Npas4, a novel helix-loop-helix PAS domain protein, is regulated in response to cerebral ischemia

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Npas4, a novel helix-loop-helix PAS domain protein, is regulated in response to cerebral ischemia. / Shamloo, Mehrdad; Soriano, Liza; von Schack, David; Rickhag, Karl Mattias; Chin, Daniel J; Gonzalez-Zulueta, Mirella; Gido, Gunilla; Urfer, Roman; Wieloch, Tadeusz; Nikolich, Karoly.

In: European Journal of Neuroscience, Vol. 24, No. 10, 11.2006, p. 2705-20.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Shamloo, M, Soriano, L, von Schack, D, Rickhag, KM, Chin, DJ, Gonzalez-Zulueta, M, Gido, G, Urfer, R, Wieloch, T & Nikolich, K 2006, 'Npas4, a novel helix-loop-helix PAS domain protein, is regulated in response to cerebral ischemia', European Journal of Neuroscience, vol. 24, no. 10, pp. 2705-20. https://doi.org/10.1111/j.1460-9568.2006.05172.x

APA

Shamloo, M., Soriano, L., von Schack, D., Rickhag, K. M., Chin, D. J., Gonzalez-Zulueta, M., Gido, G., Urfer, R., Wieloch, T., & Nikolich, K. (2006). Npas4, a novel helix-loop-helix PAS domain protein, is regulated in response to cerebral ischemia. European Journal of Neuroscience, 24(10), 2705-20. https://doi.org/10.1111/j.1460-9568.2006.05172.x

Vancouver

Shamloo M, Soriano L, von Schack D, Rickhag KM, Chin DJ, Gonzalez-Zulueta M et al. Npas4, a novel helix-loop-helix PAS domain protein, is regulated in response to cerebral ischemia. European Journal of Neuroscience. 2006 Nov;24(10):2705-20. https://doi.org/10.1111/j.1460-9568.2006.05172.x

Author

Shamloo, Mehrdad ; Soriano, Liza ; von Schack, David ; Rickhag, Karl Mattias ; Chin, Daniel J ; Gonzalez-Zulueta, Mirella ; Gido, Gunilla ; Urfer, Roman ; Wieloch, Tadeusz ; Nikolich, Karoly. / Npas4, a novel helix-loop-helix PAS domain protein, is regulated in response to cerebral ischemia. In: European Journal of Neuroscience. 2006 ; Vol. 24, No. 10. pp. 2705-20.

Bibtex

@article{fa4c39a719a24263a0174fecdfacabf4,
title = "Npas4, a novel helix-loop-helix PAS domain protein, is regulated in response to cerebral ischemia",
abstract = "Basic helix-loop-helix PAS domain proteins form a growing family of transcription factors. These proteins are involved in the process of adaptation to cellular stresses and environmental factors such as a change in oxygen concentration. We describe the identification and characterization of a recently cloned PAS domain protein termed Npas4 in ischemic rat brain. Using gene expression profiling following middle cerebral artery occlusion, we showed that the Npas4 mRNA is differentially expressed in ischemic tissue. The full-length gene was cloned from rat brain and its spatial and temporal expression characterized with in situ hybridization and Northern blotting. The Npas4 mRNA is specifically expressed in the brain and is highly up-regulated in ischemic tissues following both focal and global cerebral ischemic insults. Immunohistochemistry revealed a strong expression in the limbic system and thalamus, as well as in layers 3 and 5 in the cortex of the unchallenged brain. When overexpressed in HEK 293 cells, Npas4 appears as a protein of approximately 100 kDa. In brain samples, however, in addition to the 100 kDa band a specific 200 kDa immunoreactive band was also detected. Ischemic challenge lead to a decrease in the 200 kDa form and a simultaneous increase in the 100 kDa immunoreactivity. This could indicate a novel regulatory mechanism for activation and/or deactivation of this protein in response to ischemic brain injury.",
keywords = "Animals, Basic Helix-Loop-Helix Transcription Factors, Blotting, Northern, Blotting, Western, Brain, Cells, Cultured, Embryo, Mammalian, Gene Expression Regulation, Helix-Loop-Helix Motifs, Immunohistochemistry, In Situ Hybridization, Infarction, Middle Cerebral Artery, Male, Nerve Tissue Proteins, Neurons, RNA, Messenger, Rats, Rats, Wistar, Reverse Transcriptase Polymerase Chain Reaction, Subcellular Fractions, Synaptophysin, Time Factors",
author = "Mehrdad Shamloo and Liza Soriano and {von Schack}, David and Rickhag, {Karl Mattias} and Chin, {Daniel J} and Mirella Gonzalez-Zulueta and Gunilla Gido and Roman Urfer and Tadeusz Wieloch and Karoly Nikolich",
year = "2006",
month = nov,
doi = "10.1111/j.1460-9568.2006.05172.x",
language = "English",
volume = "24",
pages = "2705--20",
journal = "European Journal of Neuroscience",
issn = "0953-816X",
publisher = "Wiley-Blackwell",
number = "10",

