simple method consisted of i.v. bolus injection of labeled butanol and tracer glucose, determination of the average arterial tracer concentration and subsequent assay of cerebral tissue activity 20 s after bolus injection. The whole-brain blood flow rate averaged 129 ml (100 g)-1 min-1. The unidirectional blood-brain glucose transfer was twice as high as previously estimated in similar studies on rat, or 144 mumol (100 g)-1 min-1 at 10 mM glucose in plasma. The magnitude is sufficient to explain the high cerebral glucose consumption rates recently determined by means of autoradiographic 2-deoxy-D-glucose method of Sokoloff et al. (1977).

M3 - Journal article

C2 - 7415845

VL - 108

SP - 321

EP - 330

JO - Acta Physiologica Scandinavica

JF - Acta Physiologica Scandinavica

SN - 0001-6772

IS - 4

ER -