Phrenic-specific transcriptional programs shape respiratory motor output

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Phrenic-specific transcriptional programs shape respiratory motor output. / Vagnozzi, Alicia N; Garg, Kiran; Dewitz, Carola; Moore, Matthew T; Cregg, Jared M; Jeannotte, Lucie; Zampieri, Niccolò; Landmesser, Lynn T; Philippidou, Polyxeni.

In: eLife, Vol. 9, e52859, 16.01.2020.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Vagnozzi, AN, Garg, K, Dewitz, C, Moore, MT, Cregg, JM, Jeannotte, L, Zampieri, N, Landmesser, LT & Philippidou, P 2020, 'Phrenic-specific transcriptional programs shape respiratory motor output', eLife, vol. 9, e52859. https://doi.org/10.7554/eLife.52859

APA

Vagnozzi, A. N., Garg, K., Dewitz, C., Moore, M. T., Cregg, J. M., Jeannotte, L., Zampieri, N., Landmesser, L. T., & Philippidou, P. (2020). Phrenic-specific transcriptional programs shape respiratory motor output. eLife, 9, [e52859]. https://doi.org/10.7554/eLife.52859

Vancouver

Vagnozzi AN, Garg K, Dewitz C, Moore MT, Cregg JM, Jeannotte L et al. Phrenic-specific transcriptional programs shape respiratory motor output. eLife. 2020 Jan 16;9. e52859. https://doi.org/10.7554/eLife.52859

Author

Vagnozzi, Alicia N ; Garg, Kiran ; Dewitz, Carola ; Moore, Matthew T ; Cregg, Jared M ; Jeannotte, Lucie ; Zampieri, Niccolò ; Landmesser, Lynn T ; Philippidou, Polyxeni. / Phrenic-specific transcriptional programs shape respiratory motor output. In: eLife. 2020 ; Vol. 9.

Bibtex

@article{9cdf1b001542492e8c3a6aec37affc2c,

title = "Phrenic-specific transcriptional programs shape respiratory motor output",

abstract = "The precise pattern of motor neuron (MN) activation is essential for the execution of motor actions; however, the molecular mechanisms that give rise to specific patterns of MN activity are largely unknown. Phrenic MNs integrate multiple inputs to mediate inspiratory activity during breathing and are constrained to fire in a pattern that drives efficient diaphragm contraction. We show that Hox5 transcription factors shape phrenic MN output by connecting phrenic MNs to inhibitory premotor neurons. Hox5 genes establish phrenic MN organization and dendritic topography through the regulation of phrenic-specific cell adhesion programs. In the absence of Hox5 genes, phrenic MN firing becomes asynchronous and erratic due to loss of phrenic MN inhibition. Strikingly, mice lacking Hox5 genes in MNs exhibit abnormal respiratory behavior throughout their lifetime. Our findings support a model where MN-intrinsic transcriptional programs shape the pattern of motor output by orchestrating distinct aspects of MN connectivity.",

author = "Vagnozzi, {Alicia N} and Kiran Garg and Carola Dewitz and Moore, {Matthew T} and Cregg, {Jared M} and Lucie Jeannotte and Niccol{\`o} Zampieri and Landmesser, {Lynn T} and Polyxeni Philippidou",

note = "{\textcopyright} 2020, Vagnozzi et al.",

year = "2020",

month = jan,

day = "16",

doi = "10.7554/eLife.52859",

language = "English",

volume = "9",

journal = "eLife",

issn = "2050-084X",

publisher = "eLife Sciences Publications Ltd.",

}

RIS

TY - JOUR

T1 - Phrenic-specific transcriptional programs shape respiratory motor output

AU - Vagnozzi, Alicia N

AU - Garg, Kiran

AU - Dewitz, Carola

AU - Moore, Matthew T

AU - Cregg, Jared M

AU - Jeannotte, Lucie

AU - Zampieri, Niccolò

AU - Landmesser, Lynn T

AU - Philippidou, Polyxeni

PY - 2020/1/16

Y1 - 2020/1/16

N2 - The precise pattern of motor neuron (MN) activation is essential for the execution of motor actions; however, the molecular mechanisms that give rise to specific patterns of MN activity are largely unknown. Phrenic MNs integrate multiple inputs to mediate inspiratory activity during breathing and are constrained to fire in a pattern that drives efficient diaphragm contraction. We show that Hox5 transcription factors shape phrenic MN output by connecting phrenic MNs to inhibitory premotor neurons. Hox5 genes establish phrenic MN organization and dendritic topography through the regulation of phrenic-specific cell adhesion programs. In the absence of Hox5 genes, phrenic MN firing becomes asynchronous and erratic due to loss of phrenic MN inhibition. Strikingly, mice lacking Hox5 genes in MNs exhibit abnormal respiratory behavior throughout their lifetime. Our findings support a model where MN-intrinsic transcriptional programs shape the pattern of motor output by orchestrating distinct aspects of MN connectivity.

AB - The precise pattern of motor neuron (MN) activation is essential for the execution of motor actions; however, the molecular mechanisms that give rise to specific patterns of MN activity are largely unknown. Phrenic MNs integrate multiple inputs to mediate inspiratory activity during breathing and are constrained to fire in a pattern that drives efficient diaphragm contraction. We show that Hox5 transcription factors shape phrenic MN output by connecting phrenic MNs to inhibitory premotor neurons. Hox5 genes establish phrenic MN organization and dendritic topography through the regulation of phrenic-specific cell adhesion programs. In the absence of Hox5 genes, phrenic MN firing becomes asynchronous and erratic due to loss of phrenic MN inhibition. Strikingly, mice lacking Hox5 genes in MNs exhibit abnormal respiratory behavior throughout their lifetime. Our findings support a model where MN-intrinsic transcriptional programs shape the pattern of motor output by orchestrating distinct aspects of MN connectivity.

U2 - 10.7554/eLife.52859

DO - 10.7554/eLife.52859

M3 - Journal article

C2 - 31944180

VL - 9

JO - eLife

JF - eLife

SN - 2050-084X

M1 - e52859

ER -

ID: 248113721

Department of Neuroscience