Presynaptic spinophilin tunes neurexin signalling to control active zone architecture and function

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Presynaptic spinophilin tunes neurexin signalling to control active zone architecture and function. / Muhammad, Karzan; Reddy-Alla, Suneel; Driller, Jan H; Schreiner, Dietmar; Rey, Ulises; Böhme, Mathias A; Hollmann, Christina; Ramesh, Niraja; Depner, Harald; Lützkendorf, Janine; Matkovic, Tanja; Götz, Torsten; Bergeron, Dominique D; Schmoranzer, Jan; Goettfert, Fabian; Holt, Mathew; Wahl, Markus C; Hell, Stefan W; Scheiffele, Peter; Walter, Alexander M; Loll, Bernhard; Sigrist, Stephan J.

In: Nature Communications, Vol. 6, 8362, 2015.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Muhammad, K, Reddy-Alla, S, Driller, JH, Schreiner, D, Rey, U, Böhme, MA, Hollmann, C, Ramesh, N, Depner, H, Lützkendorf, J, Matkovic, T, Götz, T, Bergeron, DD, Schmoranzer, J, Goettfert, F, Holt, M, Wahl, MC, Hell, SW, Scheiffele, P, Walter, AM, Loll, B & Sigrist, SJ 2015, 'Presynaptic spinophilin tunes neurexin signalling to control active zone architecture and function', Nature Communications, vol. 6, 8362. https://doi.org/10.1038/ncomms9362

APA

Muhammad, K., Reddy-Alla, S., Driller, J. H., Schreiner, D., Rey, U., Böhme, M. A., Hollmann, C., Ramesh, N., Depner, H., Lützkendorf, J., Matkovic, T., Götz, T., Bergeron, D. D., Schmoranzer, J., Goettfert, F., Holt, M., Wahl, M. C., Hell, S. W., Scheiffele, P., ... Sigrist, S. J. (2015). Presynaptic spinophilin tunes neurexin signalling to control active zone architecture and function. Nature Communications, 6, [8362]. https://doi.org/10.1038/ncomms9362

Vancouver

Muhammad K, Reddy-Alla S, Driller JH, Schreiner D, Rey U, Böhme MA et al. Presynaptic spinophilin tunes neurexin signalling to control active zone architecture and function. Nature Communications. 2015;6. 8362. https://doi.org/10.1038/ncomms9362

Author

Muhammad, Karzan ; Reddy-Alla, Suneel ; Driller, Jan H ; Schreiner, Dietmar ; Rey, Ulises ; Böhme, Mathias A ; Hollmann, Christina ; Ramesh, Niraja ; Depner, Harald ; Lützkendorf, Janine ; Matkovic, Tanja ; Götz, Torsten ; Bergeron, Dominique D ; Schmoranzer, Jan ; Goettfert, Fabian ; Holt, Mathew ; Wahl, Markus C ; Hell, Stefan W ; Scheiffele, Peter ; Walter, Alexander M ; Loll, Bernhard ; Sigrist, Stephan J. / Presynaptic spinophilin tunes neurexin signalling to control active zone architecture and function. In: Nature Communications. 2015 ; Vol. 6.

Bibtex

@article{9bd8f52481ad4c32909a013b1d9780ff,
title = "Presynaptic spinophilin tunes neurexin signalling to control active zone architecture and function",
abstract = "Assembly and maturation of synapses at the Drosophila neuromuscular junction (NMJ) depend on trans-synaptic neurexin/neuroligin signalling, which is promoted by the scaffolding protein Syd-1 binding to neurexin. Here we report that the scaffold protein spinophilin binds to the C-terminal portion of neurexin and is needed to limit neurexin/neuroligin signalling by acting antagonistic to Syd-1. Loss of presynaptic spinophilin results in the formation of excess, but atypically small active zones. Neuroligin-1/neurexin-1/Syd-1 levels are increased at spinophilin mutant NMJs, and removal of single copies of the neurexin-1, Syd-1 or neuroligin-1 genes suppresses the spinophilin-active zone phenotype. Evoked transmission is strongly reduced at spinophilin terminals, owing to a severely reduced release probability at individual active zones. We conclude that presynaptic spinophilin fine-tunes neurexin/neuroligin signalling to control active zone number and functionality, thereby optimizing them for action potential-induced exocytosis.",
keywords = "Animals, Cell Adhesion Molecules, Neuronal/metabolism, Drosophila, Drosophila Proteins/metabolism, Female, GTPase-Activating Proteins/metabolism, Male, Microfilament Proteins/metabolism, Nerve Tissue Proteins/metabolism, PDZ Domains, Synapses/metabolism",
author = "Karzan Muhammad and Suneel Reddy-Alla and Driller, {Jan H} and Dietmar Schreiner and Ulises Rey and B{\"o}hme, {Mathias A} and Christina Hollmann and Niraja Ramesh and Harald Depner and Janine L{\"u}tzkendorf and Tanja Matkovic and Torsten G{\"o}tz and Bergeron, {Dominique D} and Jan Schmoranzer and Fabian Goettfert and Mathew Holt and Wahl, {Markus C} and Hell, {Stefan W} and Peter Scheiffele and Walter, {Alexander M} and Bernhard Loll and Sigrist, {Stephan J}",
year = "2015",
doi = "10.1038/ncomms9362",
language = "English",
volume = "6",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - Presynaptic spinophilin tunes neurexin signalling to control active zone architecture and function

