TY - JOUR

T1 - Rapid and long-term induction of effector immediate early genes (BDNF, Neuritin and Arc) in peri-infarct cortex and dentate gyrus after ischemic injury in rat brain

AU - Rickhag, Karl Mattias

AU - Teilum, Maria

AU - Wieloch, Tadeusz

PY - 2007/6/2

Y1 - 2007/6/2

N2 - The genomic response following brain ischemia is very complex and involves activation of both protective and detrimental signaling pathways. Immediate early genes (IEGs) represent the first wave of gene expression following ischemia and are induced in extensive regions of the ischemic brain including cerebral cortex and hippocampus. Brain-derived neurotrophic factor (BDNF), Neuritin and Activity-regulated cytoskeleton-associated protein (Arc) belong to a subgroup of immediate early genes implicated in synaptic plasticity known as effector immediate early genes. Here, we investigated the spatial and temporal activation pattern for these genes during the first 24 h of reperfusion following 2-h occlusion of the middle cerebral artery. Neuritin showed a persistent activation in frontal-cingulate cortex while Arc displayed a biphasic response. Also, in dentate gyrus, activation was observed at 0-6 h of reperfusion for Neuritin and 0-12 h of reperfusion for Arc while BDNF was induced 0-9 h of reperfusion. Our study demonstrates a rapid and long-term activation of effector immediate early genes in distinct brain areas following ischemic injury in rat. Effector gene activation may be part of long-term synaptic responses of ischemic brain tissue.

AB - The genomic response following brain ischemia is very complex and involves activation of both protective and detrimental signaling pathways. Immediate early genes (IEGs) represent the first wave of gene expression following ischemia and are induced in extensive regions of the ischemic brain including cerebral cortex and hippocampus. Brain-derived neurotrophic factor (BDNF), Neuritin and Activity-regulated cytoskeleton-associated protein (Arc) belong to a subgroup of immediate early genes implicated in synaptic plasticity known as effector immediate early genes. Here, we investigated the spatial and temporal activation pattern for these genes during the first 24 h of reperfusion following 2-h occlusion of the middle cerebral artery. Neuritin showed a persistent activation in frontal-cingulate cortex while Arc displayed a biphasic response. Also, in dentate gyrus, activation was observed at 0-6 h of reperfusion for Neuritin and 0-12 h of reperfusion for Arc while BDNF was induced 0-9 h of reperfusion. Our study demonstrates a rapid and long-term activation of effector immediate early genes in distinct brain areas following ischemic injury in rat. Effector gene activation may be part of long-term synaptic responses of ischemic brain tissue.

KW - Animals

KW - Apoptosis Regulatory Proteins

KW - Brain Infarction

KW - Brain Ischemia

KW - Brain-Derived Neurotrophic Factor

KW - Cerebral Cortex

KW - Dentate Gyrus

KW - GPI-Linked Proteins

KW - Gene Expression Regulation

KW - In Situ Hybridization

KW - Male

KW - Muscle Proteins

KW - Neuropeptides

KW - RNA, Messenger

KW - Rats

KW - Rats, Wistar

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Time Factors

KW - Transcriptional Activation

U2 - 10.1016/j.brainres.2007.03.005

DO - 10.1016/j.brainres.2007.03.005

M3 - Journal article

C2 - 17397810

VL - 1151

SP - 203

EP - 210

JO - Brain Research

JF - Brain Research

SN - 0006-8993

ER -