Prolonged life of human acute hippocampal slices from temporal lobe epilepsy surgery

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Prolonged life of human acute hippocampal slices from temporal lobe epilepsy surgery. / Wickham, J; Brödjegård, N G; Vighagen, R; Pinborg, L H; Bengzon, J; Woldbye, D P D; Kokaia, M; Andersson, M.

In: Scientific Reports, Vol. 8, 4158, 2018.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Wickham, J, Brödjegård, NG, Vighagen, R, Pinborg, LH, Bengzon, J, Woldbye, DPD, Kokaia, M & Andersson, M 2018, 'Prolonged life of human acute hippocampal slices from temporal lobe epilepsy surgery', Scientific Reports, vol. 8, 4158. https://doi.org/10.1038/s41598-018-22554-9

APA

Wickham, J., Brödjegård, N. G., Vighagen, R., Pinborg, L. H., Bengzon, J., Woldbye, D. P. D., Kokaia, M., & Andersson, M. (2018). Prolonged life of human acute hippocampal slices from temporal lobe epilepsy surgery. Scientific Reports, 8, [4158]. https://doi.org/10.1038/s41598-018-22554-9

Vancouver

Wickham J, Brödjegård NG, Vighagen R, Pinborg LH, Bengzon J, Woldbye DPD et al. Prolonged life of human acute hippocampal slices from temporal lobe epilepsy surgery. Scientific Reports. 2018;8. 4158. https://doi.org/10.1038/s41598-018-22554-9

Author

Wickham, J ; Brödjegård, N G ; Vighagen, R ; Pinborg, L H ; Bengzon, J ; Woldbye, D P D ; Kokaia, M ; Andersson, M. / Prolonged life of human acute hippocampal slices from temporal lobe epilepsy surgery. In: Scientific Reports. 2018 ; Vol. 8.

Bibtex

@article{b9129c777778424f8b6378d4ab286573,
title = "Prolonged life of human acute hippocampal slices from temporal lobe epilepsy surgery",
abstract = "Resected hippocampal tissue from patients with drug-resistant epilepsy presents a unique possibility to test novel treatment strategies directly in target tissue. The post-resection time for testing and analysis however is normally limited. Acute tissue slices allow for electrophysiological recordings typically up to 12 hours. To enable longer time to test novel treatment strategies such as, e.g., gene-therapy, we developed a method for keeping acute human brain slices viable over a longer period. Our protocol keeps neurons viable well up to 48 hours. Using a dual-flow chamber, which allows for microscopic visualisation of individual neurons with a submerged objective for whole-cell patch-clamp recordings, we report stable electrophysiological properties, such as action potential amplitude and threshold during this time. We also demonstrate that epileptiform activity, monitored by individual dentate granule whole-cell recordings, can be consistently induced in these slices, underlying the usefulness of this methodology for testing and/or validating novel treatment strategies for epilepsy.",
author = "J Wickham and Br{\"o}djeg{\aa}rd, {N G} and R Vighagen and Pinborg, {L H} and J Bengzon and Woldbye, {D P D} and M Kokaia and M Andersson",
year = "2018",
doi = "10.1038/s41598-018-22554-9",
language = "English",
volume = "8",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - Prolonged life of human acute hippocampal slices from temporal lobe epilepsy surgery

AU - Wickham, J

AU - Brödjegård, N G

AU - Vighagen, R

AU - Pinborg, L H

AU - Bengzon, J

AU - Woldbye, D P D

AU - Kokaia, M

AU - Andersson, M

PY - 2018

Y1 - 2018

N2 - Resected hippocampal tissue from patients with drug-resistant epilepsy presents a unique possibility to test novel treatment strategies directly in target tissue. The post-resection time for testing and analysis however is normally limited. Acute tissue slices allow for electrophysiological recordings typically up to 12 hours. To enable longer time to test novel treatment strategies such as, e.g., gene-therapy, we developed a method for keeping acute human brain slices viable over a longer period. Our protocol keeps neurons viable well up to 48 hours. Using a dual-flow chamber, which allows for microscopic visualisation of individual neurons with a submerged objective for whole-cell patch-clamp recordings, we report stable electrophysiological properties, such as action potential amplitude and threshold during this time. We also demonstrate that epileptiform activity, monitored by individual dentate granule whole-cell recordings, can be consistently induced in these slices, underlying the usefulness of this methodology for testing and/or validating novel treatment strategies for epilepsy.

AB - Resected hippocampal tissue from patients with drug-resistant epilepsy presents a unique possibility to test novel treatment strategies directly in target tissue. The post-resection time for testing and analysis however is normally limited. Acute tissue slices allow for electrophysiological recordings typically up to 12 hours. To enable longer time to test novel treatment strategies such as, e.g., gene-therapy, we developed a method for keeping acute human brain slices viable over a longer period. Our protocol keeps neurons viable well up to 48 hours. Using a dual-flow chamber, which allows for microscopic visualisation of individual neurons with a submerged objective for whole-cell patch-clamp recordings, we report stable electrophysiological properties, such as action potential amplitude and threshold during this time. We also demonstrate that epileptiform activity, monitored by individual dentate granule whole-cell recordings, can be consistently induced in these slices, underlying the usefulness of this methodology for testing and/or validating novel treatment strategies for epilepsy.

U2 - 10.1038/s41598-018-22554-9

DO - 10.1038/s41598-018-22554-9

M3 - Journal article

C2 - 29515159

VL - 8

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 4158

ER -

ID: 213166803