}

RIS

TY - JOUR

T1 - Npas4, a novel helix-loop-helix PAS domain protein, is regulated in response to cerebral ischemia

AU - Shamloo, Mehrdad

AU - Soriano, Liza

AU - von Schack, David

AU - Rickhag, Karl Mattias

AU - Chin, Daniel J

AU - Gonzalez-Zulueta, Mirella

AU - Gido, Gunilla

AU - Urfer, Roman

AU - Wieloch, Tadeusz

AU - Nikolich, Karoly

PY - 2006/11

Y1 - 2006/11

N2 - Basic helix-loop-helix PAS domain proteins form a growing family of transcription factors. These proteins are involved in the process of adaptation to cellular stresses and environmental factors such as a change in oxygen concentration. We describe the identification and characterization of a recently cloned PAS domain protein termed Npas4 in ischemic rat brain. Using gene expression profiling following middle cerebral artery occlusion, we showed that the Npas4 mRNA is differentially expressed in ischemic tissue. The full-length gene was cloned from rat brain and its spatial and temporal expression characterized with in situ hybridization and Northern blotting. The Npas4 mRNA is specifically expressed in the brain and is highly up-regulated in ischemic tissues following both focal and global cerebral ischemic insults. Immunohistochemistry revealed a strong expression in the limbic system and thalamus, as well as in layers 3 and 5 in the cortex of the unchallenged brain. When overexpressed in HEK 293 cells, Npas4 appears as a protein of approximately 100 kDa. In brain samples, however, in addition to the 100 kDa band a specific 200 kDa immunoreactive band was also detected. Ischemic challenge lead to a decrease in the 200 kDa form and a simultaneous increase in the 100 kDa immunoreactivity. This could indicate a novel regulatory mechanism for activation and/or deactivation of this protein in response to ischemic brain injury.

AB - Basic helix-loop-helix PAS domain proteins form a growing family of transcription factors. These proteins are involved in the process of adaptation to cellular stresses and environmental factors such as a change in oxygen concentration. We describe the identification and characterization of a recently cloned PAS domain protein termed Npas4 in ischemic rat brain. Using gene expression profiling following middle cerebral artery occlusion, we showed that the Npas4 mRNA is differentially expressed in ischemic tissue. The full-length gene was cloned from rat brain and its spatial and temporal expression characterized with in situ hybridization and Northern blotting. The Npas4 mRNA is specifically expressed in the brain and is highly up-regulated in ischemic tissues following both focal and global cerebral ischemic insults. Immunohistochemistry revealed a strong expression in the limbic system and thalamus, as well as in layers 3 and 5 in the cortex of the unchallenged brain. When overexpressed in HEK 293 cells, Npas4 appears as a protein of approximately 100 kDa. In brain samples, however, in addition to the 100 kDa band a specific 200 kDa immunoreactive band was also detected. Ischemic challenge lead to a decrease in the 200 kDa form and a simultaneous increase in the 100 kDa immunoreactivity. This could indicate a novel regulatory mechanism for activation and/or deactivation of this protein in response to ischemic brain injury.

KW - Animals

KW - Basic Helix-Loop-Helix Transcription Factors

KW - Blotting, Northern

KW - Blotting, Western

KW - Brain

KW - Cells, Cultured

KW - Embryo, Mammalian

KW - Gene Expression Regulation

KW - Helix-Loop-Helix Motifs

KW - Immunohistochemistry

KW - In Situ Hybridization

KW - Infarction, Middle Cerebral Artery

KW - Male

KW - Nerve Tissue Proteins

KW - Neurons

KW - RNA, Messenger

KW - Rats

KW - Rats, Wistar

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Subcellular Fractions

KW - Synaptophysin

KW - Time Factors

U2 - 10.1111/j.1460-9568.2006.05172.x

DO - 10.1111/j.1460-9568.2006.05172.x

M3 - Journal article

C2 - 17156197

VL - 24

SP - 2705

EP - 2720

JO - European Journal of Neuroscience

JF - European Journal of Neuroscience

SN - 0953-816X

IS - 10

ER -

ID: 132931360