Rab2 drives axonal transport of dense core vesicles and lysosomal organelles

Research output: Contribution to journal › Journal article › Research › peer-review

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Rab2 drives axonal transport of dense core vesicles and lysosomal organelles. / Lund, Viktor Karlovich; Lycas, Matthew Domenic; Schack, Anders; Andersen, Rita Chan; Gether, Ulrik; Kjaerulff, Ole.

In: Cell Reports, Vol. 35, No. 2, 108973, 2021.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Lund, VK, Lycas, MD, Schack, A, Andersen, RC, Gether, U & Kjaerulff, O 2021, 'Rab2 drives axonal transport of dense core vesicles and lysosomal organelles', Cell Reports, vol. 35, no. 2, 108973. https://doi.org/10.1016/j.celrep.2021.108973

APA

Lund, V. K., Lycas, M. D., Schack, A., Andersen, R. C., Gether, U., & Kjaerulff, O. (2021). Rab2 drives axonal transport of dense core vesicles and lysosomal organelles. Cell Reports, 35(2), [108973]. https://doi.org/10.1016/j.celrep.2021.108973

Vancouver

Lund VK, Lycas MD, Schack A, Andersen RC, Gether U, Kjaerulff O. Rab2 drives axonal transport of dense core vesicles and lysosomal organelles. Cell Reports. 2021;35(2). 108973. https://doi.org/10.1016/j.celrep.2021.108973

Author

Lund, Viktor Karlovich ; Lycas, Matthew Domenic ; Schack, Anders ; Andersen, Rita Chan ; Gether, Ulrik ; Kjaerulff, Ole. / Rab2 drives axonal transport of dense core vesicles and lysosomal organelles. In: Cell Reports. 2021 ; Vol. 35, No. 2.

Bibtex

@article{7ceb48e3ac534ae68ec4716ea3227e0e,

title = "Rab2 drives axonal transport of dense core vesicles and lysosomal organelles",

abstract = "Fast axonal transport of neuropeptide-containing dense core vesicles (DCVs), endolysosomal organelles, and presynaptic components is critical for maintaining neuronal functionality. How the transport of DCVs is orchestrated remains an important unresolved question. The small GTPase Rab2 mediates DCV biogenesis and endosome-lysosome fusion. Here, we use Drosophila to demonstrate that Rab2 also plays a critical role in bidirectional axonal transport of DCVs, endosomes, and lysosomal organelles, most likely by controlling molecular motors. We further show that the lysosomal motility factor Arl8 is required as well for axonal transport of DCVs, but unlike Rab2, it is also critical for DCV exit from cell bodies into axons. We also provide evidence that the upstream regulators of Rab2 and Arl8, Ema and BORC, activate these GTPases during DCV transport. Our results uncover the mechanisms underlying axonal transport of DCVs and reveal surprising parallels between the regulation of DCV and lysosomal motility.",

keywords = "Arl8, axonal transport, dense core vesicles, Drosophila, endosomes, lysosomes, membrane trafficking, neuropeptides, Rab2, synaptogenesis",

author = "Lund, {Viktor Karlovich} and Lycas, {Matthew Domenic} and Anders Schack and Andersen, {Rita Chan} and Ulrik Gether and Ole Kjaerulff",

note = "Publisher Copyright: {\textcopyright} 2021 The Authors",

year = "2021",

doi = "10.1016/j.celrep.2021.108973",

language = "English",

volume = "35",

journal = "Cell Reports",

issn = "2211-1247",

publisher = "Cell Press",

number = "2",

}

RIS

TY - JOUR

T1 - Rab2 drives axonal transport of dense core vesicles and lysosomal organelles

AU - Lund, Viktor Karlovich

AU - Lycas, Matthew Domenic

AU - Schack, Anders

AU - Andersen, Rita Chan

AU - Gether, Ulrik

AU - Kjaerulff, Ole

PY - 2021

Y1 - 2021

N2 - Fast axonal transport of neuropeptide-containing dense core vesicles (DCVs), endolysosomal organelles, and presynaptic components is critical for maintaining neuronal functionality. How the transport of DCVs is orchestrated remains an important unresolved question. The small GTPase Rab2 mediates DCV biogenesis and endosome-lysosome fusion. Here, we use Drosophila to demonstrate that Rab2 also plays a critical role in bidirectional axonal transport of DCVs, endosomes, and lysosomal organelles, most likely by controlling molecular motors. We further show that the lysosomal motility factor Arl8 is required as well for axonal transport of DCVs, but unlike Rab2, it is also critical for DCV exit from cell bodies into axons. We also provide evidence that the upstream regulators of Rab2 and Arl8, Ema and BORC, activate these GTPases during DCV transport. Our results uncover the mechanisms underlying axonal transport of DCVs and reveal surprising parallels between the regulation of DCV and lysosomal motility.

AB - Fast axonal transport of neuropeptide-containing dense core vesicles (DCVs), endolysosomal organelles, and presynaptic components is critical for maintaining neuronal functionality. How the transport of DCVs is orchestrated remains an important unresolved question. The small GTPase Rab2 mediates DCV biogenesis and endosome-lysosome fusion. Here, we use Drosophila to demonstrate that Rab2 also plays a critical role in bidirectional axonal transport of DCVs, endosomes, and lysosomal organelles, most likely by controlling molecular motors. We further show that the lysosomal motility factor Arl8 is required as well for axonal transport of DCVs, but unlike Rab2, it is also critical for DCV exit from cell bodies into axons. We also provide evidence that the upstream regulators of Rab2 and Arl8, Ema and BORC, activate these GTPases during DCV transport. Our results uncover the mechanisms underlying axonal transport of DCVs and reveal surprising parallels between the regulation of DCV and lysosomal motility.

KW - Arl8

KW - axonal transport

KW - dense core vesicles

KW - Drosophila

KW - endosomes

KW - lysosomes

KW - membrane trafficking

KW - neuropeptides

KW - Rab2

KW - synaptogenesis

U2 - 10.1016/j.celrep.2021.108973

DO - 10.1016/j.celrep.2021.108973

M3 - Journal article

C2 - 33852866

AN - SCOPUS:85104084528

VL - 35

JO - Cell Reports

JF - Cell Reports

SN - 2211-1247

IS - 2

M1 - 108973

ER -

ID: 262938831

Department of Neuroscience