Rapid functional genetics of the oligodendrocyte lineage using pluripotent stem cells

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Rapid functional genetics of the oligodendrocyte lineage using pluripotent stem cells. / Lager, Angela M; Corradin, Olivia G; Cregg, Jared M; Elitt, Matthew S; Shick, H Elizabeth; Clayton, Benjamin L L; Allan, Kevin C; Olsen, Hannah E; Madhavan, Mayur; Tesar, Paul J.

In: Nature Communications, Vol. 9, No. 1, 3708, 13.09.2018.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Lager, AM, Corradin, OG, Cregg, JM, Elitt, MS, Shick, HE, Clayton, BLL, Allan, KC, Olsen, HE, Madhavan, M & Tesar, PJ 2018, 'Rapid functional genetics of the oligodendrocyte lineage using pluripotent stem cells', Nature Communications, vol. 9, no. 1, 3708. https://doi.org/10.1038/s41467-018-06102-7

APA

Lager, A. M., Corradin, O. G., Cregg, J. M., Elitt, M. S., Shick, H. E., Clayton, B. L. L., Allan, K. C., Olsen, H. E., Madhavan, M., & Tesar, P. J. (2018). Rapid functional genetics of the oligodendrocyte lineage using pluripotent stem cells. Nature Communications, 9(1), [3708]. https://doi.org/10.1038/s41467-018-06102-7

Vancouver

Lager AM, Corradin OG, Cregg JM, Elitt MS, Shick HE, Clayton BLL et al. Rapid functional genetics of the oligodendrocyte lineage using pluripotent stem cells. Nature Communications. 2018 Sep 13;9(1). 3708. https://doi.org/10.1038/s41467-018-06102-7

Author

Lager, Angela M ; Corradin, Olivia G ; Cregg, Jared M ; Elitt, Matthew S ; Shick, H Elizabeth ; Clayton, Benjamin L L ; Allan, Kevin C ; Olsen, Hannah E ; Madhavan, Mayur ; Tesar, Paul J. / Rapid functional genetics of the oligodendrocyte lineage using pluripotent stem cells. In: Nature Communications. 2018 ; Vol. 9, No. 1.

Bibtex

@article{a53a0b28588f4bb6a630da51966ea539,
title = "Rapid functional genetics of the oligodendrocyte lineage using pluripotent stem cells",
abstract = "Oligodendrocyte dysfunction underlies many neurological disorders, but rapid assessment of mutation-specific effects in these cells has been impractical. To enable functional genetics in oligodendrocytes, here we report a highly efficient method for generating oligodendrocytes and their progenitors from mouse embryonic and induced pluripotent stem cells, independent of mouse strain or mutational status. We demonstrate that this approach, when combined with genome engineering, provides a powerful platform for the expeditious study of genotype-phenotype relationships in oligodendrocytes.",
keywords = "Alleles, Animals, CRISPR-Cas Systems, Cell Differentiation/genetics, Cell Lineage, DNA Mutational Analysis, Genetic Association Studies, Genetic Engineering, Genotype, Induced Pluripotent Stem Cells, Lentivirus, Male, Mice, Mice, Inbred C57BL, Mice, Knockout, Neurons/metabolism, Oligodendroglia/cytology, Pluripotent Stem Cells/cytology",
author = "Lager, {Angela M} and Corradin, {Olivia G} and Cregg, {Jared M} and Elitt, {Matthew S} and Shick, {H Elizabeth} and Clayton, {Benjamin L L} and Allan, {Kevin C} and Olsen, {Hannah E} and Mayur Madhavan and Tesar, {Paul J}",
year = "2018",
month = sep,
day = "13",
doi = "10.1038/s41467-018-06102-7",
language = "English",
volume = "9",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "nature publishing group",
number = "1",

}

RIS

TY - JOUR

T1 - Rapid functional genetics of the oligodendrocyte lineage using pluripotent stem cells

AU - Lager, Angela M

AU - Corradin, Olivia G

AU - Cregg, Jared M

AU - Elitt, Matthew S

AU - Shick, H Elizabeth

AU - Clayton, Benjamin L L

AU - Allan, Kevin C

AU - Olsen, Hannah E

AU - Madhavan, Mayur

AU - Tesar, Paul J

PY - 2018/9/13

Y1 - 2018/9/13

N2 - Oligodendrocyte dysfunction underlies many neurological disorders, but rapid assessment of mutation-specific effects in these cells has been impractical. To enable functional genetics in oligodendrocytes, here we report a highly efficient method for generating oligodendrocytes and their progenitors from mouse embryonic and induced pluripotent stem cells, independent of mouse strain or mutational status. We demonstrate that this approach, when combined with genome engineering, provides a powerful platform for the expeditious study of genotype-phenotype relationships in oligodendrocytes.

AB - Oligodendrocyte dysfunction underlies many neurological disorders, but rapid assessment of mutation-specific effects in these cells has been impractical. To enable functional genetics in oligodendrocytes, here we report a highly efficient method for generating oligodendrocytes and their progenitors from mouse embryonic and induced pluripotent stem cells, independent of mouse strain or mutational status. We demonstrate that this approach, when combined with genome engineering, provides a powerful platform for the expeditious study of genotype-phenotype relationships in oligodendrocytes.

KW - Alleles

KW - Animals

KW - CRISPR-Cas Systems

KW - Cell Differentiation/genetics

KW - Cell Lineage

KW - DNA Mutational Analysis

KW - Genetic Association Studies

KW - Genetic Engineering

KW - Genotype

KW - Induced Pluripotent Stem Cells

KW - Lentivirus

KW - Male

KW - Mice

KW - Mice, Inbred C57BL

KW - Mice, Knockout

KW - Neurons/metabolism

KW - Oligodendroglia/cytology

KW - Pluripotent Stem Cells/cytology

U2 - 10.1038/s41467-018-06102-7

DO - 10.1038/s41467-018-06102-7

M3 - Journal article

C2 - 30213958

VL - 9

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

IS - 1

M1 - 3708

ER -

ID: 248113898