Presence of the Endocannabinoid System in the Inferior Pulvinar of the Vervet Monkey
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Presence of the Endocannabinoid System in the Inferior Pulvinar of the Vervet Monkey. / Micaelo-Fernandes, Catarina; Bouskila, Joseph; Bouchard, Jean-Francois; Ptito, Maurice.
In: Brain Sciences, Vol. 11, No. 6, 770, 2021.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - Presence of the Endocannabinoid System in the Inferior Pulvinar of the Vervet Monkey
AU - Micaelo-Fernandes, Catarina
AU - Bouskila, Joseph
AU - Bouchard, Jean-Francois
AU - Ptito, Maurice
PY - 2021
Y1 - 2021
N2 - The expression of the endocannabinoid (eCB) system, including cannabinoid receptor type 1 (CB1R) and the cannabinoid synthesizing (NAPE-PLD) and degrading (FAAH) enzymes, has been well-characterized in the retina of rodents and monkeys. More recently, the presence of CB1R was localized throughout the dorsal lateral geniculate nucleus of the thalamus of vervet monkeys. Given that the retina projects also to the pulvinar either via a direct projection or via the superior colliculus, it was reasonable to assume that this system would be present therein. The visual pulvinar, namely the inferior pulvinar (PI) region, was delineated with calbindin immunohistochemical staining. Using Western blots and immunofluorescence, we demonstrated that CB1R, NAPE-PLD and FAAH are expressed in the PI of the vervet monkey. Throughout the PI, CB1R was mainly colocalized with VGLUT2-positive axon terminals in the vicinity of calbindin and parvalbumin-positive neurons. NAPE-PLD and FAAH rather colocalized with calbindin over the somatodendritic compartment of PI neurons. Our results suggest that visual information coming from the retina and entering the PI is modulated by the eCB system on its way to the dorsal visual stream. These results provide insights for understanding the role of eCBs in the modulation of visual thalamic inputs and, hence, visual perception.
AB - The expression of the endocannabinoid (eCB) system, including cannabinoid receptor type 1 (CB1R) and the cannabinoid synthesizing (NAPE-PLD) and degrading (FAAH) enzymes, has been well-characterized in the retina of rodents and monkeys. More recently, the presence of CB1R was localized throughout the dorsal lateral geniculate nucleus of the thalamus of vervet monkeys. Given that the retina projects also to the pulvinar either via a direct projection or via the superior colliculus, it was reasonable to assume that this system would be present therein. The visual pulvinar, namely the inferior pulvinar (PI) region, was delineated with calbindin immunohistochemical staining. Using Western blots and immunofluorescence, we demonstrated that CB1R, NAPE-PLD and FAAH are expressed in the PI of the vervet monkey. Throughout the PI, CB1R was mainly colocalized with VGLUT2-positive axon terminals in the vicinity of calbindin and parvalbumin-positive neurons. NAPE-PLD and FAAH rather colocalized with calbindin over the somatodendritic compartment of PI neurons. Our results suggest that visual information coming from the retina and entering the PI is modulated by the eCB system on its way to the dorsal visual stream. These results provide insights for understanding the role of eCBs in the modulation of visual thalamic inputs and, hence, visual perception.
KW - vervet monkeys
KW - pulvinar
KW - endocannabinoids
KW - CB1R
KW - FAAH
KW - NAPE-PLD
KW - vision
KW - ACID AMIDE HYDROLASE
KW - MIDDLE TEMPORAL AREA
KW - SUPERIOR COLLICULUS
KW - EARLY MATURATION
KW - VISUAL-CORTEX
KW - NEW-WORLD
KW - LOCALIZATION
KW - PROJECTIONS
KW - SUBDIVISIONS
KW - EXPRESSION
U2 - 10.3390/brainsci11060770
DO - 10.3390/brainsci11060770
M3 - Journal article
C2 - 34200554
VL - 11
JO - Brain Sciences
JF - Brain Sciences
SN - 2076-3425
IS - 6
M1 - 770
ER -
ID: 273531213