NK-3 receptor activation depolarizes and induces an after-depolarization in pyramidal neurons in gerbil cingulate cortex
Research output: Contribution to journal › Journal article › Research › peer-review
The involvement of tachykinins in cortical function is poorly understood. To study the actions of neurokinin-3 (NK3) receptor activation in frontal cortex, whole cell patch clamp recordings were performed from pyramidal neurons in slices of cingulate cortex from juvenile gerbils. Senktide (500nM), a selective NK3 receptor agonist, induced a transient increase in spontaneous EPSPs in layer V pyramidal neurons, accompanied by a small depolarization ( approximately 4 mV). EPSPs during senktide had a larger amplitude and faster 10-90% rise time than during control. Senktide induced a transient depolarization in layer II/III pyramidal neurons, which often reached threshold for spikes. The depolarization ( approximately 6 mV) persisted in TTX, and was accompanied by an increase in input resistance. Senktide also transiently induced a slow after-depolarization, which appeared following a depolarizing pulse. The slow after-depolarization persisted in TTX. These data suggest that activation of NK3 receptors on layer II/III pyramidal neurons induce post-synaptic depolarization and an after-depolarization, which could be mediated by blockade of a leak potassium conductance and a non-selective cation conductance, respectively.
Original language | English |
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Journal | Brain Research Bulletin |
Volume | 63 |
Issue number | 2 |
Pages (from-to) | 85-90 |
Number of pages | 5 |
ISSN | 0361-9230 |
DOIs | |
Publication status | Published - 2004 |
Bibliographical note
Keywords: Animals; Cerebral Cortex; Excitatory Postsynaptic Potentials; Female; Gerbillinae; Gyrus Cinguli; Male; Peptide Fragments; Pyramidal Cells; Receptors, Neurokinin-3; Substance P
ID: 9255713