AU - Muhammad, Karzan

AU - Reddy-Alla, Suneel

AU - Driller, Jan H

AU - Schreiner, Dietmar

AU - Rey, Ulises

AU - Böhme, Mathias A

AU - Hollmann, Christina

AU - Ramesh, Niraja

AU - Depner, Harald

AU - Lützkendorf, Janine

AU - Matkovic, Tanja

AU - Götz, Torsten

AU - Bergeron, Dominique D

AU - Schmoranzer, Jan

AU - Goettfert, Fabian

AU - Holt, Mathew

AU - Wahl, Markus C

AU - Hell, Stefan W

AU - Scheiffele, Peter

AU - Walter, Alexander M

AU - Loll, Bernhard

AU - Sigrist, Stephan J

PY - 2015

Y1 - 2015

N2 - Assembly and maturation of synapses at the Drosophila neuromuscular junction (NMJ) depend on trans-synaptic neurexin/neuroligin signalling, which is promoted by the scaffolding protein Syd-1 binding to neurexin. Here we report that the scaffold protein spinophilin binds to the C-terminal portion of neurexin and is needed to limit neurexin/neuroligin signalling by acting antagonistic to Syd-1. Loss of presynaptic spinophilin results in the formation of excess, but atypically small active zones. Neuroligin-1/neurexin-1/Syd-1 levels are increased at spinophilin mutant NMJs, and removal of single copies of the neurexin-1, Syd-1 or neuroligin-1 genes suppresses the spinophilin-active zone phenotype. Evoked transmission is strongly reduced at spinophilin terminals, owing to a severely reduced release probability at individual active zones. We conclude that presynaptic spinophilin fine-tunes neurexin/neuroligin signalling to control active zone number and functionality, thereby optimizing them for action potential-induced exocytosis.

AB - Assembly and maturation of synapses at the Drosophila neuromuscular junction (NMJ) depend on trans-synaptic neurexin/neuroligin signalling, which is promoted by the scaffolding protein Syd-1 binding to neurexin. Here we report that the scaffold protein spinophilin binds to the C-terminal portion of neurexin and is needed to limit neurexin/neuroligin signalling by acting antagonistic to Syd-1. Loss of presynaptic spinophilin results in the formation of excess, but atypically small active zones. Neuroligin-1/neurexin-1/Syd-1 levels are increased at spinophilin mutant NMJs, and removal of single copies of the neurexin-1, Syd-1 or neuroligin-1 genes suppresses the spinophilin-active zone phenotype. Evoked transmission is strongly reduced at spinophilin terminals, owing to a severely reduced release probability at individual active zones. We conclude that presynaptic spinophilin fine-tunes neurexin/neuroligin signalling to control active zone number and functionality, thereby optimizing them for action potential-induced exocytosis.

KW - Animals

KW - Cell Adhesion Molecules, Neuronal/metabolism

KW - Drosophila

KW - Drosophila Proteins/metabolism

KW - Female

KW - GTPase-Activating Proteins/metabolism

KW - Male

KW - Microfilament Proteins/metabolism

KW - Nerve Tissue Proteins/metabolism

KW - PDZ Domains

KW - Synapses/metabolism

U2 - 10.1038/ncomms9362

DO - 10.1038/ncomms9362

M3 - Journal article

C2 - 26471740

VL - 6

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

M1 - 8362

ER -

ID: 334035837